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| 1 | Genetic diversity and population structure analysis of Capsicum germplasm accessions显示文摘Genetic diversity plays an essential role in plant breeding and utilization.Pepper is an important vegetable and spice crop worldwide.The genetic diversity of 1 904 accessions of pepper conserved at the National Mid-term Genebank for Vegetables,Beijing,China was analyzed based on 29 simple sequence repeat(SSR)markers,which were evenly distributed over 12 pepper chromosomes.The pepper accessions were divided into two groups in a genetic structure analysis,and the two groups showed obvious differences in fruit type and geographical distribution.We finally selected 248 accessions capturing 75.6%of the SSR alleles as the core collection for further research.Insights into the genetic structure of pepper provide the basis for population-level gene mining and genetic improvement. | GU Xiao-zhen CAO Ya-cong ZHANG Zheng-hai ZHANG Bao-xi ZHAO Hong ZHANG Xiao-min WANG Hai-ping LI Xi-xiang WANG Li-hao | 2019 | Journal of Integrative Agriculture2019，18，6： | 10 |
| 2 | 早春辣椒连作免耕栽培技术显示文摘辣椒(Capsicum annuum L.)俗称"辣子",是全世界消费量最大的蔬菜之一,我国是居全球之首的辣椒生产和消费大国,辣椒在近几十年其贸易量超过咖啡与茶叶。据不完全统计,全国辣椒播种面积已超过140多万hm2,产量2 700万t,实现产值270亿元,占据我国蔬菜作物种植面积的1/10多。然而,早春辣椒种植主要在大棚或温室等保护地进行,由于设施数量有限,加之土地、气候等条件的限制,以及对高产的追求,连续在同一地块种植辣椒,得不到倒茬轮作的机会,此外工业污染、尾气排放及化肥农药的过量使用,导致辣椒栽培的连作障碍。 | 孙国胜 马志虎 孙春青 戴忠良 毛忠良 潘跃平 | 2015 | 中国瓜菜2015，28，1： | 6 |
| 3 | 云南文山小米辣栽培情况调查及其主售品种评价显示文摘文山地区小米辣种植面积达4.7万hm^2(70万亩),为云南省最大的小米辣原料生产基地。本文对文山小米辣的生物学特性、栽培模式、市场主售品种状况进行了调研,并针对生产上存在的问题提出发展对策。辣椒是我国主要的蔬菜兼调味品,因其适应性广、营养丰富、产业链长,栽培面积迅速扩大。据农业部大宗蔬菜体系统计,近年来我国辣椒年播种面积在150万~200万hm^2,占全国蔬菜总播种面积的8%~10%,居蔬菜首位(王立浩等,2016)。 | 屈用函 袁恩平 王绍祥 赵水灵 杨羚钰 李云 张雪廷 | 2016 | 中国蔬菜2016，，11： | 6 |
| 4 | Reference quality assembly of the 3.5-Gb genome of Capsicum annuum from a single linked-read library显示文摘Linked-Read sequencing technology has recently been employed successfully for de novo assembly of human genomes,however,the utility of this technology for complex plant genomes is unproven.We evaluated the technology for this purpose by sequencing the 3.5-gigabase(Gb)diploid pepper(Capsicum annuum)genome with a single Linked-Read library.Plant genomes,including pepper,are characterized by long,highly similar repetitive sequences.Accordingly,significant effort is used to ensure that the sequenced plant is highly homozygous and the resulting assembly is a haploid consensus.With a phased assembly approach,we targeted a heterozygous F1 derived from a wide cross to assess the ability to derive both haplotypes and characterize a pungency gene with a large insertion/deletion.The Supernova software generated a highly ordered,more contiguous sequence assembly than all currently available C.annuum reference genomes.Over 83%of the final assembly was anchored and oriented using four publicly available de novo linkage maps.A comparison of the annotation of conserved eukaryotic genes indicated the completeness of assembly.The validity of the phased assembly is further demonstrated with the complete recovery of both 2.5-Kb insertion/deletion haplotypes of the PUN1 locus in the F1 sample that represents pungent and nonpungent peppers,as well as nearly full recovery of the BUSCO2 gene set within each of the two haplotypes.The most contiguous pepper genome assembly to date has been generated which demonstrates that Linked-Read library technology provides a tool to de novo assemble complex highly repetitive heterozygous plant genomes.This technology can provide an opportunity to cost-effectively develop high-quality genome assemblies for other complex plants and compare structural and gene differences through accurate haplotype reconstruction. | Amanda M.Hulse-Kemp Shamoni Maheshwari Kevin Stoffel Theresa A.Hill David Jaffe Stephen R.Williams Neil Weisenfeld Srividya Ramakrishnan Vijay Kumar Preyas Shah Michael C.Schatz Deanna M.Church Allen Van Deynze | 2018 | Horticulture Research2018，5，1： | 4 |
| 5 | 莴苣褪绿心腐病综合防控技术显示文摘2005年国内首次报道的莴苣褪绿心腐病是由黑额二叉叶蝉等媒介昆虫传播的一种植原体病害,通过适当推迟播种期、远离辣椒地育苗、防虫网覆盖育苗,及时拔除病株、悬挂黄板诱杀和适时喷药防治虫媒等系列治虫防病的综合防控措施可以有效地控制该病害的发生为害。 | 林积秀 严叔平 纪翠红 黄培枝 刘建密 陈彩霞 陈文乐 | 2016 | 长江蔬菜2016，0，11： | 3 |
| 6 | 一个与辣椒细胞核雄性不育性状连锁的高分辨率熔解曲线(HRM)标记的开发显示文摘雄性不育性状被用在植物育种中,以降低种子生产成本。雄性不育分为细胞核雄性不育(GMS)和细胞质雄性不育(CMS)2种类型,在辣椒商业育种中都有应用。因为缺少恢复源,细胞质雄性不育系统在诸如甜椒等一些辣椒品种上并不适用。因此,细胞核雄性不育对于甜椒的种子生产而言就非常重要。本研究构建了一个F2分离群体,利用集群分离分析法(BSA)和扩增片段长度多态性(AFLP)技术获得了一个与甜椒细胞核雄性不育连锁的标记。我们分析了1024对AFLP引物,发现Eco RI ACG/MseIGTT引物扩出了多态性。对多态性AFLP标记所扩增的395bp片段重新设计引物,进行高分辨率熔解曲线分析(HRM)。在92个F2单株中,有87个单株的性状和HRM标记表型相一致。这个HRM标记被定位在AC99连锁图的第5号染色体上。 | Heung-Ryul Lee Hye Jung An Dong Cheol Yang Soon Ho Choi Hyoun-Joung Kim Han Gheol Rhee Chee Hark Harn 孔秋生 | 2012 | 辣椒杂志2012，，4： | 3 |
| 7 | 海南三亚南繁基地冬季辣椒人工去雄杂交制种技术显示文摘辣椒(Capsicum annuum L.)为茄科辣椒属一年生草本植物,按植株形态和市场,一般分为甜椒(无辣味的辣椒)和辣椒(有辣味的辣椒)两大类型。辣椒属常异花授粉作物,通过利用两个纯合的品种或品系通过杂交产生的杂种一代在熟性、丰产性、抗病和抗逆性方面具有十分明显的杂种优势,表现为产量高,品质好,抗逆性强等。辣椒杂交制种保证亲本种子质量是关键。 | 陈斌 张晓芬 耿三省 耿传义 | 2014 | 辣椒杂志2014，，2： | 3 |
| 8 | 镉胁迫下连作辣椒的生长与光合特性显示文摘辣椒(Capsicum annuum L.)是一种重要的茄果类蔬菜,营养价值高,并且从辣椒中提取出来的辣椒碱、辣椒红色素及辣椒籽油在食品、医疗、军事、化妆品等行业中应用广泛,使辣椒成为一种世界性的仅次于豆类、番茄的第三大蔬菜作物。据不完全统计,我国辣椒的种植面积约为133万公顷,占世界辣椒面积的35%,仅次于我国白菜类蔬菜的栽培面积。然而,由于土地、气候等条件的限制以及对高产高效的追求,辣椒连作较为普遍。 | 万晋麟 侯永侠 杨晶 宋雪英 李玉双 杨继松 | 2016 | 新农业2016，，11： | 3 |
| 9 | 生物菌肥对辣椒和土壤肥力影响初探显示文摘为了探索生物菌肥的效应和合理施用,在保护地施用生物菌肥后栽植辣椒,分析测定施用纯化肥、未发酵型生物菌肥和发酵型生物菌肥下的辣椒产量、经济效益、品质和土壤养分含量。结果表明,在增产增收效果方面,发酵型生物菌肥明显优于未发酵型生物菌肥。生物菌肥还对抑制辣椒亚硝酸盐积累、提升辣椒Vc含量、调节土壤pH值、增加土壤放线菌数量、提高土壤速效磷含量有一定作用。发酵型生物菌肥能提升土壤速效钾、碱解氮含量。 | 臧延东 梁运江 许广波 赵洪颜 | 2014 | 中国园艺文摘2014，30，7： | 2 |
| 10 | 青椒(Capsicum frutescens var.grossum)“雄AB_(18)”的选育和利用显示文摘青椒的杂种优势非常显著,但因花器较小,人工去雄制杂交种时非常不便,为了解决生产中存在的这一难题,本研究以'克山大辣椒雄性不育两用系'为不育源,以本地早熟甜椒优良亲本材料'7906'为转育父本,采用'二环系'的转育方法为尽快地育成适于本地区生产利用的新青椒雄性不育系,自1985年以来做了如下的工作。1 '雄 AB18'的选育1985年在山西省农科院蔬菜所试验地,种植'克山大辣椒雄性不育两用系',其不育率为50%左右,选择其开花早,雄性不育性状典型的植株为母本。同时种植本地亲本材料'7906'选择其开花早,果实大,经济性状好的植株为父本成对杂交。 | 蒋伟明 侯仕熙 周林 董晓飞 郭伟民 | 1993 | 中国农业大学学报1993，，S4： | 2 |
| 11 | Effects of cucumber mosaic virus-infected chilli plants on non-vector Bemisia tabaci (Hemiptera Aleyrodidae)显示文摘Plant virus infections are known to alter host plant attractiveness and suitability for insect herbivores.This study was conducted to determine how cucumber mosaic virus (CMV)-infected chilli plants affect the fitness and settling preferences ofnonvector whitefly,Bemisia tabaci adults under dual-choice conditions with volatile organic compounds analyzed using solid phase microextraction coupled with gas chromatography-mass spectrometry (GC-MS).Results showed that the presence of CIVIV in chilli plants substantially affects the settling preferences of the B.tabaci,which preferred to settle on noninfected plants.Duration of the egg stage and the longevity and fecundity of adult B.tabaci on CMV-infected chilli plants were not markedly different from those on noninfected chilli plants.In contrast,the developmental time from egg to adult was significantly reduced in CMV-infected chilli plants compared to the noninfected plants.The results also showed that CMV-infected chilli plants released significantly more linalool and phenylacetaldehyde than noninfected plants.Overall,it was suggested that the behavioral response of B.tabaci might be modified by CMV-infected plants,which alter the release of specific headspace volatiles.Based on these results,the modification of plant volatile profiles may help in enhancing the effectiveness of biological control and the protection of crop plants against B.tabaci. | Khalid A.Saad M.N.Mohamad Roff Rebecca H.Hallett Idris B.Abd-Ghani | 2019 | Insect Science2019，26，1： | 1 |
| 12 | 现代化连栋温室彩色甜椒病虫害防治显示文摘<正>彩色甜椒(Capsicum frutescens L),茄科辣椒属,是多种不同果色甜椒的总称,又称为'七彩大椒'。我国在20世纪90年代中期,从荷兰、以色列、美国等地引入,其果形大,果皮光滑,色泽艳丽多彩;口感甜脆,适宜鲜食,营养价值高;采摘时间长,耐低温,耐弱光,同时,彩色甜椒还具有高产、稳产、抗病性强、品质优良、极耐储运等特点。彩色甜椒植株生长旺盛,株高可达2.5~3.0米,成熟后颜色分为乳白、 | 曹华 | 2015 | 北京农业(中旬刊)2015，，7： | 1 |
| 13 | 朝阳地区辣椒丰产栽培技术显示文摘辣椒属被子植物门,双子叶植物纲,茄目,茄科,辣椒属,辣椒种,现在世界各国普遍栽培。辣椒又名番椒、海椒、秦椒、牛角椒、长辣椒、菜椒、辣茄、灯笼椒,拉丁文名:Capsicum annuum L,一年生草本植物。辣椒果实色泽鲜艳,风味好,维生素C的含量丰富,果实通常成圆锥形或长圆形,未成熟时呈绿色,成熟后变成鲜红色、黄色或紫色,以红色最为常见,除鲜食外,还可腌渍和干制,加工成辣椒干、辣椒粉、辣椒油和辣椒酱. | 塔国民 | 2017 | 现代农业2017，，2： | 1 |
| 14 | 温室辣椒整枝栽培经验总结显示文摘辣椒属茄科(Capsicum annuun L)一年生作物)但在热带则为多年生作物。大跃进以来,人民物质生活不断提高,对新鲜蔬菜供应要求更为迫切,长春市二道河子人民公社福利农场在公社党委的正确领导下,社员发挥了敢想敢干的风格,终于试栽成功温室整枝辣椒,给周年供应果菜类提供了有利基础。 | 刘长君 叶启真 张荆丽 | 1960 | 东北农业科学1960，23，5： | 1 |
| 15 | 陕西关中地区线辣椒高产栽培技术显示文摘线辣椒是一年生辣椒种(Capsicum annuum L.)中的一个类型或变种,长期以来,在陕西、甘肃、新疆、四川、重庆、湖南、贵州、云南等省(市、区)有规模化种植。在陕西省关中地区,线辣椒种植历史悠久,自20世纪70年代以来已经形成较大规模,目前,年种植面积稳定在40 000~50 000 hm2,产值22 500~30 000元/h m2,成为当地的一个支柱产业。在陕西这个口味嗜辣的北方省份,辣椒成为了最为朴素的舌尖味道。 | 寇明明 | 2014 | 辣椒杂志2014，，2： | 1 |
| 16 | Pan-plastome approach empowers the assessment of genetic variation in cultivated Capsicum species显示文摘Pepper species(Capsicum spp.)are widely used as food,spice,decoration,and medicine.Despite the recent old-world culinary impact,more than 50 commercially recognized pod types have been recorded worldwide from three taxonomic complexes(A,B,and P).The current study aimed to apply a pan-plastome approach to resolve the plastomic boundaries among those complexes and identify effective loci for the taxonomical resolution and molecular identification of the studied species/varieties.High-resolution pan-plastomes of five species and two varieties were assembled and compared from 321 accessions.Phyloplastomic and network analyses clarified the taxonomic position of the studied species/varieties and revealed a pronounced number of accessions to be the rare and endemic species,C.galapagoense,that were mistakenly labeled as C.annuum var.glabriusculum among others.Similarly,some NCBIdeposited plastomes were clustered differently from their labels.The rpl23-trnI intergenic spacer contained a 44 bp tandem repeat that,in addition to other InDels,was capable of discriminating the investigated Capsicum species/varieties.The rps16-trnQ/rbcL-accD/ycf3-trnS gene set was determined to be sufficiently polymorphic to retrieve the complete phyloplastomic signal among the studied Capsicum spp.The pan-plastome approach was shown to be useful in resolving the taxonomical complexes,settling the incomplete lineage sorting conflict and developing a molecular marker set for Capsicum spp.identification. | Mahmoud Magdy Lijun Ou Huiyang Yu Rong Chen Yuhong Zhou Heba Hassan Bihong Feng Nathan Taitano Esther van der Knaap Xuexiao Zou Feng Li Bo Ouyang | 2019 | Horticulture Research2019，6，1： | 1 |
| 17 | 多效唑对辣椒幼苗生长的影响显示文摘以辣椒幼苗为试材,设置5个浓度梯度,即A(0,对照)、B(50 mg/kg)、C(100 mg/kg)、D(150 mg/kg)和E(200 mg/kg),处理后每10 d取样,验证不同浓度的多效唑浸根处理对植株生长的影响。结果表明:随多效唑浓度提高,辣椒幼苗株高和各部分干重降低,茎粗和根冠比先上升后下降;浓度为100 mg/kg时,壮苗指数最高,是培育壮苗的最适浓度。 | 韩琳 | 2015 | 北京农业(中旬刊)2015，，7： | 1 |
| 18 | Novel structural annotation and functional expression analysis of GTP_EFTU conserved genes in pepper based on the PacBio sequencing data显示文摘Genes containing GTP_EFTU domain mainly express elongation factors(EF),Small GTPases,and GTP-binding proteins,which are closely related to protein synthesis,extension and ATP synthesis.In this study,we identified 39 genes containing GTP_EFTU domains from peppers.The evolutionary trees constructed from capsicum,Arabidopsis,rice,and tomato are mainly divided into 7 subfamilies.Using PacBio(Pacific Biosciences)sequencing and assembly data,we extracted these 39 gene sequences,fromwhich 25 genes had alternative splicing.Particularly,the Capana08g000545 had 16 alternative splicing processes.Accordingly,we performed promoter sequence analysis,subcellular location prediction,the expression analysis of different tissues and periods,and also the GO(Gene ontology)analysis of co-expressed genes.Lastly we did the qRTPCR analysis in 5 stages of pepper fruit development.These analyses revealed important structural and functional information for the identified 39 genes that contain GTP_EFTU domains,providing important references for further follow-up experiments to verify the genes function on plants or their unique roles in peppers. | Bingqian Tang Lingling Xie Xuefeng Li Huiping Yang Yi Liu Xiang Cheng Chenliang Liang Juan Chen Shudong Zhou Duanhua Wang Jingyuan Zheng Xiongze Dai Xuexiao Zou Feng Liu | 2021 | Horticultural Plant Journal2021，7，5： | 1 |
| 19 | Comparative Transcriptome Analysis between Cytoplasmic Male-sterile Line and Its Maintainer During the Floral Bud Development of Pepper显示文摘The male-sterile line has been largely used in the hybrid seed production of pepper, which can effectively improve the efficiency of hybrid seed production. However, the formation mechanism of male sterility in pepper remains unclear. In the present study, we compared the gene expression patterns between pepper cytoplasmic male sterile line 9704 A and its maintainer 9704 B during floral bud development using RNA sequencing technology. A total of 547 976 and 2 416 Differentially Expressed Genes(DEGs) were identified in the stage S1, S2 and S3, respectively,and more than 70% of the DEGs were down-regulated in the sterile line. Gene Ontology(GO), and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were performed to further understand the functions of these identified DEGs. The results showed that the DEGs were mainly enriched in pathways of starch and sucrose metabolism, pentose and glucuronate interconversions. A number of genes, such as MS1, PME5, ATPB, and lots of transcription factors were found down-regulated in the sterile line, and we also identified a series of genes with large differences in expression patterns between sterile line and maintainer line. Collectively, our findings laid a foundation for further molecular breeding in pepper and provided new insights into its mechanism underlying the male sterility. | Junheng Lv Zhoubin Liu Yuhua Liu Lijun Ou Minghua Deng JingWang Jingshuang Song Yanqing Ma Wenchao Chen Zhuqing Zhang Xiongze Dai Xuexiao Zou | 2020 | Horticultural Plant Journal2020，6，2： | 1 |
| 20 | Pun1蛋白的辣椒素合酶活性及其作为辣椒素积累决定因素的证据显示文摘背景:辣椒素类物质,包括辣椒素及其类似物,是导致辣椒果实辛辣的原因。尽管辣椒素为人熟知并且每天都会用到,但是对于辣椒素合成途径中的相关基因并不是完全了解。已有研究表明,p AMT和Pun1编码的蛋白分别催化辣椒素合成途径中的第二步和最后一步反应,并且Pun1编码的蛋白具有辣椒素合酶活性。然而,并没有直接的证据证明Pun1具有辣椒素合酶活性。结果:为了证明Pun1蛋白在辣椒素合成中的作用,我们利用大肠杆菌合成了抗Pun1蛋白抗体,利用该抗体拮抗内源的Pun1活性。同时通过病毒介导的基因沉默技术靶定了Pun1的m RNA,以确认抗Pun1抗体的特异性。在Pun1下调表达的胎座组织中,利用该抗体进行western blot,检测到Pun1蛋白的积累减少,同时辣椒素在胎座中的积累量也降低。从胎座组织中分离得到原生质体,在体外进行辣椒素的从头合成,加入抗Pun1抗体之后,辣椒素的合成受到抑制。通过分析不同辣椒品种的p AMT和Pun1的表达,我们发现辣椒素的高水平积累总是伴随着p AMT和Pun1的高水平表达,也就是说这两个基因对辣椒素合成很重要。比较有辣味辣椒和无辣味辣椒的香草基胺(辣椒素合成的前体物质)和辣椒素的积累水平,结果表明:在辛辣味辣椒中香草基胺的含量很低,推测可能是香草基胺合成之后,Pun1把香草基胺快速转化为辣椒素;而在无辣味辣椒中由于缺乏Pun1,香草基胺的含量积累到很高的水平。结论:对辣椒素从头合成途径和抗Pun1抗体进行原生质体试验,证明了Pun1基因及其产物参与了辣椒素的合成。与辣椒素的积累相比较,分析香草基胺的积累过程,揭示了Pun1是辣椒素和香草基胺积累水平的决定因素。 | Kana Ogawa Katsunori Murota Hanako Shimura Misaki Furuya Yasuko Togawa Takeshi Matsumura Chikara Masuta 马义花 孔秋生 | 2015 | 辣椒杂志2015，，2： | 0 |
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