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| 1 | Detection of HBV DNA and its existence status in liver tissues and peripheral blood lymphocytes from chronic hepatitis B patients显示文摘INTRODUCTIONCumulativeclinicalandexperimentalevidenceindicatesthathostimmuneresponsestohepatitisBvirus(HBV)areresponsibleforl... | TANG Ren Xian, GAO Feng Guang, ZENG Ling Yu, WANG Ying Wei and WANG Yu Long | 1999 | World Journal of Gastroenterology1999，5，4： | 27 |
| 2 | Sequencing of PCR amplified HBV DNA pre-c and c regions in 2.2.15 cells and antiviral action by targeted antisense oligonucleotide directed against sequence显示文摘INTRODUCTIONManystudieshaveshownthatantisenseoligonucleotides(ASONs)canefficientlyinhibitHBVDNAreplicationandexpresioninvitro... | ZHOUG Sen 1, WEN Shou Ming 2, ZHANG Ding Feng 3, WANG Quan Li 4, WANG Seng Qi 4 and REN Hong 3 | 1998 | World Journal of Gastroenterology1998，4，5： | 16 |
| 3 | Current status of gene therapy in gastroenterology显示文摘CurentstatusofgenetherapyingastroenterologyXUChangTai1andPANBoRong2Subjectheadingsgastroenterology;genetherapy;genetransfer... | XU Chang Tai 1 and PAN Bo Rong 2 | 1998 | World Journal of Gastroenterology1998，4，1： | 15 |
| 4 | Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial community structure显示文摘It is unclear whether certain plant species and plant diversity could reduce the impacts of multiple heavy metal pollution on soil microbial structure and soil enzyme activities. Random amplified polymorphic DNA (RAPD) was used to analyze the genetic diversity and microbial similarity in planted and unplanted soil under combined cadmium (Cd) and lead (Pb) pollution. A metal hyper- accumulator, Brassica juncea, and a common plant, Festuca arundinacea Schreb, were used in this research. The results showed that microorganism quantity in planted soil significantly increased, compared with that in unplanted soil with Cd and Pb pollution. The order of microbial community sensitivity in response to Cd and Pb stress was as follows: actinomycetes 〉 bacteria 〉 fungi. Respiration, phosphatase, urease and dehydrogenase activity were significantly inhibited due to Cd and Pb stress. Compared with unplanted soil, planted soils have frequently been reported to have higher rates of microbial activity due to the presence of additional surfaces for microbial colonization and organic compounds released by the plant roots. Two coexisting plants could increase microbe population and the activity of phosphatases, dehydrogenases and, in particular, ureases. Soil enzyme activity was higher in B. juncea phytoremediated soil than in F. arundinacea planted soil in this study. Heavy metal pollution decreased the richness of the soil microbial community, but plant diversity increased DNA sequence diversity and maintained DNA sequence diversity at highlevels. The genetic polymorphism under heavy metal stress was higher in B. juncea phytoremediated soil than in F. arundinacea planted soil. | Yang GAO Chiyuan MIAO Jun XIA Liang MAO Yafeng WANG Pei ZHOU | 2012 | Frontiers of Environmental Science & Engineering2012，6，2： | 14 |
| 5 | Cell-free circulating tumor DNA in cancer显示文摘Cancer is a common cause of death worldwide.Despite significant advances in cancer treatments,the morbidity and mortality are still enormous.Tumor heterogeneity,especially intratumoral heterogeneity,is a significant reason underlying difficulties in tumor treatment and failure of a number of current therapeutic modalities,even of molecularly targeted therapies.The development of a virtually noninvasive 'liquid biopsy' from the blood has been attempted to characterize tumor heterogeneity.This review focuses on cell-free circulating tumor DNA(ctDNA) in the bloodstream as a versatile biomarker.ctDNA analysis is an evolving field with many new methods being developed and optimized to be able to successfully extract and analyze ctDNA,which has vast clinical applications.ctDNA has the potential to accurately genotype the tumor and identify personalized genetic and epigenetic alterations of the entire tumor.In addition,ctDNA has the potential to accurately monitor tumor burden and treatment response,while also being able to monitor minimal residual disease,reducing the need for harmful adjuvant chemotherapy and allowing more rapid detection of relapse.There are still many challenges that need to be overcome prior to this biomarker getting wide adoption in the clinical world,including optimization,standardization,and large multicenter trials. | Zhen Qin Vladimir A.Ljubimov Cuiqi Zhou Yunguang Tong Jimin Liang | 2016 | Chinese Journal of Cancer2016，35，5： | 14 |
| 6 | Expression of liver cancer associated gene HCCA3显示文摘AIM: To study and clone a novel liver cancer reisted gene,and to explore the molecular basis of liver cancer genesis.METHODS: Using mRNA differential display polymerasechain reaction (DDPCR), we investigated the difference of mRNA in human hepatocellular carcinoma (HCC) and paired surrounding liver tissues, and got a gene probe. By screening a human placenta cDNA library and genomic homologous extend, we obtained a full-length cDNA named HCCA3. We analyzed the expression of this novel gene in 42pairs of HCC and the surrounding liver tissues, and distribution in human normal tissues by means of Northern blot assay.RESULTS: A full-length cDNA of liver cancer associated gene HCCA3 has been submitted to the GeneBank nucleotide sequence databases ( Accession No. AF276707 ). The positive expression rate of this gene was 78.6% (33/42) in HCC tissues, and the clinical pathological data showed that the HCCA3 was closely associated with the invasion of tumor capsule ( P = 0.023) and adjacant small metastasis satellite nodules lesions ( P= 0.041). The HCCA3 was widely distributed in the human normal tissues, which was intensively expressed in lungs, brain and colon tissues,while lowly expressed in the liver tissues.CONCLUSION: A novel full-length cDNA was cloned and differentiated, which was highly expressed in liver cancer tissues. The high expression was closely related to the tumor invasiveness and metastasis, that may be the late heredited change in HCC genesis. | Zheng-Xu Wang~1 Gui-Fang Hu~1 Hong-Yang Wang~2 Meng-Chao Wu~2 1 Department of General Surgery,Chinese PEA General Hospital of Lanzhou Military Command,Lanzhou 730050,Gansu Province,China2 Eastern Hepatobilliary Surgical Hospital,Second Military Medical University,Shanghai 200438,China | 2001 | World Journal of Gastroenterology2001，7，6： | 9 |
| 7 | Cloning and expression of NS3 cDNA fragment of HCV genome of Hebei isolate in E.coli显示文摘CloningandexpressionofNS3cDNAfragmentofHCVgenomeofHebeiisolateinE.coliZHUFenLu,LUHaoYing,LIZhuoandQIZhongTianSubjectheadin... | Zhu, FL Lu, HY Li, Z Qi, ZT | 1998 | World Journal of Gastroenterology1998，4，2： | 7 |
| 8 | Hepatitis G virus infection in patients with chronic non-A-E hepatitis显示文摘HepatitisGvirusinfectioninpatientswithchronicnonAEhepatitisCHANGJinHong,WEILai,DUShaoCai,WANGHao,SUNYanandTAOQiMinSubjec... | CHANG Jin Hong, WEI Lai, DU Shao Cai, WANG Hao, SUN Yan and TAO Qi Min | 1997 | World Journal of Gastroenterology1997，3，3： | 7 |
| 9 | Differential expression of a novel colorectal cancer differentiation-related gene in colorectal cancer显示文摘AIM To investigate SBA2 expression in CRC cell lines snd surgical specimens of CRC and sutologous healthy mucosa.METHODS Reverse transcription-polymerase chain reaction (RT-PCR) was used for relative quantification of SBA2 mRNA levels in 4 human CRC cell lines with different grades of differentiation and 30 clinical samples.Normalization of the results was achieved by simultaneous amplification of β-actin as an internal control.RESULTS In the exponential range of amplification, fairly good linearity demonstrated identical amplification efficiency for SBA2 and β-actin (82%). Markedly lower levels of SBA2 mRNA were detectable in tumors, as compared with the coupled normal counterparts ( P < 0.01 ). SBA2 expression was significantly (0.01 < P <0.05) correlated with the grade of differentiation in CRC,with relatively higher levels in well-differentiated samples and lower in poorly-differentiated cases. Of the 9 cases with lymph nodes affected, 78% (7/9) had reduced SBA2 mRNA expression in contrast to 24% (5/21) in nonmetastasis samples (0.01 < P < 0.05 ).CONCLUSION SBA2 gene might be a promising novel biomarker of cell differentiation in colorectal cancer and its biological features need further studies. | Xing-Guo Li Jin-Dan Song Yun-Qing Wang Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical University,Shenyang 110001,Liaoning Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 7 |
| 10 | HBV DNA阳性的中晚期肝癌预后因素分析及抗病毒治疗对生存期的影响显示文摘目的探讨HBV DNA阳性的中晚期原发性肝癌(PHC)预后的影响因素及抗病毒治疗对患者生存的影响。方法选择HBV DNA阳性的93例中晚期肝癌患者临床指标和随访资料,采用Kaplan-Meier法及Cox比例风险回归模型对可能影响其预后的因素进行单变量和多变量回归分析,重点分析病毒载量及核苷(酸)类似物抗病毒治疗对肝癌预后的影响。结果除GGT、Child-Pugh分级、门脉癌栓、治疗方式、治疗次数外,抗病毒治疗也是影响患者预后的重要因素,而病毒载量对预后无统计学意义(P>0.05)。结论虽病毒载量并不影响HBV DNA阳性患者的生存期,但抗病毒治疗仍是影响生存的重要因素,对于HBV DNA阳性的中晚期肝癌应积极抗病毒治疗,以期改善患者的生存。 | 臧允红 丁明权 何登明 | 2011 | 临床肝胆病杂志2011，27，6： | 6 |
| 11 | Detection of bacterial DNA from cholesterol gallstones by nested primers polymerase chain reaction显示文摘DetectionofbacterialDNAfromcholesterolgalstonesbynestedprimerspolymerasechainreactionWUXiaoTing1,XIAOLuJia2,LIXingQuan3an... | WU Xiao Ting 1, XIAO Lu Jia 2, LI Xing Quan 3 and LI Jie Shou 1 | 1998 | World Journal of Gastroenterology1998，4，3： | 6 |
| 12 | Copper transportion of WD protein in hepatocytes from Wilson disease patients in vitro显示文摘AIM: To study the effect of copper transporting P-type ATPase in copper metabolism of hepatocyte and pathogenesis of Wilson disease (WD).METHODS: WD copper transporting properties in some organelles of the cultured hepatocytes were studied from WD patients and normal controls. These cultured hepatocytes were incubated in the media of copper 15mg.L-1 only, copper 15 mg. L-1 with vincristine (agonist of P-type ArPase) 0.5mg. L-1, or copper 15 mg. L-1 withvanadate (antagonist of P-type ATPase) 18.39 mg. L-1separately. Microsome (endoplasmic reticulum and Golgi apparatus), lysosome, mitochondria, and cytosol were isolated by differential centrifugation. Copper contents in these organelles were measured with atomic absorption spectrophotometer, and the influence in copper transportion of these organelles by vanadate and vincristine were comparatively analyzed between WD patients and controls.WD copper transporting P-type ATPase was detected by SDS-PAGE in conjunction with Western blot in liver samples of WD patients and controls.RESULTS: The specific WD proteins (Mr155 000 lanes) were expressed in human hepatocytes, including the control and WD patients. After incubation with medium containing copper for 2 h or 24 h, the microsome copper concentration in WD patients was obviously lower than that of controls,and the addtion of vanadate or vincristine would change the copper transporting of microsomes obviously. When incubated with vincristine, levels of copper in microsome were significantly increased, while incubated with vanadate,the copper concentrations in microsome were obviously decreased. The results indicated that there were Wdproteins, the copper transportion P-type ATPase in the microsome of hepatocytes. WD patients possessed abnormal copper transporting function of WD protein in the microsome, and the agonist might correct the defect of copper transportion by promoting the activity of copper transportion P-type ATPase.CONCLUSION: Copper transportion P-type ATPase plays an important role in hepatocytic copper metabolism.Dysfunction of hepatocytic WD protein copper transportion might be one of the most important factors for WD. | Guo-Qing Hou~1 Xiu-Ling Liang~2 Rong Chen~2 Li-Wen Tang~3 Ying Wang~2 Ping-Yi Xu~2 Ying-Ru Zhang~2 Cui-Hua Ou~2 1 Department of Neurology.Guangzhou First Municipal People’s Hospital,Guangzhou Medical College,Guangzhou 510180,Guangdong Province.China2 Department of Neurology.First Affiliated Hospital.Sun Yat-Sen University of Medical Sciences.Guangzhou 510080.Guangdong Province.China3 Department of Pharmacology,University of Kentucky.Lexington,KY 40506.USA | 2001 | World Journal of Gastroenterology2001，7，6： | 4 |
| 13 | Nonlinear Dynamics in a New Double Chain-Model of DNA显示文摘We investigate the mechanics ofa new double chain-model of DNA. The model consists of two long elastic homogeneous strands (or rods), which represent two polynucleotide chains of the DNA molecule, connected with each other by an elastic membrane (or some linear springs) representing the hydrogen bonds between the base pairs of the two chains. The nonlinear dynamical equations are derived and some solitary wave solutions are discussed. By the way,our model can be regarded as the mechanical model of cubic nonlinear Klein-Gordon equation and 4-field equation. | KONG De-Xing LOU Sen-Yue ZENG Jin | 2001 | Communications in Theoretical Physics2001，，12： | 4 |
| 14 | Analysis of Mitochondrial DNA from the Ancient Tombs of Turfan显示文摘MtDNA was successfully extracted from ten individual bones (femurs) in the tombs of ancient Jushi in Turfan basin, dated back to the year about 3 000-2 500 years ago. By means of four overlapping primers, we got nucleotide sequence of the 218bp length. Ancient mtDNA was analyzed by the sequencing of hypervariable region Ⅰ of the mtDNA control region. The result shows that 9 haplotypes with 24 polymorphic sites were obtained. The phylogenetic analysis indicated that Mongolians and Altai are the population genetically closest to the Jushi groups and Jushi mtDNA pool being an admixture of eastern Asian and European lineages. So our preliminary data imply that an ancient mingling of Euro-Asian population had existed in Turfan basin prior to the early Iron Age. | CUI Yin-qiu 1,2, DUAN Ran-hui 1, LIU Shu-bai 1, JI Chao-neng 2,ZHU Hong 1, LI Wei 1, MAO Yu-min 2 and ZHOU Hui 1,**1. Ancient DNA Laboratory, College of Life Science, Jilin University, Changchun 130023,P. R. China 2. State Key Laboratory of Genetic Engineering, School of Life Science,Fudan University, Shanghai 200433, P. R. China | 2002 | Chemical Research in Chinese Universities2002，18，4： | 3 |
| 15 | DNA and RNA sensor显示文摘This review summarizes recent advances in DNA sensor. Major areas of DNA sensor covered in this review include immobilization methods of DNA, general techniques of DNA detection and application of nanoparticles in DNA sensor. | LIU Tao LIN Lin ZHAO Hong JIANG Long | 2005 | Science China Chemistry2005，48，1： | 3 |
| 16 | HBeAg gene expression with baculovirus vector in silk worm cells显示文摘INTRODUCTIONMiyanoharaetal[1]firstobtainedproductswiththeexpresionofHBeAgactivitybyconstructingayeastexpresionsystem;laterres... | DENG Xiao Zhao, DIAO Zhen Yu, HE Liang, QIAO Ren Liang and ZHANG Lin Yuan | 1999 | World Journal of Gastroenterology1999，5，2： | 3 |
| 17 | Mechanism of intram olecular charge transfer in DNA helix as probed by the use of the fluorescent 2-aminopurine显示文摘As a structural analogue of adenine, 2-aminopurine (2Ap) is often used as a fluorescent probe to study the intramolecular charge transfer reaction in DNA. We have designed and synthesized a series of model DNA helix with the variation in the distance between the 2Ap probe and the GGG sequence, and have investigated, by means of picosecond time-resolved fluorescence spectroscopy, the effect of the length of the bridge (consisting of a number of inosines, I) separating the electron donor (???GGG???) and the acceptor (2Ap) on the charge transfer dynamics. The fluorescence dynamics of 2Ap exhibited three exponential decay components, the one with a time constant of a few hundred picoseconds is assigned to the intramolecular charge transfer from GGG to 2Ap. Within 2.4 nm of the donor-acceptor separation, the rate of charge transfer decreased exponentially upon increasing the separation, from which the decay factor β is determined to be 1.3 nrrf-1. Beyond 2.4 nm, however, the rate started toincrease, this abnormal behavior of charge transfer is interpreted in terms of the match of elec- tronic energies between the l-bridge and the donor/acceptor couple. | ZHANG Huijuan WANG Peng WANG Xuefei FENG Juan XU Sichuan AI Xicheng ZHANG Xingkang ZHANG Jianping | 2004 | Science China Chemistry2004，47，5： | 3 |
| 18 | 乙型肝炎病毒耐药基因测序检测性能验证显示文摘目的:探讨在ISO15189以及美国病理学家协会( CAP)实验室认可体系下,建立使用一代测序(Sanger测序法)进行乙型肝炎病毒(HBV)耐药基因检测的项目性能验证标准。方法方法学建立。2012年8至12月收集复旦大学附属华山医院肝炎门诊及住院患者中临床表现HBV耐药患者25例。从测定下限、精密度、正确性、分析特异性、可报告范围/参考范围等方面进行性能评估。测序质量的验证基于整体测序图谱的荧光信号值、性噪比、测序峰图、QV值等评估参数进行。结果可检测出正常背景下10%~20%的突变;有较好的精密度;正确性100%;未发现有明显干扰及交叉污染。结论测序方法的性能验证要结合实际应用。特别是测序分析的质量评估需要针对不同的检测目的以及检测对象建立相关标准并适当进行调整以符合临床需要。本试验方法检测乙型肝炎耐药基因可应用于临床检测。 | 胡婷婷 陈宇明 关明 刘维薇 | 2014 | 中华检验医学杂志2014，37，10： | 2 |
| 19 | Preparation of PLL-PEG-PLL and its application to DNA encapsulation显示文摘PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aque- ous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10―35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9. | YANG Zigang1, ZHOU Fen1, YUAN Jianjun1, MA Lixin2, ZHAI Chao2 & CHENG Shiyuan1 1. School of Chemistry and Materials Science, Hubei University, Wuhan 430062, China 2. School of Life Science, Hubei University, Wuhan 430062, China | 2006 | Science China Chemistry2006，49，4： | 1 |
| 20 | Serum deprivation enhances DNA synthesis of human hepatoma SMMC-7721 cells显示文摘SerumdeprivationenhancesDNAsynthesisofhumanhepatomaSMMC7721celsJIANGShiMingandXUZhaoHuiSubjectheadingsliverneoplasms;carc... | Jiang, SM Xu, ZH | 1998 | World Journal of Gastroenterology1998，4，2： | 1 |
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