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| 1 | High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay显示文摘Viruses are extremely heterogeneous entities; the size and the nature of their genetic information, as well as the strategies employed to amplify and propagate their genomes, are highly variable. However, as obligatory intracellular parasites, replication of all viruses relies on the host cell. Having co-evolved with their host for several million years, viruses have developed very sophisticated strategies to hijack cellular factors that promote virus uptake, replication, and spread. Identification of host cell factors(HCFs) required for these processes is a major challenge for researchers, but it enables the identification of new, highly selective targets for anti viral therapeutics. To this end, the establishment of platforms enabling genome-wide high-throughput RNA interference(HT-RNAi) screens has led to the identification of several key factors involved in the viral lifecycle. A number of genome-wide HT-RNAi screens have been performed for major human pathogens. These studies enable first inter-viral comparisons related to HCF requirements. Although several cellular functions appear to be uniformly required for the life cycle of most viruses tested(such as the proteasome and the Golgi-mediated secretory pathways), some factors, like the lipid kinase Phosphatidylinositol 4-kinase Ⅲα in the case of hepatitis C virus, are selectively required for individual viruses. However, despite the amount of data available, we are still far away from a comprehensive understanding of the interplay between viruses and host factors. Major limitations towards this goal are the low sensitivity and specificity of such screens, resulting in limited overlap between different screens performed with the same virus. This review focuses on how statistical and bioinformatic analysis methods applied to HTRNAi screens can help overcoming these issues thus increasing the reliability and impact of such studies. | Sandeep Amberkar Narsis A Kiani Ralf Bartenschlager Gualtiero Alvisi Lars Kaderali | 2013 | World Journal of Virology2013，2，2： | 9 |
| 2 | Association of HER2 status with prognosis in gastric cancer patients undergoing R0 resection: A large-scale multicenter study in China显示文摘AIM: To determine whether the positive status of human epidermal growth receptor 2(HER2) can be regarded as an effective prognostic factor for patients with gastric cancer(GC) undergoing R0 resection.METHODS: A total of 1562 GC patients treated by R0 resection were recruited. HER2 status was evaluated in surgically resected samples of all the patients using immunohistochemical(IHC) staining. Correlations between HER2 status and clinicopathological characteristics were retrospective analyzed. Hazard ratios(HRs) and 95% confidence intervals(CIs) were estimated using Cox proportional hazard model, stratified by age, gender, tumor location and tumor-nodemetastasis(TNM) stage, with additional adjustment for potential prognostic factors.RESULTS: Among 1562 patients, 548(positive rate = 35.08%, 95%CI: 32.72%-37.45%) were HER2 positive. Positive status of HER2 was significantly correlated with gender(P = 0.004), minority(P < 0.001), tumor location(P = 0.001), pathological grade(P < 0.001), TNM stage(P < 0.001) and adjuvant radiotherapy(74.67% vs 23.53%, P = 0.011). No significant associations were observed between HER2 status and disease free survival(HR = 0.19, 95%CI: 0.96-1.46, P = 0.105) or overall survival(HR = 1.19, 95%CI: 0.96-1.48, P = 0.118) using multivariate analysis, although stratified analyses showed marginally statistically significant associations both in disease free survival and overall survival, especially among patients aged < 60 years or with early TNM stages(Ⅰ and Ⅱ). Categorical age, TNM stage, neural invasion, and adjuvant chemotherapy were, as expected, independent prognostic factors for both disease free survival and overall survival. CONCLUSION: The positive status of HER2 based on IHC staining was not related to the survival in patients with GC among the Chinese population. | Guo-Shuang Shen Jiu-Da Zhao Jun-Hui Zhao Xin-Fu Ma Feng Du Jie Kan Fa-Xiang Ji Fei Ma Fang-Chao Zheng Zi-Yi Wang Bing-He Xu | 2016 | World Journal of Gastroenterology2016，22，23： | 8 |
| 3 | 鄂温克族与鄂伦春族的群体遗传学研究(续)显示文摘本次调查采印了645名鄂温克族和424名鄂伦春族中、小学生和部分牧民、猎民的指纹和掌纹。观察了各型指纹出现率、指纹总嵴数、atd角和掌褶纹等指标。 1.各型指纹出现率 弓(A)、尺侧箕(L^u)、桡侧箕(L^r)和斗(W)的出现频率见表11。额左旗和陈旗鄂温克人中斗多于箕,与汉族相似。有些指纹型在某个手指上较为多见。 | 陈良忠 杜若甫 | 1983 | 人类学学报1983，2，4： | 3 |
| 4 | 人类影响气候研究的一个新观点:人口迁移流动的作用显示文摘传统上,人类影响气候的研究主要针对温室气体与气溶胶排放、土地利用等人类活动的特定方面.例如,IPCC第五次评估报告将20世纪50年代以来全球平均地表温度增加主要归因于人类活动的影响;1951~2010年温室气体引起的增温可能为0.5~1.3℃,气溶胶等其他人为强迫的影响可能为-0.6~0.1℃[1].最近几十年,大规模人口从乡村迁移到城市. | 张井勇 吴凌云 | 2016 | 科学通报2016，61，15： | 2 |
| 5 | Correlates of extinction risk in Chinese endemic birds显示文摘Background: China has a relative high degree of endemism of birds due to its large area, diversified topography, and varied climates and habitats. Among the 77 Chinese endemic birds, 29 species are classified as threatened according to the officially released China Biodiversity Red List in 2015. Chinese endemic birds should be the focus of conservation because their local extinction in China means complete global extinction. However, to date, no study has explicitly examined the patterns and processes of extinction and threat in Chinese endemic birds. Methods: We obtained eleven biological traits and four extrinsic factors that are commonly hypothesized to influence extinction risk. After phylogenetic correction, these factors were used separately and in combination to assess their associations with extinction risk. Results: We found that 37.7% of Chinese endemic birds were listed as threatened (Vulnerable, Endangered and Critically Endangered). Small range size, high hunting vulnerability, and high human population density were important predictors of high extinction risk in Chinese endemic birds. Conclusions: Our study is the first to systematically investigate the patterns and processes of extinction risk in Chinese endemic birds. We suggest that endemic species with small range size and living in area with high human densities require conservation priorities. Conservation efforts should also focus on the reduction of human threats, such as human hunting and habitat degradation, for the effective preservation of Chinese endemic birds. | Chuanwu Chen Di Zeng Yuhao Zhao Yiru Wu Junfeng Xu Yanping Wang | 2019 | Avian Research2019，10，2： | 1 |
| 6 | Sero-Epidemiological Survey of Crimean-Congo Hemorrhagic Fever among the Human Population of the Punjab Province in Pakistan显示文摘Dear Editor,Crimean-Congo hemorrhagic fever(CCHF),caused by the CCHF virus(CCHFV),is a tick-borne zoonotic infection characterized by myalgia,high-grade fever(>38℃),headache,nausea,bleeding from the body cavities,and in 10%–50%of cases,results in death(Swanepoel et al.1989).As CCHFV belongs to the Nairoviridae family,the virus can be transmitted to humans through the bite of infected ticks or by contact with the tissues or blood of infected animals(Bente et al.2013). | Muhammad Furqan Shahid Muhammad Zubair Shabbir Kamran Ashraf Muzaffar Ali Saima Yaqub Aziz Ul-Rahman Nageen Sardar Nadia Mukhtar Zarfishan Tahir Tahir Yaqub | 2020 | Virologica Sinica2020，35，4： | 0 |
| 7 | The Study of Human Resource Development for Chinese Old Generation In the Context of the Aging Population显示文摘 | Yiyi Xu | 2015 | International English Education Research2015，，7： | 0 |
| 8 | Pondering over Several Problems in Disaster Research | SHAN Xiuzheng (Seismological Bureau of Shaanxi Province, Xi’an 710068) | 2001 | Natural Disaster Reduction in China2001，，2： | 0 |
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