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| 1 | 钙依赖蛋白激酶(CDPKs)在植物钙信号转导中的作用显示文摘CDPKs在植物钙信号转导中起重要作用。本文介绍了植物钙信号转导及CDPKs的结构与生化性质 ,在此基础上 ,重点总结了CDPKs在植物钙信号转导中的潜在调节作用 ,包括基因表达、代谢、离子和水分的跨膜运输、细胞骨架的动态变化、气孔运动和生长发育等 。 | 刘贯山 陈珈 | 2003 | 植物学通报2003,20,2: | 37 |
| 2 | Calcium-Dependent Protein Kinase CPK21 Functions in Abiotic Stress Response in Arabidopsis thaliana显示文摘钙依赖者蛋白质 kinases (CDPK ) 包括察觉到领域和 kinase 受动器领域的钙在一个分子以内在被联合的植物 serine/threonine 蛋白质 kinases 的一个家庭。到目前为止,在不能生活的应力发信号的生物功能仅仅为很少 CDPK isoforms 被报导了,而为这些 CDPK 的内在的生物化学的机制主要仍然是未知的。这里,我们证明从 Arabidopsis thaliana 的 CPK21 生物化学地响应 hyperosmotic 应力在 vivo 被激活。cpk21 的 Loss-of-function 幼苗对 hyperosmotic 应力更容忍,变异的植物关于标记基因表示和代谢物累积显示出增加的压力回答。在互补在 cpk21 异种背景衬里的转基因的 Arabidopsis,在哪个任何一个 CPK21 野类型,或带氨基酸的替换的全身的酶变体稳定地被表示,压力 responsitivity 被 CPK21 然而并非与 kinase 恢复不活跃的变体。生物化学的描述在 planta,综合并且净化的 CPK21 蛋白质表明在钙绑定领域以内,与 C 终端 EF3 主题和 EF4 主题相比的 N 终端 EF1 主题和 EF2 主题在他们对调整钙的 kinase 活动的贡献不同,为 N 终端 EF 手对建议一个关键角色。我们的数据为在不能生活的应力发信号作出贡献的 CPK21 提供证据并且建议 N 终端 EF 手对是控制 CPK21 的特性的一个察觉到钙的决定因素函数。 | Sandra Franz Britta Ehlert Anja Liese Joachim Kurth Anne-Claire Cazale Tina Romeis | 2011 | Molecular Plant2011,4,1: | 27 |
| 3 | Protein kinases in plant responses to drought, salt,and cold stress显示文摘Protein kinases are major players in various signal transduction pathways. Understanding the molecular mechanisms behind plant responses to biotic and abiotic stresses has become critical for developing and breeding climate-resilient crops. In this review,we summarize recent progress on understanding plant drought, salt, and cold stress responses, with a focus on signal perception and transduction by different protein kinases, especially sucrose nonfermenting1(SNF1)-related protein kinases(Sn RKs),mitogen-activated protein kinase(MAPK) cascades,calcium-dependent protein kinases(CDPKs/CPKs),and receptor-like kinases(RLKs). We also discuss future challenges in these research fields. | Xuexue Chen Yanglin Ding Yongqing Yang Chunpeng Song Baoshan Wang Shuhua Yang Yan Guo Zhizhong Gong | 2021 | Journal of Integrative Plant Biology2021,63,1: | 13 |
| 4 | 钙依赖型蛋白激酶(CDPKs)在植物中的生理功能显示文摘 简要介绍了钙依赖型蛋白激酶(Calcium-dependentProteinKinases,CDPKs)的结构特点及家族成员,重点对CDPKs在植物环境胁迫、防御反应、生长发育、离子通道调节和激素信号等方面的生理功能研究进展进行了综述和讨论,并展望了该领域的研究前景。 | 倪天华 魏幼璋 | 2002 | 西北农林科技大学学报(自然科学版)2002,30,6: | 12 |
| 5 | Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum显示文摘Calcium-dependent protein kinases (CDPKs, EC 2.7.1.37) comprise a large family of Ser/Thr kinases in plants and play an important role in plant Ca2+ signal transduction. A full-length CDPK gene, NtCDPK12 (GenBank accession number GQ337420), was isolated from common tobacco (Nicotiana tabacum) leaves by rapid amplification of cDNA ends (RACE). The NtCDPK12 cDNA is 1 816 bp length and contains an open reading frame (ORF) of 1 461 bp encoding 486 amino acids. Sequence alignments indicated that NtCDPK12 contains all conserved regions found in CDPKs and shows a high level of sequence similarity to many other plant CDPKs. The results of real-time quantitative reverse transcription-PCR (qRT-PCR) showed that NtCDPK12 was highly expressed in stems and increased in roots treated with high-salt or subjected to drought stress, which indicates that NtCDPK12 was induced by high-salt and drought stresses. | CHEN Shuai LIU Guan-shan WANG Yuan-ying SUN Yu-he CHEN Jia | 2011 | Agricultural Sciences in China2011,10,12: | 8 |
| 6 | 钙依赖蛋白激酶(CDPKs)在植物钙信号转导中的生理功能显示文摘介绍了钙依赖蛋白激酶(CDPKs)的结构特点以及酶活性调节,从激素信号、生长发育、细胞骨架、胁迫信号、防御反应、碳氮代谢以及离子通道调节几个方面阐述了CDPKs在植物钙信号转导中的生理功能,并对该领域的研究前景作了展望。 | 雷志华 陈军营 陈新建 | 2007 | 福建林业科技2007,34,3: | 3 |
| 7 | 植物CDPK信号转导途径及其互作组分的功能研究进展显示文摘钙依赖性蛋白激酶(calcium-dependent protein kinase,CDPK或CPK)是植物中最大的钙调蛋白激酶家族,其在植物体内可迅速感受瞬时Ca2+信号的变化,识别并磷酸化特异性底物后,通过信号的级联转导,引发各种生理反应,从而调控植物的生长发育及对多种胁迫的响应.许多植物中均具有CDPKs,根据其氨基酸序列可分为四个家族(Ⅰ-Ⅳ).CDPKs分布广泛且具有不同的亚细胞定位,这使它们能“感知”局部Ca^(2+)浓度变化并与其靶标进行特异性相互作用,从而发挥不同的生物学功能.本文主要介绍了植物CDPK信号转导途径及CDPKs的生物学特性,在此基础上重点讨论了CDPKs的互作组分及其生物学功能. | 周子馨 兰海燕 | 2021 | 新疆大学学报(自然科学版)(中英文)2021,38,6: | 3 |
| 8 | 二穗短柄草钙依赖型蛋白激酶BdCDPK4基因的克隆及其编码蛋白质的生物信息学分析显示文摘二穗短柄草(Brachypodium distachyon L.)因与小麦、大麦等有很高的同源性,作为新型禾本科模式生物吸引了越来越多的研究者。试验采用基因克隆技术分离了二穗短柄草Bd CDPK4基因的编码区序列,并采用生物信息学方法分析了该基因及其编码的蛋白质的序列特征、结构和功能。结果表明,分离了BdCDPK4基因1 851 bp的cDNA序列(Gen Bank登录号为XM_003559516),编码区长度为1 752 bp,编码583个氨基酸,分子量为64.78 ku,等电点为9.12,含有N端可变区、蛋白激酶区、自抑制区、EF手型结构、类似钙调素等结构域。亚细胞定位预测位于叶绿体和液泡上。进化树分析结果显示,该蛋白与小麦Ta CDPK19蛋白的同源性最近。根据各物种间CDPKs蛋白的高同源性,结合小麦TaCDPK19基因被高盐和小麦白粉病菌Blumeria graministritici(Bgt)诱导,推测Bd CDPK4基因也可能参与逆境胁迫下的信号传导途径,在抗逆等生物学过程中发挥重要功能。 | 韦淑亚 赵旭东 王会平 杨广笑 何光源 | 2017 | 浙江农业学报2017,29,2: | 2 |
| 9 | 小麦成熟胚脱分化过程CDPKs基因的差异表达分析显示文摘用Affymetrix小麦基因芯片检测小麦成熟胚脱分化过程中CDPKs基因的表达变化的结果表明,在检测到的16个CDPKs基因中,有15个基因在小麦成熟胚脱分化过程中发生了有意义变化,其中上调表达基因有12个,上调2~7.5倍,下调表达基因有2个,下调3~6.1倍,在不同时点既有上调又有下调的1个。根据已知CDPKs生物功能和它们表达变化趋势以及这些变化趋势与脱分化过程中的吻合程度分析结果,推测CPK2A、CPK2B、CPK6和Os12g0169800基因参与了小麦成熟胚脱分化过程的启动和愈伤组织的形成。 | 雷志华 崔琰 赵一丹 陈军营 陈新建 | 2009 | 植物生理学通讯2009,45,2: | 1 |
| 10 | 植物CDPKs响应逆境胁迫信号传导作用研究进展显示文摘钙依赖蛋白激酶(CDPKs)是由多基因族编码,在植物响应逆境胁迫信号转导通路中处于关键位置。从CDPKs结构特征、CDPKs在非生物胁迫信号转导中的作用、CDPKs在生物胁迫信号转导中的作用和CDPKs介导的信号传导途径中的交互作用这几个方面综合阐述了植物CDPKs响应逆境胁迫信号传导作用研究进展,旨在探讨CDPKs的功能及调控防御机制。 | 韦淑亚 | 2017 | 化工设计通讯2017,43,1: | 0 |
| 11 | Cloning and Expression of Calcium-Dependent Protein Kinase (CDPK) Gene Family in Common Tobacco (Nicotiana tabacum)显示文摘To further study the function of calcium-dependent protein kinase (CDPK) gene family in common tobacco (Nicotiana tabacum),it is necessary to isolate more CDPKs from common tobacco and describe the sequence characteristics,evolutionary relationship and gene expression.Reverse transcription-PCR (RT-PCR),rapid amplification of cDNA (RACE) and bioinformatics methods were used to isolate CDPKs from common tobacco.A phylogenetic tree was created using the MEGA4.0 program and expression patterns of the three full-length CDPK genes were studied by RT-PCR.After all aforementioned efforts,we obtained eight additional common tobacco CDPK genes,of which three possessed complete open reading frames (ORFs).Phylogenetic analysis divided 11 full-length Nicotiana CDPK genes into four subfamilies,and two putative common tobacco and Arabidopsis orthologous CDPK genes might correspond to well-conserved functions.Three full-length CDPK genes in common tobacco were detected in all tobacco organs tested,but their expression patterns were significantly different.Eight non-redundant common tobacco CDPK genes were isolated in this study.Along with the previously characterized CDPK genes,at least 15 members of the CDPK family exist in common tobacco.This work establishes a foundation for a genome-wide study of this important gene family in common tobacco. | TAI Shuai-shuai LIU Guan-shan SUN Yu-he CHEN Jia | 2009 | Agricultural Sciences in China2009,8,12: | 0 |