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| 1 | Function of apoptosis and expression of the proteins Bcl-2,p53 and C-myc in the development of gastric cancer显示文摘INTRODUCTIONIn China ,the incidence and mortality of gastric cancer rank the second among all cancers. Recent development of cancer [1-20].The aim of this study was investigat the insight of apoptosis and bcl-2, p53 and C-myc protein expression in the development of gastric cancer . | An Gao Xu Shao Guang Li Ji Hong Liu Ai Hua Gan Research Laboratory of Digestive Disease,Huizhou Central People’s Hospital,Huizhou 516001,Guangdong Province,ChinaDr.An Gao Xu graduated from Guangdong Medical College in 1984.He is an associate physician-in-chief,specializing in the research and treatment of gastrointestinal and liver tumors.He has published 24 papers and 1 book. | 2001 | World Journal of Gastroenterology2001，7，3： | 91 |
| 2 | Immunohistochemical study on p53,H-rasp21,c-erbB-2 protein and PCNA expression in HCC tissues of Hart and minority ethnic patients显示文摘AIM To find out the difference of humanprimary liver carcinogenesis between Han andminority ethnic patients in Xinjiang.METHODS Expression of p53,c-erbB-2,H-rasp21 protein and proliferating cell nuclearantigen(PCNA)in tumor tissues of 50 patients(Han 38,minority 12)with primary hepaticcarcinoma was detected byimmunohistochemistry(LSAB).RESULTS The positive frequency of p53,c-erbB-2,H-rasp21 and PCNA expression was46.0%(23/50),70.0%(35/50),68.0%(34/50)and 82.0%(41/50)in tumor tissues;4.0%(2/50),22.0%(11/50),64.0%(32/50)and 52.0%(26/ 50)in peritumors respectively and asignificant difference,except for H-rasp21,ofoncogene alteration was found(P<0.05)between tumor and non-tumorous tissues.Combined the three oncogenes alteration,26%(13/50)tumor tissues had positiveimmunoreactivity,but in peritumor and normallivers it was negative.The positive rate of p53,c-erbB-2 and H-rasp21 protein expression was39.5%(15/38),60.5%(23/38)and 39.5%(15/38)in tumors of Han patients;66.7%(8/12),100%(12/12)and 75.0%(9/12)in minoritiesrespectively,with statistical difference (P<0.05).CONCLUSION Overexpression of p53,c-erbB-2and H-rasp21 in human primary liver carcinoma isan important biomarker of genetic alteration.The different frequency of these oncogeneticchanges may reflect some environmental or/andethnic hereditary factors affecting the livercarcinogenesis.The special life style of Han,Uygur,Kazak and Mongolia nationalities inXinjiang may also be related to theetiopathogenesis of this disease. | Guo Yue Lin Zhao Lun Chen Cai Mo Lu Ying Li Xiao Jia Ping Rong Huang Department of Laboratory Medicine,Chinese PLA 474 Hospital,Urumqi 830011,Xinjiang Uygur Autonomic Region,China Department of Pathology,the 1st Teaching Hospital,Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomic Region,China | 2000 | World Journal of Gastroenterology2000，6，2： | 40 |
| 3 | Chemoprevention of tea on colorectal cancer induced by dimethylhydrazine in Wistar rats显示文摘AIM To investigate the chemopreventiveeffects of green tea and tea pigment on 1,2-dimethylhydrazine(DMH)-induced rat colorectalcarcinogenesis.METHODS Male weaning Wistar rats wererandomly allocated into four groups.Rats in thepositive control group were given s.c.injectionof DMH,once a week for ten weeks;rats in tea-treated groups,with the same DMH treatment asin the positive group,received 2% green tea and0.1% tea pigments;rats in the negative controlgroup were given s.c.injection of the samevolume of saline as well as DMH in the positivegroup.Animals were sacrified and necropsied atthe end of week 16 and week 32.RESULTS Aberrant cryptic foci(ACF)wereformed in animals in DMH-treated groups at theend of week 16.Compared to the DMH group,green tea and tea pigments groups had less ACF(148.25 and 204.25,respectively,P<0.01).Atthe end of week 32,all rats in DMH groupdeveloped large intestinal tumors.The resultsalso showed that DMH increased labeling index(LI)of proliferating cell nuclear antigen(PCNA)of intestinal mucosa and the expression of ras-p21.However,in the tea-treated groups,PCNA-LI was significantly reduced as compared withthe positive control group(36.63 and 40.36 inthe green tea group and tea pigment group,respectively,at the end of the experiment,P<0.01).ras-p21 expression was alsosignificantly reduced(2.07 and 2.36 in the colontumors of rats in the green tea group and teapigments group,respectively at the end of theexperiment,P<0.01).Furthermore,green tea and tea pigment inhibited the expression of Bcl-2protein(2,5,1,0 and 2,4,1,0,respectively,at the end of the experiment P<0.01),andinduced expression of Bax protein(0,1,3,4and 0,1,4,3,respectively,P<0.01).CONCLUSION Chinese green tea drinkinginhibited ACF and colonic tumors formation inrats,which showed that tea had a significantchemopreventive effect on DMH-inducedcolorectal carcinogenesis.Such effects may bedue to suppression of cell proliferation andinduction of apoptosis in the intestinal crypts. | Jia XD Han C | 2000 | World Journal of Gastroenterology2000，6，5： | 21 |
| 4 | The expression of c-kit and proliferating cell nuclear antigen in oval cells of rats with hepatocellular carcinoma显示文摘OBJECTIVE: To study the relationship between oval cells and primary hepatocarcinoma and theexpression of c-kit and proliferating cell nuclear antigen (PCNA) in oval cells of rats with hepatocellularcarcinoma.METHODS: A hundred and twenty clean SD rats were divided into three groups: normal group,cancer-induction group and intervention group. The normal group was fed with standard forage while therest two groups were fed with 3’-methyl-2-methylamino-azobenzene (DAB) to induce carcinoma for 14weeks and then fed with standard forage and water. Uscharidin was injected abdominally to theintervention group from the first week to the 14th week. All rats were killed and biopsy specimens weretaken from the left and right liver lobes for immunohistochemical staining of c-kit and PCNA on the 2nd,4th, 6th, 8th, 10th, 12th, 14th, 16th, 18th, 20th, 22nd, and 24th week.RESULTS: From the 2nd to 14th week after liver infection, c-kit positive cells, mainly oval cells werefound in the portal area in the carcinoma-induction group and dotted positive pigmentations in liverlobules. In the 22nd week, a large number of cancerous nodes occurred and nuclei heteromorphi-m wasapparent; the number of positive cell decreased but positive cells could be sparsely observed in cancerousnodes. In the 2nd week of the carcinoma-induction process, PCNA positive cells were oval cells in theportal area. In the 4th week, a lot of hepatic cells were positively stained, especially in the central veinarea. In the 6th week, PCNA positive cells could be seen in the lobules of the liver. In the 8th week, thenumber of PCNA cells decreased comparatively. From the 10th to 14th week, oval cells in the portal areawere still over-expressed. From the 16th to 24th week, a large number of cancerous nodes occurred andPCNA was over-expressed in some of them. In necrotic cancerous nodes, the para-cancerous PCNApositive cells were sparsely distributed and their number was less than that of PCNA positive cells ofcancerous tissues.CONCLUSIONS: Hepatic stem cells originating from the terminal biliary plexus of the portal area areinvolved in the development of hepatocarcinoma because c-kit positive cells expressed in cancerousnodes, accompany the whole process of the development. In the middle inflammatory period ofcarcinoma-induction, the expression of PCNA in hepatic cells peaked, but the index decreased in the lateinflammatory period and in the proliferated fibrosis stage. The expression of PCNA is a tortuous process,going up, down, then up again from normal tissues to cancerous tissues. Combined with pathologicalfindings, PCNA can be considered as a warning index for carcinomatous cells. | Chi-Hua Fang Wei Zhang Xin-Yong Zhu Jia-Qing Gong Gang-Qing Zhang the Department of Hepatobiliary Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，4： | 7 |
| 5 | A comparative study on proliferating activity between HBV-related and HCV-related small HCC显示文摘AcomparativestudyonproliferatingactivitybetweenHBVrelatedandHCVrelatedsmalHCCYUShaoJunSubjectheadingsliverneoplasms;carci... | YU ShaoJun | 1997 | World Journal of Gastroenterology1997，3，4： | 7 |
| 6 | The Relationship between Apoptosis and the Expression of Proliferating Cell Nuclear Antigen and the Clinical Stages in Gastric Carcinoma显示文摘The relationship between the apoptosis and the expression of proliferating cell nuclear antigen (PCNA) and the clinical stages in gastric cancers was studied. By using terminal deoxynucleotidyl transferase mediated nick end labelling (TUNEL) technique and PCNA immunohistochemical staining, the apoptosis and the expression of PCNA in tissue of gastric carcinoma were assayed in situ, the index of apoptosis (AI), index of PCNA (PI) and the rate of AI/PI were calculated. AI and PI in gastric cancer tissues were (6.5±3.7) % and (49.8±15.9) % respectively, and the rate of AI/PI was 0.13±0.05, which were obviously different from those of normal gastric mucosa in paragastric cancer ( P <0.01). With the advanced TNM stages of gastric carcinoma, the AI was decreased, PI was increased and the rate of AI/PI decreased in gastric carcinoma. There was significant difference in them between the gastric cancer tissues and normal gastric mucosa in pericarcinoma in TNM stage Ⅱ to Ⅳ ( P <0.05). It was suggested that the decreased apoptotic cells and the increased proliferating cells were obviously related to the tumor genesis and tumor progression in gastric carcinoma. The AI, PI and the rate of AI/PI would become the prognostic factors in advanced gastric carcinoma. | 陶凯雄 陈道达 田源 卢晓明 杨秀萍 | 2000 | Journal of Huazhong University of Science and Technology(Medical Sciences)2000，20，3： | 7 |
| 7 | Appearance of an inhibitory cell nuclear antigen in rat and human serum during variable degrees of hepatic regenerative activity显示文摘METHODSInanimalstudies,adultmaleSpragueDawleyrats(n=3-4/group)weresacrificedat0,12,24,36,48,72and96hoursfollowing70%partia... | N Assy 1,2 ,YW Gong 3,M Zhang 3 and GY Minuk 3,4 | 1999 | World Journal of Gastroenterology1999，5，2： | 6 |
| 8 | Influence of norcantharidin on proliferation,proliferation-related gene proteins prolifera-ting cell nuclear antigen and Ki-67 of human gallbladder carcinoma GBC-SD cells显示文摘BACKGROUND: Gallbladder carcinoma is a highly lethal and aggressive disease with early metastasis, strong invasion and poor prognosis. Most patients with this disease are at the advanced and un-resectable stage and should be consi- dered for palliative treatment such as chemotherapy and ra- diotherapy. Unfortunately, reports of chemotherapy and radiotherapy for gallbladder carcinoma are disappointing. We investigated the influence of norcantharidin (NCTD) on proliferation, proliferation-related gene proteins PCNA and Ki-67 of human gallbladder carcinoma GBC-SD cells in vitro. METHODS: GBC-SD cell lines of human gallbladder carci- noma were cultured by the cell culture technique. The ex- periment was divided into NCTD group and control group. The tetrazolium-based colorimetric assay was used to evaluate cell growth. The streptavidin-biotin complex method was used to determine the expressions of prolifera- tion-related gene proteins PCNA and Ki-67 of human gall- bladder carcinoma GBC-SD cells. RESULTS: NCTD inhibited the growth and proliferation of GBC-SD cells from 10 mg/L or after 6 hours in a dose- and time-dependent manner, with the IC50 value of 56.18 μg/ ml at 48 hours. After treatment with NCTD, the expression of PCNA (0.932 ±0.031 vs. 0.318 ±0.023, P<0.001) and Ki-67 (0.964 ±0.092 vs. 0.297 ±0.018, P<0.001) proteins were decreased significantly. CONCLUSION: NCTD inhibits the proliferation of human gallbladder carcinoma GBC-SD cells in vitro and the expres- sion of their proliferation-related gene proteins PCNA and Ki-67. | Yue-Zu Fan, Jin-Ye Fu, Ze-Ming Zhao and Cun-Qiu Chen Shanghai, China Department of Surgery, Tongji Hospital of Tongji U- mversity, Shanghai 200065, China Department of Surgery, Pudong People’ s Hospital, Shanghai 201200 , Chi- na | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 5 |
| 9 | Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease显示文摘AIM: To establish a mouse model of alcohol-driven hepatocellular carcinoma(HCC) that develops in livers with alcoholic liver disease(ALD).METHODS: Adult C57BL/6 male mice received multiple doses of chemical carcinogen diethyl nitrosamine(DEN) followed by 7 wk of 4% Lieber-De Carli diet. Serum alanine aminotransferase(ALT), alpha fetoprotein(AFP) and liver Cyp2e1 were assessed. Expression of F4/80, CD68 for macrophages and Ly6 G, MPO, E-selectin for neutrophils was measured. Macrophage polarization was determined by IL-1β/i NOS(M1) and Arg-1/IL-10/CD163/CD206(M2) expression. Liver steatosis and fibrosis were measured by oil-red-O and Sirius red staining respectively. HCC development was monitored by magnetic resonance imaging, confirmed by histology. Cellular proliferation was assessed by proliferating cell nuclear antigen(PCNA).RESULTS: Alcohol-DEN mice showed higher ALTs than pair fed- DEN mice throughout the alcohol feeding without weight gain. Alcohol feeding resulted in increased ALT, liver steatosis and inflammation compared to pair-fed controls. Alcohol-DEN mice had reduced steatosis and increased fibrosis indicatingadvanced liver disease. Molecular characterization showed high estlevels of both neutrophil and macrophage markers in alcohol-DEN livers. Importantly, M 2 macrophages were edominantly higher in alcohol-DEN livers. Magnetic resonance imaging revealed increased numbers of intrahepatic cysts and liver histology confirmed the presence of early HCC in alcohol-DEN mice compared to al l other groups. This correlated with increased serum alphafetoprotein, a marker of HCC, in alcohol-DEN mice. PCNA immunostaining revealed significantly increased hepatocyte proliferation in livers from alcohol-DEN compared to pair fed-DEN or alcohol-fed mice.CONCLUSION: We describe a new 12-wk HCC model in adult mice that develops in livers with alcoholic hepatitis and defines ALD as co-factor in HCC. | Aditya Ambade Abhishek Satishchandran Benedek Gyongyosi Patrick Lowe Gyongyi Szabo | 2016 | World Journal of Gastroenterology2016，22，16： | 4 |
| 10 | Korean red ginseng promotes hippocampal neurogenesis in mice显示文摘Neurogenesis in the adult hippocampus plays a major role in cognitive ability of animals including learning and memory.Korean red ginseng (KRG) has long been known as a medicinal herb with the potential to improve learning and memory;however,the mechanisms are still elusive.Therefore,we evaluated whether KRG can promote cognitive function and enhance neurogenesis in the hippocampus.Eight-week-old male C57BL/6 mice received 50 mg/kg of 5-bromo-2′-deoxyuridine (BrdU) intraperitoneally and 100 mg/kg of KRG or vehicle orally once a day for 14 days.Pole,Rotarod and Morris water maze tests were performed and the brains were collected after the last behavioral test.Changes in the numbers of BrdU- and BrdU/ doublecortin (DCX;a marker for neuronal precursor cells and immature neurons)-positive cells in the dentate gyrus and the gene expression of proliferating cell nuclear antigen (a marker for cell differentiation),cerebral dopamine neurotrophic factor and ciliary neurotrophic factor in the hippocampus were then investigated.KRG-treated mice came down the pole significantly faster and stood on the rotarod longer than vehicle-treated mice.The Morris water maze test showed that KRG administration enhanced the learning and memory abilities significantly.KRG also significantly increased BrdU- and BrdU/DCX-positive cells in the dentate gyrus as well as the proliferating cell nuclear antigen,cerebral dopamine neurotrophic factor and ciliary neurotrophic factor mRNA expression levels in the hippocampus compared to vehicle.Administration of KRG promotes learning and memory abilities,possibly by enhancing hippocampal neurogenesis.This study was approved by the Pusan National University Institutional Animal Care and Use Committee (approval No.PNU-2016-1071) on January 19,2016. | Sun Ryu Hyongjun Jeon Hee-Young Kim Sungtae Koo Seungtae Kim | 2020 | Neural Regeneration Research2020，15，5： | 4 |
| 11 | RELATIONSHIP BETWEEN PROLIFERATING CELL NUCLEARANTIGEN EXPRESSION AND ITS MALIGNANCY POTENTIAL IN COLORECTAL CARCINOMA显示文摘Objective: To study the relationship betweenproliferating cell nuclear antigen expression and itsmalignancy potential in colorectal carcinoma. Methods:Paraffin sections of 86 patients with advanced colorectalcarcinoma were assessed by immunohistochemical study,using a mouse monoclonal antibody (pc-10, DAKO -Co.USA) to check proliferating cell nuclear antigen (PCNA).TO compare PCNA with conventional clinicopathologicfactor including p53 overexpression, tissuecarcinoembnyonic antigen immunoreactivity patternand now cytometric DNA ploidy for assessing tumormalignancy potential. In addition, recurrence andsurvival of patients with advanced colorectal carcinomaafter curative resection were analyzed in accordancewith degree of PCNA expression. Results: PCNAlabeling index (PCNA-LI) increased significantly as thetumor stage advanced (p=0.0001). Strong correlationswere observed between PCNA-LI and variouspathologic parameters, including histologicdifferentiation (P=0.0027), lymphatic invasion(P=0.0001), vascular invasion (P=0.0001), lymph nodemetastasis (P=0.0001), and liver metastasis (P=0.0036).Mean PCNA-LI was also significantly higher in tumorwith DNA aneuploidy (P=0.0006) and negative (P=0.01).Linear relationships were demonstrated between PCNALI and clinical outcomes; Recurrence rate wassignificantly greater in the group with higher than themean PCNA-LI, who underwent curative resection(P<0.01), and three-year survival rates for curativecases with higher than the mean PCNA-LI weresignificantly poorer than those with lower than meanPCNA-LI (P<0.005). Conclusion: There werecorrelations between PCNA-LI and various pathologicparameters, PCNA-LI increased significantly as thetumor stage advanced in colorectal carcinoma, the ratesof recurrence and death got higher as PCNA-LIincreased after curative resection for colorectalcarcInoma. | 肖小炜 | 1999 | Chinese Journal of Cancer Research1999，11，2： | 4 |
| 12 | Magnifying chromoendoscopy combined with immunohistochemical staining for early diagnosis of gastric cancer显示文摘AIM:To assess the diagnostic value of using magnifying chromoendoscopy combined with immunohisto-chemical staining of proliferating cell nuclear antigen (PCNA)and p53 in the detection of gastric precancerous lesions. METHODS:Ninety-five patients who were treated for abdominal discomfort,abdominal pain,bloating,and acid reflux at our hospital from January 2010 to December 2011 were included in the study.An ordinary gastroscopic procedure was initially performed to select the lesions.All subjects underwent magnifying chromo-endoscopy to observe morphological changes of gastric pits.Biopsies were then taken from each area of interest and sent for pathological examination and detection of PCNA and p53 expression by immunohistochemistry. An immunoreactivity score for each lesion was calcu-lated.Based on immunoreactivity scores,immunohisto-chemical staining was then considered. RESULTS:Compared to intestinal metaplasia,gastric pits were more diverse in size,more irregular in shape, and more disorderly in arrangement in moderate and severe dysplasia.PCNA and p53 expression was sig-nificantly higher in precancerous lesions(intestinal metaplasia and dysplasia)than in chronic gastritis. PCNA expression showed an upward trend in types A-F pits.The number of cases that showed strong PCNA positivity increased significantly with an increase in the severity of lesions.Rank sum test for independent samples showed that p53 expression was significantly higher in types E and F pits than in types A-D pits(H =33.068,P=0.000).Rank sum test for independent samples showed that PCNA expression was significantly higher in types E and F pits than in types A-D pits(H =31.791,P=0.001). CONCLUSION:The presence of types E and F pits,in which p53 and PCNA are highly expressed,is highly sug- gestive of the occurrence of early cancer,and patients developing these changes should be closely followed. | Xian-Mei Meng Yi Zhou Tong Dang Xu-Yang Tian Jie Kong | 2013 | World Journal of Gastroenterology2013，19，3： | 2 |
| 13 | Role of Helicobacter Pylori Infection in Pathogenesis of Gastric Adenocarcinoma显示文摘GastriccancerisbelievedtobeoneofthemostcommonmalignanciesamongChi-nesepopulation.Inrecentyears,studiesonriskfactorsofgastricc... | 任宏宇 易粹琼 | 1999 | Journal of Huazhong University of Science and Technology(Medical Sciences)1999，19，2： | 2 |
| 14 | Maintaining proton homeostasis is an essential role of the Warburg effect in proliferating cells显示文摘Non-proliferating cells efficiently generate adenosine 5’-triphosphate (ATP) through mitochondrial oxidative phosphorylation.By contrast, proliferating cells, including cancer cells, tend to rely on aerobic glycolysis, an inefficient way to generate energy, and this phenomenon is termed 'the Warburg effect'1,2.However, the advantage of the Warburg effect provided for proliferating cells has been unclear3.Here we propose that aerobic glycolysis may maintain proton homeostasis to benefit proliferating cells. | Chuangzhen Yang Binghui Li | 2019 | Cancer Biology & Medicine2019，16，3： | 2 |
| 15 | Effect of EGF on initiation of primordial follicle growth in ovary of newborn rat显示文摘The present study was designed to look at the effect of epidermal growth factor (EGF) and tomcie-stimulating hormone (FSH) on initiation of primordial follicle growth and differentiation in the ovary of newborn rat with a sensitive marker of proliferating cell nuclear antigen (PCNA). The results showed that more cuboidal granulosa cells (GC) were found in the ovary two days after injection of EGF. More proliferative GC were observed on D4. No such action of FSH on primordial follicles was demonstrated. Using in situ hybridization, inhibin a mRNA expression in GC was detected from D5, while FSH receptor (FSHR) mRNA expression started from D6 after birth. Both mRNAs increased following further development of the follicles. These results suggest that it is EGF, but not FSH, that may play a certain role in initiation of primordial follicle growth. FSH may be involved in further differentiation and growth of the early developmental follicles. | 柳海珍 许复华 刘以训 | 2000 | Science China(Life Sciences)2000，43，5： | 1 |
| 16 | The pathological changes of inflammatory cells,smooth muscle cell and neo-vessels in the vulnerable carotid atherosclerosis plaque显示文摘Objective To study the inflammation, smooth muscle cells and neovessels change in the vulnerable carotid atherosclerosis plaque. Methods 6 male patients, aged between 66~73 years old, had the history of stroke or transient cerebral ischemic attacks of internal carotid artery system in a few days to 5 months. MRI and DSA re- | 吕鹤 | 2006 | China Medical Abstracts(Internal Medicine)2006，23，4： | 1 |
| 17 | EXPERIMENTAL RESEARCH ON THE ANTI-CANCER IMMUNOMODULA-TIVE EFFECT OF THE POLYSOCOHARIBE-PEPTIDE OF CORIOLUS VERSICOLOR显示文摘Polysocoharibe-peptide of Coriolus Versicolor (PSP) is a new anti-cancer immunomodulative drug. The present paper reports on the experimental research done with this drug. It was found that PSP had the ability to recover hemolysin HC50, to increase the weight of the thymus, and increase the alexin of serum C3 and the IgG content of tumor bearing mice. FSP also significantly raised the pha-gocytic activity of macrophages in normal mice. PSP had a significant inhibitory effect on P38S and S180 cells. At the concentration of 1 mg/ml, PSP inhibited the proliferating activity of some human tumor call lines, such as SGC 7901, SPC, SLY and Mei. It had a direct toxic effect on SPC cells. PSP significantly inhibited the synthesis of nucleic acids of Ehrlich ascites carcinoma cells. In addition, PSP was antagonistic to the side effects of chemotherapy and radiotherapy. | 许良中 杨庆尧 周金煦 陈瑞婷 李晓玉 胡玉娟 王筠默 | 1989 | Chinese Journal of Cancer Research1989，1，2： | 1 |
| 18 | Expression of p27kip1, Rb protein and proliferating cell nuclear antigen and its relationship with clinicopathology in human pancreatic cancer显示文摘OBJECTIVE: To investigate the effect of inhibiting factor of cell cycle regulation p27kipl,retinoblastinoma protein (Rb protein), and proliferating cell nuclear antigen (PCNA) on the genesis andprogression of human pancreatic cancer.METHODS: The expression of p27kipl, Rb protein and PCNA in the tumor tissue and adjacent tissue of32 patients with pancreatic cancer was detected by SP immunohistochemical technique.RESULTS: The p27kipl protein positive-expression rate in the tumor tissue of pancreatic cancer was56.25%, which was lower than that in the adjacent pancreatic tissue (P<0.05). p27kipl proteinpositive-expression was correlated significantly with tumor cell differentiation and lymph node metastasis(P<0.05). The Rb gene protein positive-expression rate in the tumor tissue was 50%, which was alsolower than that in the adjacent pancreatic tissue (P<0.05 ). The PCNA positive-expression rate was71.87%, which was higher than that in the adjacent pancreatic tissue (P<0.05). PCNA positive-expression was also correlated significantly with tumor cell differentiation and lymph node metastasis(P<0.05).CONCLUSION: The decreased expression of p27kipl, Rb protein and over-expression of PCNA may playan important role in the genesis and progression of pancreatic cancer. | | 2003 | Hepatobiliary & Pancreatic Diseases International2003，2，1： | 1 |
| 19 | Reliable experimental model of hepatic veno-occlusive disease caused by monocrotaline显示文摘BACKGROUND:Hepatic veno-occlusive disease(HVOD)is a severe complication of chemotherapy before hematopoietic stem cell transplantation and dietary ingestion of pyrrolizidine alkaloids.Many experimental models were established to study its mechanisms or therapy,but few are ideal.This work aimed at evaluating a rat model of HVOD induced by monocrotaline to help advance research into this disease. METHODS:Thirty-two male rats were randomly classified into 5 groups,and PBS or monocrotaline was administered (100 mg/kg or 160 mg/kg).They were sacrificed on day 7(groups A,B and D)or day 10(groups C and E).Blood samples were collected to determine liver enzyme concentrations.The weight of the liver and body and the amount of ascites were measured.Histopathological changes of liver tissue on light microscopy were assessed by a modified Deleve scoring system.The positivity of proliferating cell nuclear antigen(PCNA)was estimated. RESULTS:The rats that were treated with 160 mg/kg monocrotaline presented with severe clinical symptoms (including two deaths)and the histopathological picture of HVOD.On the other hand,the rats that were fed with 100 mg/kg monocrotaline had milder and reversible manifestations.Comparison of the rats sacrificed on day 10 with those sacrificed on day 7 showed that the positivity of PCNA increased,especially that of hepatocytes.CONCLUSIONS:Monocrotaline induces acute,dose- dependent HVOD in rats.The model is potentially reversible with a low dose,but reliable and irreversible with a higher dose.The modified scoring system seems to be more accurate than the traditional one in reflecting the histopathology of HVOD.The enhancement of PCNA positivity may be associated with hepatic tissue undergoing recovery. | | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，4： | 1 |
| 20 | The Effectof the Lyso PC-induced Endothelial Cell Conditioned Medi-um on Proliferating Cell Nuclear Antigen Expression of the Calf Tho-racic Aorta Smooth Muscle Cell显示文摘In order to study the effect of and mechanism of lysophosphatidylcholine (LysoPC) on proliferation of the calf thoracic aorta smooth muscle cells (ASMCs), the ASMCs were used to observe the effects of LysoPC induced endothelial cell conditioned medium on the DNA content and proliferating cell nuclear antigen (PCNA) expression in the calf thoracic ASMCs by flow cytometry and Western Blot technique. It was found that LysoPC induced endothelial cell conditioned medium could significantly promote PCNA expression of the calf ASMCs, induce the converting of ASMCs from G 0 /G 1 phase to S phase of DNA synthesis, and increase the tyrosine phosphorylation protein expression. Tyrosine protein kinase inhibitor (TPKi) RG50864 could obviously inhibit proliferation of LysoPC induced ASMCs in a dose dependence manner. The results indicated that the effect of LysoPC promoting the proliferation of ASMCs is partly evoked by endothelial cell derived growth factors such as PDGF and so on. | 周洪莲 姚济华 余枢 | 2002 | Journal of Huazhong University of Science and Technology(Medical Sciences)2002，22，1： | 0 |
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