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| 1 | NIR-II ?uorescence in vivo confocal microscopy with aggregation-induced emission dots显示文摘Significantly reduced tissue scattering of fluorescence signals in the second near-infrared(NIR-Ⅱ,1,000–1,700 nm)spectral region offers opportunities for large-depth in vivo bioimaging.Nowadays,most reported works concerning NIR-II fluorescence in vivo bioimaging are realized by wide-field illumination and 2D-arrayed detection(e.g.,via InGaAs camera),which has high temporal resolution but limited spatial resolution due to out-of-focus signals.Combining NIR-II fluorescence imaging with confocal microscopy is a good approach to achieve high-spatial resolution visualization of biosamples even at deep tissues.In this presented work,a NIR-II fluorescence confocal microscopic system was setup.By using a kind of aggregation-induced emission(AIE)dots as NIR-II fluorescent probes,800 lm-deep 3D in vivo cerebrovascular imaging of a mouse was obtained,and the spatial resolution at 700 lm depth could reach 8.78 lm.Moreover,the time-correlated single photon counting(TCSPC)technique and femtosecond laser excitation were introduced into NIR-II fluorescence confocal microscopy,and in vivo confocal NIR-II fluorescence lifetime microscopic imaging(FLIM)of mouse cerebral vasculature was successfully realized. | Wenbin Yu Bing Guo Hequn Zhang Jing Zhou Xiaoming Yu Liang Zhu Dingwei Xue Wen Liu Xianhe Sun Jun Qian | 2019 | Science Bulletin2019，64，6： | 10 |
| 2 | 荧光寿命成像显微技术显示文摘介绍荧光寿命成像显微(FLIM)技术及其在生物物理、生物化学及临床医学诊断等领域的最新研究成果和发展现状,并就其未来的发展及应用研究进行了讨论。 | 屈军乐 牛憨笨 郭宝平 | 1997 | 应用激光1997，17，3： | 5 |
| 3 | A two-photon mitotracker based on a naphthalimide fluorophore:Synthesis, photophysical properties and cell imaging显示文摘PAHPN, a naphthalimide-based mitotracker with reasonable two-photon excitation emission activity and polarity-sensitive fluorescence properties has been efficiently synthesized and studied in twophoton, co-localization, and FLIM imaging. | Yong Dai Bao-Kuo Lv Xin-Fu Zhang Yi Xiao | 2014 | Chinese Chemical Letters2014，25，7： | 3 |
| 4 | 荧光寿命成像显微的频域零差法测量及数据处理显示文摘介绍了一种实现荧光寿命成像显微技术(FLIM)的频域零差法,并改进了测量数据的逐点分析法,设计了相应的数据处理软件.通过对模拟数据的处理表明,完全满足FLIM数据处理的要求。 | 屈军乐 牛憨笨 | 1997 | 应用激光1997，17，5： | 3 |
| 5 | 茎瘤固氮根瘤菌ORS571鞭毛马达基因fliN与fliM的功能分析显示文摘【目的】考察茎瘤固氮根瘤菌ORS571中鞭毛马达蛋白FliN、FliM的编码基因分别缺失的突变体表型,初步探究其功能机理。【方法】本研究采用同源重组和三亲本接合转移的方法构建突变体,测定野生型及突变株的生长曲线、趋化性、胞外多糖的分泌、生物膜的形成及细胞絮凝等表型。【结果】三种菌株的生长速率基本无差,与野生型菌株相比突变株鞭毛结构丧失,趋化能力、分泌的胞外多糖和生物膜形成能力均下降,但相同时间内细胞絮凝程度比野生型明显。【结论】实验表明,鞭毛基因fliN、fliM对茎瘤固氮根瘤菌ORS571鞭毛的形成、趋化运动、胞外多糖的分泌、生物膜的形成及细胞絮凝能力等均有调控作用。 | 沈日敏 刘卫 孙雨 李润植 解志红 | 2018 | 微生物学报2018，58，5： | 2 |
| 6 | Monitoring cellular metabolism of 3T3 upon wild type E. coli infection by mapping NADH with FLIM显示文摘Fluorescence lifetime imaging microscopy (FLIM) has gained popularity as a sensitive technique to monitor the functional/conformational states of reduced nicotinamide adenine dinucleotide (NADH), one of the main compounds of oxidative phosphorylation. In this letter, we apply the technique to characterize the metabolic changes in mouse embryonic fibroblast 3T3 cells upon bacterial infection. A gradual shortening of the decaying time constants in both the short and the long lifetime components of NADH’s autofluorescence is detected. The ratio of the short and the long lifetime components’ relative contributions, however, shows a rapid increase, indicating the rise of cellular metabolic activity over the course of infection. | Tatyana Buryakina Pin-Tzu Su Vladimir Gukassyan Wan-Jr Syu Fu-Jen Kao | 2010 | Chinese Optics Letters2010，8，10： | 0 |
| 7 | Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications显示文摘Inorganic quantum dots(QDs)have excellent optical properties,such as high°uorescence intensity,excellent photostability and tunable emission wavelength,etc.,facilitating them to be used as labels and probes for bioimaging.In this study,CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope(FLIM)and stimulated emission depletion(STED)nanoscopy imaging.The emission peak of CdSe@ZnS QDs centered at 526 nm with a narrow width of 19 nm and the photoluminescence quantum yield(PLQY)was 64%.The QDs presented excellent anti-photobleaching property which can be irradiated for 400 min by STED laser with 39.8 mW.The lateral resolution of 42.0 nm is demonstrated for single QDs under STED laser(27.5 mW)irradiation.Furthermore,the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5 nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD probes.Meanwhile,Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM,and their fluorescence lifetime was around 3.1 ns,consistent with the in vitro value,suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments. | Mengjie Zhao Shuai Ye Xiao Peng Jun Song Junle Qu | 2019 | Journal of Innovative Optical Health Sciences2019，，5： | 0 |
| 8 | An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays显示文摘Fluorescence lifetime imaging(FLIM)is increasingly used to read out cellular autofluorescenceoriginating from the coenzyme NADH in the context of investigating cell metabolic state.Wepresent here an automated multiwell plate reading FLIM microscope optimized for UV illumi-nation with the goal of extending high content fluorescence lifetime asays to readouts ofmetabolism,We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues associated with its implementation.In particular,weillustrate its capability to read out the NADH-lifetime response of cells to metabolic modulators,thereby illustrating the potential of the instrument for cytotoxicity studies,assays for drugdiscovery and stratified medicine. | Douglas J.Kelly Sean C.Warren Sunil Kumar Joao L.Lagarto Benjamin T.Dyer Anca Margineanu Eric W.-F.Lam Chris Dunsby Paul M.W.French | 2014 | Journal of Innovative Optical Health Sciences2014，7，5： | 0 |
| 9 | Deep tissue imaging by enhanced photon collection显示文摘We have developed a two-photon fluorescence microscope capable of imaging up to 4mm inturbid media with micron resolution.The key feature of this instrument is the innovative de-tector,capable of collecting emission photons from a wider surface area of the sample thandetectors in traditional two-photon microscopes.This detection scheme is extremely efficient inthe collection of emitted photons scattered by turbid media which allows eight fold increase in theimaging depth when compared with conventional two-photon microscopes.Furthermore,thissystem also has in-depth fluorescence lifetime imaging microscopy(FLiM)imaging capabilitywhich increases image contrast.The detection scheme captures emission light in a transmissionconfiguration,making it extremely efficient for the detection of second harmonic generation(SHG)signals,which is generally forward propagating.Here we present imaging experiments oftissue phantoms and in vivo and ea vivo biological tissue performed with this microscope. | Viera Crosignani Sohail Jahid Alexander Dvornikov Enrico Gratton | 2014 | Journal of Innovative Optical Health Sciences2014，7，5： | 0 |
| 10 | Forthrightly monitoring ferroptosis induced by endoplasmic reticulum stresses through fluorescence lifetime imaging of microviscosity increases with a specific rotor显示文摘To test the hypothesis that the microviscosity changes of Endoplasmic Reticulum(ER)can be a useful indicator of ferroptosis promoted by ER Stresses(ERS),a new ER targeting viscosity rotor,L-Vis-1 was developed and applied in the quantitation of viscosity by FLIM imaging in live cells.The FLIM imag-ing exhibited an excellent resolution almost as good as the corresponding confocal imaging,more sig-nificantly,during ferroptosis processes promoted by different types of ERS,the viscosity increases were clearly monitored by FLIM of L-Vis-1 within ER,which has not been demonstrated before. | Chuanhao Liu Lin Zhou Lijuan Xie Ying Zheng Huizi Man Yi Xiao | 2022 | Chinese Chemical Letters2022，33，5： | 0 |
| 11 | Evaluating the tumor stratification with a lysosomal pH sensitive-probe by fluorescence lifetime imaging显示文摘Owing to the anaerobic metabolism in the tumor,abundant acidic metabolites are produced and accumulated in the cells.Therefore,the cells in different tumor layers are directly linked to the pH micro-environment.Nevertheless,due to the lack of robust tools,the high-efficient evaluation of the acidic micro-environment of tumor stratification faces the challenge of accurate diagnosis.We designed a new pH sensitive fluorescent lifetime probe target to lysosomes.As we expected,the fluorescence lifetime of PLN possesses a good linear fit to the pH value,which could detect the pH change at a single lysosome level in real time,and then evaluate the different acidity of tumor stratification.The probe PLN is successfully used to evaluate the tumor stratification by fluorescence lifetime imaging microscopy(FLIM)for the first time,which is of great significance in the preoperative diagnosis of clinical tumor treatment or evaluation of drug delivery effect. | Jun Yan Xing Liang Qian Zhang Luolin Wang Weiying Lin | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
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