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| 1 | Loss of BRCA1 expression leads to worse survival in patients with gastric carcinoma显示文摘AIM: To investigate the expression deficiency of key molecular markers in the homologous recombination pathway. METHODS: Expression loss of breast cancer type 1 susceptibility protein (BRCA1), ataxia telangiectasia mutated (ATM), ATM-Rad3-related (ATR), mediator of DNA damage checkpoint protein 1 (MDC1) and meiotic recombination 11 (Mre11) were correlated with their clinicopathological parameters in gastric cancer (GC). One hundred and twenty treatment-naive GC samples were formalin-fixed and paraffin-embedded into tissue blocks. Two representative cores from each block were extracted and constructed into tissue microarrays. Expression levels of BRCA1, ATM, ATR, MDC1 and Mre11 were determined using immunohistochemical analysis, and correlated with clinical parameters, including age, gender, Lauren subtype, tumor grades, clinical stage and overall survival.RESULTS: Expression loss of BRCA1, ATM, ATR, MDC1, and Mre11 was found in 21.4%, 20.2%, 21.0%, 11.1% and 4.6%, respectively, of interpretable cases. BRCA1 loss was significantly associated with patients of diffused subtype (intestinal vs diffused, 8.2% vs 31.7%, P = 0.001), higher tumor grade (Ⅰ/Ⅱ vs Ⅲ, 10.7% vs 20.5;Ⅰ/Ⅱ vs Ⅳ, 10.7% vs 54.5%, P = 0.047) and advanced clinical stage (Ⅰ/Ⅱ vs Ⅲ, 12.9% vs 16.9%;Ⅰ /Ⅱ vs Ⅳ, 12.9% vs 45.5%, P = 0.006). MDC1 loss was significantly associated with patients of diffused subtype (intestinal vs diffused, 0% vs 19.7%, P = 0.001) and higher tumor grade (Ⅰ/Ⅱ vs Ⅲ, 0% vs 12%;Ⅰ/Ⅱ vs Ⅳ, 0% vs 30.8%, P = 0.012). In addition, the survival time of the patients with expression loss of BRCA1 was significantly shorter than those with positive expression of BRCA1 (2-year survival rate, 32.4% vs 62.8%, P = 0.015). No correlations were found between clinicopathological parameters and expression loss of ATM, ATR and Mre11. CONCLUSION: Our results support the hypothesis that homologous recombination deficiency plays an important role in the progression of gastric carcinoma. Loss of expression of BRCA1 and MDC1 may serve as predictive factors in tumor development or progression in GC patients. | Zi-Zhen Zhang Yuan Jie Charles Liu Xiao-Lu Yin Ping Zhan Yi Gu Xing-Zhi Ni | 2013 | World Journal of Gastroenterology2013，19，12： | 11 |
| 2 | Molecular Epidemiology of Echovirus 18 Circulating in China's Mainland from 2015 to 2016显示文摘Echovirus 18(E18), a serotype of Enterovirus B(EV-B) species, is an important pathogen in aseptic meningitis. E18 had rarely been detected in China's Mainland, but became the predominant pathogen associated with viral encephalitis(VE) and meningitis in Hebei province for the first time in 2015. To investigate the molecular epidemiology and genetic characteristics of E18 in China's Mainland, sixteen E18 strains from patient throat swabs with hand, foot, and mouth disease(HFMD) in six provinces in China collected between 2015 and 2016, and four E18 strains isolated from 18 patient cerebrospinal fluid specimens with VE in Hebei Province in 2015 were obtained and sequenced. Combined with the sequences from the GenBank database, we performed an extensive genetic analysis. Phylogenetic analysis of VP1 gene sequences revealed that all E18 strains from China's Mainland after 2015 belonged to subgenotype C2. There were no obvious specific differences in phylogenetic and variation analyses of E18 genome sequences between HFMD and VE/meningitis strains. Potential multiple recombination may have occurred in the 50-untranslated region and in the P2 and P3 nonstructural protein-encoding regions of E18 strains from China. The current E18 strains were potential multiplerecombinant viruses. Overall, these findings supported that E18 caused HFMD, VE, and meningitis, although there were no significant associations between clinical features and viral genomic characteristics. | Xiangpeng Chen Tianjiao Ji Jiayun Guo Wei Wang Wenbo Xu Zhengde Xie | 2019 | Virologica Sinica2019，34，1： | 10 |
| 3 | APOBEC deaminases-mutases with defensive roles for immunity显示文摘In recent years, tremendous progress has been made in the elucidation of the biological roles and molecular mechanisms of the apolioprotein B mRNA-editing enzyme catalytic polypeptide (APOBEC) family of enzymes. The APOBEC family of cytidine deaminases has important functional roles within the adaptive and innate immune system. Activation induced cytidine deaminase (AID) plays a central role in the biochemical steps of somatic hypermutation and class switch recombination during antibody maturation, and the APOBEC 3 enzymes are able to inhibit the mobility of retroelements and the replication of retroviruses and DNA viruses, such as the human immunodeficiency virus type-1 and hepatitis B virus. Recent advances in structural and functional studies of the APOBEC enzymes provide new biochemical insights for how these enzymes carry out their biological roles. In this review, we provide an overview of these recent advances in the APOBEC field with a special emphasis on AID and APOBEC3G. | Courtney PROCHNOW Ronda BRANSTEITTER | 2009 | Science China(Life Sciences)2009，52，10： | 6 |
| 4 | Linkage analysis and integrated software GAPL for pure-line populations derived from four-way and eight-way crosses显示文摘Pure lines derived from multiple parents provide abundant variation for genetic study.However,efficient genetic analysis methods and user-friendly software are still lacking.In this study, we developed linkage analysis methods and integrated analysis software for pure-line populations derived from four-way and eight-way crosses.First, polymorphic markers are classified into different categories according to the number of identifiable alleles in the inbred parents.Expected genotypic probability is then derived for each pair of complete markers, and based on them a maximum likelihood estimate(MLE) of recombination frequency is calculated.An EM algorithm is proposed for calculating recombination frequencies in scenarios that at least one marker is incomplete.A linkage map can thus be constructed using estimated recombination frequencies.We describe a software package called GAPL for recombination frequency estimation and linkage map construction in multi-parental pure-line populations.Both simulation studies and results from a reported four-way cross recombinant inbred line population demonstrate that the proposed method and software can build more accurate linkage maps in shorter times than other published software packages.The GAPL software is freely available from www.isbreeding.net and can also be used for QTL mapping in multi-parental populations. | Luyan Zhang Lei Meng Jiankang Wang | 2019 | The Crop Journal2019，7，3： | 5 |
| 5 | Co-evolution of plant LTR-retrotransposons and their host genomes显示文摘Transposable elements(TEs),particularly,long terminal repeat retrotransposons(LTR-RTs),are the most abun-dant DNA components in all plant species that have been investigated,and are largely responsible for plant genome size variation.Although plant genomes have experi-enced periodic proliferation and/or recent burst of LTR-retrotransposons,the majority of LTR-RTs are inactivated by DNA methylation and small RNA-mediated silencing mechanisms,and/or were deleted/truncated by unequal homologous recombination and illegitimate recombina-tion,as suppression mechanisms that counteract genome expansion caused by LTR-RT amplifi cation.LTR-RT DNA is generally enriched in pericentromeric regions of the host genomes,which appears to be the outcomes of pref-erential insertions of LTR-RTs in these regions and low effectiveness of selection that purges LTR-RT DNA from these regions relative to chromosomal arms.Potential functions of various TEs in their host genomes remain blurry;nevertheless,LTR-RTs have been recognized to play important roles in maintaining chromatin structures and centromere functions and regulation of gene expres-sions in their host genomes. | Meixia Zhao Jianxin Ma | 2013 | Protein & Cell2013，4，7： | 4 |
| 6 | Role of ubiquitination in meiotic recombination repair显示文摘Programmed and unprogrammed double-strand breaks (DSBs) often arise from such physiological requirements as meiotic recombination, and exogenous insults, such as ionizing radiation (IR). Due to deleterious impacts on genome stability, DSBs must be appropriately processed and repaired in a regulatory manner. Recent investigations have indicated that ubiquitination is a critical factor in DNA damage response and meiotic recombination repair. This review summarizes the effects of proteins and complexes associated with ubiquitination with regard to homologous recombination (HR)-dependent DSB repair. | CUI Li & LI Wei State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China | 2010 | Science China(Life Sciences)2010，53，4： | 4 |
| 7 | Biexciton Auger recombination in mono-dispersed, quantum-confined CsPbBr3 perovskite nanocrystals obeys universal volume-scaling显示文摘Auger recombination has been a long?standing obstacle to many prospective applications of colloidal quantum dots (QDs) ranging from lasing, light-emitting diodes to bio-labeling. As such, understanding the physical underpinnings and scaling laws for Auger recombination is essential to these applications. Previous studies of biexciton Auger recombination in various QDs established a universal scaling of biexciton lifetime (rxx) with QD volume (V):τxx =γV. However, recent measurements on perovskite nanocrystals (NCs), an emerging class of enablers for light harvest!ng and emitting applications, showed significant deviations from this universal scaling law, likely because the measured NCs are weakly-confined and also have relatively broad size-distributions. Here we study biexciton Auger recombination in mono-dispersed (size distributions within 1.7%—9.0%), quantunvconfined CsPbBr3 NCs (with confinement energy up to 410 meV) synthesized using a latest approach based on thermodynamic equilibrium control. Our measurements clearly reproduce the volume-scaling of τxx in confined CsPbBb QDs. However, the scaling factor γ(0.085 ± 0.001 ps/nm^3) is one order of magnitude lower than that reported for CdSe and PbSe QDs (1.00 ± 0.05 ps/nm^3), suggest!ng unique mechanisms enhancing Auger recombination rate in perovskite NCs. | Yulu Li Tao Ding Xiao Luo Zongwei Chen Xue Liu Xin Lu Kaifeng Wu | 2019 | Nano Research2019，12，3： | 4 |
| 8 | Recent progress in interface modification for dye-sensitized solar cells显示文摘Interface modification on the TiO2/dye/electrolyte interface of dye-sensitized solar cells (DSCs) is one of the most effective approaches to suppress the charge recombination,improve electron injection and transportation,and thus ameliorate the conversion efficiency and stability of DSCs.Conventional research focusing on the photoanodes interface modification before sensitization in dye-sensitized solar cells has been carried out and reviewed.However,recent studies showed that post-modification after sensitization of the TiO2 electrode also plays a significant role on the TiO2/dye/electrolyte interface.This post-modification using the immersing method could deprotonate dye molecules,prohibit the dye aggregation and retard the recombination reaction.As a result,it has great influence on the devices' photovoltaic performance.This interface modification could also provide an approach to broaden the response of the solar spectrum by introducing an alternative assembling structure.An in-situ meaning of using a co-adsorbent is employed to modify the interface in the DSCs,which could retard the aggregation of the dye molecules and enhance the conversion efficiency.In addition,electrolyte additives can be used to modify the TiO2/dye/electrolyte interface through some unique mechanisms.Based on the background of interface modification of photoanodes before sensitization,this review introduces various interface modifications after sensitization of dye-sensitized solar cells and their mechanisms. | MA BeiBei,GAO Rui,WANG LiDuo,ZHU YiFeng,SHI YanTao,GENG Yi,DONG HaoPeng & QIU Yong Key Lab of Organic Optoelectronics & Molecular Engineering of Ministry of Education Department of Chemistry,Tsinghua University,Beijing 100084,China | 2010 | Science China Chemistry2010，53，8： | 4 |
| 9 | Ikaros in B cell development and function显示文摘The zinc finger transcription factor,Ikaros,is a central regulator of hematopoiesis.It is required for the development of the earliest B cell progenitors and at later stages for VDJ recombination and B cell receptor expression.Mature B cells rely on Ikaros to set the activation threshold for various stimuli,and to choose the correct antibody isotype during class switch recombination.Thus,Ikaros contributes to nearly every level of B cell differentiation and function. | MacLean Sellars Philippe Kastner Susan Chan | 2011 | World Journal of Biological Chemistry2011，2，6： | 3 |
| 10 | The KLL dielectronic recombination processes for highly charged krypton,iodine and barium ions显示文摘The KLL dielectronic recombination (DR) processes of ions from highly charged helium-like to oxygen-like krypton,iodine and barium ions are studied systematically in the relativistic distorted-wave approximation with configuration interaction.The KLL DR resonant energies,the corresponding resonant strengths and the theoretical spectra for each highly charged ion species are obtained.The results accord well with other available values.The behaviour of KLL resonant strengths for He-like ions with atomic number Z is analysed. | 杨建会 张红 程新路 | 2010 | Chinese Physics B2010，19，6： | 3 |
| 11 | Tracking SARS-CoV-2 Omicron diverse spike gene mutations identifies multiple inter-variant recombination events显示文摘The current pandemic of COVID-19 is fueled by more infectious emergent Omicron variants.Ongoing concerns of emergent variants include possible recombinants,as genome recombination is an important evolutionary mechanism for the emergence and re-emergence of human viral pathogens.In this study,we identified diverse recombination events between two Omicron major subvariants(BA.1 and BA.2)and other variants of concern(VOCs)and variants of interest(VOIs),suggesting that co-infection and subsequent genome recombination play important roles in the ongoing evolution of SARS-CoV-2.Through scanning high-quality completed Omicron spike gene sequences. | Junxian Ou Wendong Lan Xiaowei Wu Tie Zhao Biyan Duan Peipei Yang Yi Ren Lulu Quan Wei Zhao Donald Seto James Chodosh Zhen Luo Jianguo Wu Qiwei Zhang | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 3 |
| 12 | S-,N-and C-doped ZnO as semiconductor photocatalysts:A review显示文摘In the past few decades,many novel non-metal doped ZnO materials have developed hasty interest due to their adaptable properties such as low recombination rate and high activity under the solar light exposure.In this article,we compiled recent research advances in non-metal(S,N,C)doped ZnO,emphasizing on the related mechanism of catalysis and the effect of non-metals on structural,morphological,optical and photocatalytic characteristics of ZnO.This review will enhance the knowledge about the advancement in ZnO and will help in synthesizing new ZnO-based materials with modified structural and photocatalytic properties. | Vijaya KUMARI Anuj MITTAL Jitender JINDAL Suprabha YADAV Naveen KUMAR | 2019 | Frontiers of Materials Science2019，13，1： | 3 |
| 13 | Inhibition of KU70 and KU80 by CRISPR interference,not NgAgo interference,increases the efficiency of homologous recombination in pig fetal fibroblasts显示文摘Non-homologous end-joining(NHEJ) is a predominant pathway for the repair of DNA double-strand breaks(DSB). It inhibits the efficiency of homologous recombination(HR) by competing for DSB targets. To improve the efficiency of HR, multiple CRISPR interference(CRISPRi) and Natronobacterium gregoryi Argonaute(NgAgo) interference(NgAgoi) systems have been designed for the knockdown of NHEJ key molecules, KU70, KU80, polynucleotide kinase/phosphatase(PNKP), DNA ligase IV(LIG4), and NHEJ1. Suppression of KU70 and KU80 by CRISPRi dramatically promoted(P<0.05) the efficiency of HR to 1.85-and 1.58-fold, respectively, whereas knockdown of PNKP, LIG4, and NHEJ1 repair factors did not significantly increase(P>0.05) HR efficiency. Interestingly, although the NgAgoi system significantly suppressed(P<0.05) KU70, KU80, PNKP, LIG4, and NHEJ1 expression, it did not improve(P>0.05) HR efficiency in primary fetal fibroblasts. Our result showed that both NgAgo and catalytically inactive Cas9(dCas9) could interfere with the expression of target genes, but the downstream factors appear to be more active following CRISPR-mediated interference than that of NgAgo. | LI Guo-ling QUAN Rong WANG Hao-qiang RUAN Xiao-fang MO Jian-xin ZHONG Cui-li YANG Huaqiang LI Zi-cong GU Ting LIU De-wu WU Zhen-fang CAI Geng-yuan ZHANG Xian-wei | 2019 | Journal of Integrative Agriculture2019，18，2： | 2 |
| 14 | Pathways and assays for DNA double-strand break repair by homologous recombination显示文摘Double strand breaks(DSBs)are the most detrimental type of DNA damage that must be repaired to ensure genome integrity and cell survival.Unrepaired or improperly repaired DSBs can potentially cause tumorigenesis or cell death.DSBs are primarily repaired by non-homologous end joining or homologous recombination(HR).The HR pathway is initiated by processing of the 5′-end of DSBs to generate 3′-end single-strand DNA(ssDNA).Furthermore,the intermediate is channeled to one of the HR sub-pathways,including:(i)double Holliday junction(dHJ)pathway,(ii)synthesis-dependent strand annealing(SDSA),(iii)break-induced replication(BIR),and(iv)single-strand annealing(SSA).In the dHJ sub-pathway,the 3′-ssDNA coated with Rad51 recombinase performs homology search and strand invasion,forming a displacement loop(D-loop).Capture of the second end by the D-loop generates a dHJ intermediate that is subsequently dissolved by DNA helicase or resolved by nucleases,producing non-crossover or crossover products.In SDSA,the newly synthesized strand is displaced from the D-loop and anneals to the end on the other side of the DSBs,producing non-crossovers.In contrast,BIR repairs one-end DSBs by copying the sequence up to the end of the template chromosome,resulting in translocation or loss of heterozygosity.SSA takes place when resection reveals flanking homologous repeats that can anneal,leading to deletion of the intervening sequences.A variety of reporter assays have been developed to monitor distinct HR sub-pathways in both Saccharomyces cerevisiae and mammals.Here,we summarize the principles and representative assays for different HR sub-pathways with an emphasis on the studies in the budding yeast. | Jinbao Li Huize Sun Yulin Huang Yali Wang Yuyan Liu Xuefeng Chen | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 2 |
| 15 | Evolutionary rates of mitochondrial sequences and gene orders in Spirurina(Nematoda)are episodic but synchronised显示文摘In contrast to highly conserved mitogenomic architecture in most metazoan lineages,which indicates that rearrangement events are generally strongly selected against,a limited number of often unrelated lineages exhibit highly elevated architectural evolution rates.The underlying reasons for this discontinuity in the mitogenomic evolution remain unknown.Previously we sequenced the mitochondrial genome of the first Camallanoidea species,Camallanus cotti(Nematoda:Chromadorea:Spirurina:Camallanidae),and found that it exhibited a highly disrupted architecture.We hypothesised that disrupted architecture might be a synapomorphic feature of the sister-clades Camallanoidea and Dracunculoidea.In this study,we sequenced mitogenomes of three freshwater fish-parasitic nematodes:Camallanus lacustris(Camallanidae),and two Philometridae(Dracunculoidea)species,Clavinema parasiluri,and Philometra sp.In partial agreement with the working hypothesis,both Camallanoidea species had exceptionally large mitogenomes of 18–19 Kbp,albeit the underlying reasons differed:in C.lacustris it was the existence of a single enlarged noncoding region of5.5 Kbp.A segment of this region exhibited an inverted base composition skew,which is indicative of a sequence inversion or recombination event.Camallanidae is the second identified chromadorean(first for Spirurina)family that exhibits within-family protein-coding gene rearrangements,and the absence of trnL1 and trnF may be a synapomorphy for Camallanoidea.The underlying reason for the disrupted architecture of Camallanidae does not appear to be a particular event shared by their common ancestor,but rather an underlying mechanism that makes disruptive events more likely in this lineage.In disagreement with the working hypothesis,Spiruromorpha and Oxyuridomorpha exhibited even more highly rearranged gene orders and greater overall branch lengths than Camallanomorpha.However,withininfraorder architecture was highly conserved and leaf nodes very short.This indicates that common ancestors of Spiruromorpha and Oxyuridomorpha clades underwent a period of rapid mitochondrial evolution(both sequence and architecture),followed by a stabilisation after the taxonomic radiation.In contrast to this,Camallanomorpha,and particularly Camallanidae,appear to have entered a period of elevated evolutionary rates after the initial radiations of these two taxa.As a result of this evolutionary discontinuity,there was a strong correlation between the gene order rearrangement rate(GORR)and the overall branch length(0.81),but there was no correlation between the strength of purifying selection(ω?dN/dS)and the overall branch lengths(0.05)and GORR(0.04).These findings have important repercussions for future phylogenetic and other evolutionary studies of Spirurina. | Hong Zou Hong-Peng Lei Rong Chen Fang-Lin Chen Wen-Xiang Li Ming Li Dong Zhang Ivan Jakovlic Gui-Tang Wang | 2022 | Water Biology and Security2022，1，2： | 2 |
| 16 | A novel three primers PCR (TP-PCR) method to obtain recombinant DNA molecule independent of restriction enzyme显示文摘In this note, we report a novel and efficient three primers PCR (TP-PCR) method to rapidly generate recombinant DNA molecule at precise junction between two arbitrary DNA fragments. TP-PCR method is characterized by its reaction system with two templates and three primers, which can produce a recombinant DNA molecule in one PCR reaction. The main advantages of this method are the independence of sequences at the recombination site, the rapid-ness, and the easy establishment of adequate conditions. This method has been successfully applied to constructing a fusion protein gene, sck gene. | Chaoyang Deng Guisheng Song Junwang Xu Zhen Zhu | 2002 | Chinese Science Bulletin2002，47，24： | 2 |
| 17 | Partial strands synthesizing leads to inevitable aborting and complicated products in consecutive polymerase chain reactions (PCRs)显示文摘Various abnormal phenomena have been observed during PCR so far. The present study performed a series of consecutive PCRs (including many rounds of re-amplification continuously) and found that the abortion of re-amplification was inevitable as long as a variety of complicated product appeared. The aborting stages varied, according to the lengths of targets. Longer targets reached the abortion earlier than the shorter ones, marked by appearance of the complex that was immobile in electropho-resis. Denatured gel-electrophoresis revealed that the complex was mainly made up of shorter or partially synthesized strands, together with small amounts of full-length ones. Able to be digested by S1 nuclease but unable by restriction endonucleases (REs), the complex was proved to consist of both single regions and double-helix regions that kept the complex stable thermodynamically. Simulations gave evidence that partial strands, even at lower concentration, could disturb re-amplification effec- tively and lead to the abortion of re-amplifications finally. It was pointed out that the partial strands formed chiefly via polymerase’s infidelity, and hence the solution to lighten the abnormality was also proposed. | LUO Rui1,2 & ZHANG DaMing 1 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2 Graduate University of Chinese Academy of Sciences,Beijing 100049, China | 2007 | Science China(Life Sciences)2007，50，4： | 2 |
| 18 | Preface to the special topic on genome editing research in China显示文摘Genome engineering technologies enable the precise modification,regulation and tagging of genomic loci in living cells and organisms;leading to a broad range of applications from basic biology to biotechnology and biomedicine.For many years,precise genome manipulation has heavily relied on the homologous recombination(HR)-based gene targeting strategy,which has been limited to several organisms(yeast,mouse and human).However,recent revolutionary findings on bacterial RNA-guided clustered regularly interspaced short palindromic repeat(CRISPR)-Cas systems have led to a range of simple,fast and efficient genome engineering technologies,which are applicable to a wide range of organisms,including species that are recalcitrant to genetic manipulation via the traditional HR-based method. | Jinsong Li Caixia Gao | 2019 | National Science Review2019，6，3： | 2 |
| 19 | Defect passivation through electrostatic interaction for high performance flexible perovskite solar cells显示文摘The light weight,good bending resistance and low production cost make flexible perovskite solar cells(PSCs)good candidates in wearable electronics,portable charger,remote power,and flying objects.High power conversion efficiency(PCE)plays a crucial role on obtaining the high mass specific power of flexible devices.However,the performance for flexible PSCs is still having a large room to be improved.Here,we added the 2-amino-5-cyanopyridine(ACP)molecule with a polar electron density distribution in the perovskite precursor solution to improve the performance of flexible PSCs.The cyano groups with electron-withdrawing ability are expected to passivate positively charged point defects,while amines with electron donating ability are expected to passivate negatively charged point defects in perovskite films.Thanks to the effective passivation of defects at the grain boundary and surface of perovskite films,the PCE of flexible PSCs is obviously increased from 16.9%to 18.0%.These results provide a universal approach to improve performance of flexible PSCs by healing the defects in perovskite films through electrostatic interactions. | Deyu Xin Shujie Tie Xiaojia Zheng Jianguo Zhu Wen-Hua Zhang | 2020 | Journal of Energy Chemistry2020，29，7： | 2 |
| 20 | The high diversity of SARS-CoV-2-related coronaviruses in pangolins alerts potential ecological risks显示文摘Understanding the zoonotic origin and evolution history of SARS-CoV-2 will provide critical insights for alerting and preventing future outbreaks.A significant gap remains for the possible role of pangolins as a reservoir of SARS-CoV-2 related coronaviruses(SC2r-CoVs).Here,we screened SC2r-CoVs in 172 samples from 163 pangolin individuals of four species,and detected positive signals in muscles of four Manis javanica and,for the first time,one M.pentadactyla.Phylogeographic analysis of pangolin mitochondrial DNA traced their origins from Southeast Asia.Using in-solution hybridization capture sequencing,we assembled a partial pangolin SC2r-CoV(pangolin-CoV)genome sequence of 22895 bp(MP20)from the M.pentadactyla sample.Phylogenetic analyses revealed MP20 was very closely related to pangolin-CoVs that were identified in M.javanica seized by Guangxi Customs.A genetic contribution of bat coronavirus to pangolin-CoVs via recombination was indicated.Our analysis revealed that the genetic diversity of pangolin-CoVs is substantially higher than previously anticipated.Given the potential infectivity of pangolin-CoVs,the high genetic diversity of pangolin-CoVs alerts the ecological risk of zoonotic evolution and transmission of pathogenic SC2r-CoVs. | Min-Sheng Peng Jian-Bo Li Zheng-Fei Cai Hang Liu Xiaolu Tang Ruochen Ying Jia-Nan Zhang Jia-Jun Tao Ting-Ting Yin Tao Zhang Jing-Yang Hu Ru-Nian Wu Zhong-Yin Zhou Zhi-Gang Zhang Li Yu Yong-Gang Yao Zheng-Li Shi Xue-Mei Lu Jian Lu Ya-Ping Zhang | 2021 | Zoological Research2021，42，6： | 2 |
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