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| 1 | Effect of intestinal ischemia-reperfusion on expressions of endogenous basic fibroblast growth factor and transforming growth factor β in lung and its relation with lung repair显示文摘AIM To study the changes of endogenoustransforming growth factor β(TGFβ)and basicfibroblast growth factor(bFGF)in lung followingintestinal ischemia and reperfusion injury andtheir effects on lung injury and repair.METHODS Sixty Wistar rats were divided intofive groups,which underwent sham-operation,ischemia(45 minutes),and reperfusion(6,24and 48 hours,respectively)after ischemia(45minutes).Immunohistochemical method wasused to observe the localization and amounts ofboth growth factors.RESULTS Positive signals of both growthfactors could be found in normal lung,mainly inalveolar cells and endothelial cells of vein.Afterischemia and reperfusion insult,expressions ofboth growth factors were increased and theiramounts at 6 hours were larger than those ofnormal control or of 24 and 48 hours after insult.CONCLUSION The endogenous bFGF and TGF βexpression appears to be up-regulated in thelung following intestinal ischemia andreperfusion,suggesting that both growth factorsmay be involved in the process of lung injury andrepair. | Xiao Bing Fu Yin Hui Yang Tong Zhu Sun Xiao Man Gu Li Xian Jiang Xiao Oing Sun Zhi Yong Sheng Research Laboratory,304th Hospital of PLA,Beijing 100037,China | 2000 | World Journal of Gastroenterology2000，6，3： | 21 |
| 2 | bFGF and TGFβ expression in rat kidneys after ischemic/reperfusional gut injury and its relationship with tissue repair显示文摘INTRODUCTION Intestinal isehemia/rePerfusion(I/R)oeeureommonly in eritieally 111 Patients.It 15 wellreeognized that gutl/R may eause tissue damageand dysfunetion of intestine,and induee | Yang YH Fu XB Sun TZ Jiang LX Gu XM | 2000 | World Journal of Gastroenterology2000，6，1： | 13 |
| 3 | Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor显示文摘Objective:To study the effects of Prunella vulgaris polysaccharide(PVP)on human breast carcinoma-associated fibroblasts(CAFs).Method:Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)?2H?tetrazolium(MTS)assay.Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects.Flow cytometry was applied to detect cell apoptosis and cell cycle distribution.Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor(bFGF)in CAFs.Culture SKBr-3 with CAFs conditioned medium(CAFs-CM)to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells.Results:PVP inhibited the viability of CAFs by inducing apoptosis(P<0.01)and arresting cell cycle(P<0.01).It also inhibited the migration of CAFs(P<0.01).bFGF promoted CAFs proliferation(P<0.01)and migration(P<0.01),protected CAFs from apoptosis(P<0.05)and reduced Go phase to 49.06%(P<0.01).However,these effects of bFGF on CAFs could be abrogated by PVP.Culturing SKBr-3 with CAFs-CM,PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly.Moreover,PVP reduced the mRNA expression(P<0.01)and protein secretion of bFGF(P<0.01)in CAFs.Conclusion:PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expressi on,thus inhibit!ng the growth of breast can cer SKBr-3 cells in directly. | HAO Jian DING Xiu-li YANG Xue WU Xiong-zhi | 2020 | Chinese Journal of Integrative Medicine2020，26，4： | 12 |
| 4 | 外源性碱性成纤维细胞生长因子对创面愈合病理变化的影响显示文摘目的:探讨外源性碱性成纤维细胞生长因子(bFGF)在伤口愈合过程中可能的愈合机制以及对瘢痕形成的影响。方法:选用成年健康新西兰兔24只,建立兔耳创面愈合模型96个,随机分为bFGF治疗组和磷酸盐缓冲液(PBS)对照组,观察平均愈合时间、瘢痕体积、组织学变化及超微结构。结果:①bFGF组与对照组愈合时间分别为(18.4±1.6)d、(21.3±1.4)d,两组之间有显著性差异(P<0.05)。②bFGF组瘢痕体积明显小于对照组,两组间差异有统计学意义(P<0.01)。③光镜观察,bFGF组在愈合期炎症反应、血管形成及成纤维细胞增殖更明显。在愈合后的瘢痕组织中,胶原纤维排列较规则。对照组炎症反应时间较长,胶原纤维较粗大,排列紊乱。④电镜下可见治疗组成纤维细胞胶原合成持续时间较短。肥大细胞与成纤维细胞紧密接触,调整愈合全过程。后期表皮与真皮间有部分基底膜重建;对照组成纤维细胞合成持续时间较长,未见有基底膜的重建。结论:在伤口愈合过程中,外源性bFGF通过对细胞的调控有效地发挥促愈合作用;同时,通过部分重建基底膜结构,改善瘢痕的形成。 | 卞徽宁 陈华德 郑少逸 赖文 高辉 熊兵 刘族安 | 2006 | 感染.炎症.修复2006，7，4： | 9 |
| 5 | ^(125)I-labeled anti-b FGF monoclonal antibody inhibits growth of hepatocellular carcinoma显示文摘AIM: To investigate the inhibitory efficacy of ^(125)I-labeled anti-basic fibroblast growth factor(b FGF) monoclonal antibody(m Ab) in hepatocellular carcinoma(HCC).METHODS: b FGF m Ab was prepared by using the 1G9B9 hybridoma cell line with hybridization technology and extracted from ascites fluid through a Protein G Sepharose affinity column. After labeling with ^(125)I through the chloramine-T method, b FGF m Ab was further purified by a Sephadex G-25 column. Gamma radiation counter GC-1200 detected radioactivity of ^(125)I-b FGF m Ab. The murine H22 HCC xenograft model was established and randomized to interventions with control(phosphate-buffered saline), ^(125)I-b FGF m Ab,^(125)I plus b FGF m Ab, b FGF m Ab, or ^(125)I. The ratios of tumor inhibition were then calculated. Expression of b FGF, fibroblast growth factor receptor(FGFR), plateletderived growth factor, and vascular endothelial growth factor(VEGF) m RNA was determined by quantitative reverse transcriptase real-time polymerase chain reaction. RESULTS: The purified b FGF m Ab solution was 8.145 mg/m L with a titer of 1:2560000 and was stored at-20 ℃. After coupling, ^(125)I-b FGF m Ab was used at a 1: 1280000 dilution, stored at 4 ℃, and its specific radioactivity was 37 MBq/mg. The corresponding tumor weight in the control, ^(125)I, b FGF m Ab, ^(125)I plus b FGF m Ab, and ^(125)I-b FGF m Ab groups was 1.88 ± 0.25, 1.625 ± 0.21, 1.5 ± 0.18, 1.41 ± 0.16, and 0.98 ± 0.11 g, respectively. The tumor inhibition ratio in the ^(125)I, b FGF m Ab, ^(125)I plus b FGF m Ab, and ^(125)I-b FGF m Ab groups was 13.6%, 20.2%, 25.1%, and 47.9%, respectively. Growth of HCC xenografts was inhibited significantly more in the ^(125)I-b FGF m Ab group than in the other groups(P < 0.05). Expression of b FGF and FGFR m RNA in the ^(125)I-b FGF m Ab group was significantly decreased in comparison with other groups(P < 0.05). Groups under interventions revealed increased expression of VEGF m RNA(except for ^(125)I group) compared with the control group.CONCLUSION: ^(125)I-b FGF m Ab inhibits growth of HCC xenografts. The coupling effect of ^(125)I-b FGF m Ab is more effective than the concomitant use of ^(125)I and b FGF m Ab. | Peng-Hui Hu Lan-Hong Pan Patrick Ting-Yat Wong Wen-Hui Chen Yan-Qing Yang Hong Wang Jun-Jian Xiang Meng Xu | 2016 | World Journal of Gastroenterology2016，22，21： | 4 |
| 6 | Fibroblast Growth Factor-2 Counteracts the Effect of Ciliary Neurotrophic Factor on Spontaneous Differentiation in Adult Hippocampal Progenitor Cells显示文摘Neural stem/progenitor cells (NSCs) can spontaneously differentiate into neurons and glial cells in the absence of mitogen fibroblast growth factor-2 (FGF-2) or epidermal growth factor (EGF) in medium and the spontaneous differentiation of NSCs is mediated partially by endogenous ciliary neuro-trophic factor (CNTF). This study examined the relationship of FGF-2 and CNTF in the spontaneous differentiation of adult hippocampal progenitor cells (AHPs). AHPs were cultured in the medium containing different concentration of FGF-2 (1-100 ng/mL). Western blotting and immunofluorescence staining were applied to detect the expression of the astrocytic marker GFAP, the neuronal marker Tuj1, the oligodendrocytic marker CNPase and, Nestin, the marker of AHPs. The expression of endogenous CNTF in AHPs at early (passage 4) and late stage (passage 22) was also measured by Western blotting. The results showed that FGF-2 increased the expression of Nestin, dramatically inhibited the expression of GFAP and Tuj1 and slightly suppressed the expression of CNPase. FGF-2 down-regulated the expression of endogenous CNTF in AHPs at both early (passage 4) and late stage (passage 22). These results suggested that FGF-2 could inhibit the spontaneous differentiation of cultured AHPs by negatively regulating the expression of endogenous CNTF in AHPs. | 贺致礼 丁君 张建芳 刘颖 龚成新 孙圣刚 陈红辉 | 2012 | Journal of Huazhong University of Science and Technology(Medical Sciences)2012，32，6： | 3 |
| 7 | Multifunctional biomimetic spinal cord: New approach to repair spinal cord injuries显示文摘The incidence of spinal cord injury(SCI) has been gradually increasing, and the treatment has troubled the medical field all the time. Primary and secondary injuries ultimately lead to nerve impulse conduction block. Microglia and astrocytes excessively accumulate and proliferate to form the glial scar. At present, to reduce the effect of glial scar on nerve regeneration is a hot spot in the research on the treatment of SCI. According to the preliminary experiments, we would like to provide a new bionic spinal cord to reduce the negative effect of glial scar on nerve regeneration. In this hypothesis we designed a new scaffold that combine the common advantage of acellular scaffold of spinal cord and thermosensitive gel, which could continue to release exogenous basic fibroblast growth factor(BFGF) in the spinal lesion area on the basis of BFGF modified thermosensitive gel. Meanwhile, the porosity, pore size and material of the gray matter and white matter regions were distinguished by an isolation layer, so as to induce the directed differentiation of cells into the defect site and promote regeneration of spinal cord tissue. | Yang Liu Qian Li Bin Zhang De-Xiang Ban Shi-Qing Feng | 2017 | World Journal of Experimental Medicine2017，7，3： | 3 |
| 8 | Effect of basic fibroblast growth factor on cat corneal endothelial cell proliferation显示文摘AIM: To investigate the function of basic fibroblast growth factor (bFGF) on cat corneal endothelial cells proliferation. METHODS: Cat corneal endothelial cells were primarily cultured, stimulated with bFGF for different period, the proliferation of cells was assayed by modified tertrozalium salt (MTT) method, and the morphologic changes were observed with inverted phase contrast microscope and transmission electron microscope. RESULTS: At 1, 3 and 5 days after bFGF was added to cat corneal endothelial cells, the result of MTT in 490nm showed significant difference than that in control group, and the difference was most significant in 10ng/mL group. CONCLUSION: bFGF can promote proliferation of cat corneal endothelial cells. 10ng/mL is the relatively most effective dose. | Wen-Juan Luo Yang Zhou Mei-Guang Liu and Chuan-Fu Wang | 2011 | International Journal of Ophthalmology(English edition)2011，4，4： | 2 |
| 9 | Altered molecular pattern of mucosal healing in Crohn's disease fibrotic stenosis显示文摘AIM: To investigate tumor necrosis factor-α (TNF-α), syndecan 1 and basic fibroblast growth factor (bFGF) balance in Crohn's disease (CD) strictures. METHODS: Our study was performed on 24 surgical specimens of CD fibrotic stenosis. Ten histological normal surgical samples were retrieved for both the large and small bowel from patients with benign conditions and healthy tissue represented control collection. Sex and age in controls did not differ from CD group. Three endoscopic biopsy specimens taken after informed consent in subjects with normal colon were also used as negative controls. TNF-α, syndecan 1 and bFGF were detected by both reverse transcriptase reverse transcriptase polymerase chain reaction after mRNA extraction (results expressed as fold-change) and immunohistochemistry.RESULTS: TNF-α did not show any significant difference between CD and control specimens (1.54 ± 1.19; P > 0.05). Very high levels of bFGF were observed in CD (11.76 ± 4.65; P < 0.001) unlike syndecan 1 which showed a moderate increase (5.53 ± 2.18; P < 0.005). analysis of variance (ANOVA) plus Student-NeumannKeuls showed: bFGF > syndecan 1 > TNF-α = control. Immunoreactivity for bFGF was observed in epithelial, stromal, endothelial cells and even in the muscular layer, whilst in normal tissue it was almost unexpressed. Syndecan 1 and TNF-α staining was confined to mucosal epithelial and stromal cells, while in controls syndecan 1 was found in its normal site, i.e. , basolateral area of the crypts and TNF-α very poorly expressed. CONCLUSION: Fibrotic stenosis of CD may be the final result of an irreversible transformation of different cells into fibrogenic phenotype no longer inhibited by posttranscriptional regulation. | Enzo Ierardi Floriana Giorgio Domenico Piscitelli Mariabeatrice Principi Santina Cantatore Maria Grazia Fiore Roberta Rossi Michele Barone Alfredo Di Leo Carmine Panella | 2013 | World Journal of Gastrointestinal Pathophysiology2013，4，3： | 2 |
| 10 | 在煽动性的肠疾病的 Fibrogenesis 和纤维变性: 一样的硬币的好、坏的方面?显示文摘 Fibrogenesis in inflammatory bowel diseases is a complex phenomenon aimed at mucosal repair. However, it may provoke intestinal fibrosis with the development of strictures which require surgery. Therefore, fibrogenesis may be considered as a 'two-faced' process when related to chronic intestinal inflammation. Many types of cells may be converted into the fibrogenic phenotype at different levels of the intestinal wall. A complex interaction of cytokines, adhesion molecules and growth factors is involved in the process. We report an overview of recent advances in molecular mechanisms of stricturizing Crohn’s disease(CD) including the potential role of trasforming growth factor beta, protein kinase C and Ras, Raf and ERK proteins. Fibrotic growth factors such as vascular endothelial growth factor and platelet-derived growth factor, as well as the Endothelial-to-Mesenchymal Transition induced by transforming growth factor-β, are considered. Finally, our experience, focused on tumor necrosis factor α(the main cytokine of inflammatory bowel diseases) and the link between syndecan 1(a heparan sulphate adhesion molecule) and basic fibroblast growth factor(a strong stimulator of collagen synthesis) is described. We hypothesize a possible molecular pattern for mucosal healing as well as how its deregulation could be involved in fibrotic complications of CD. A final clinical point is the importance of performing an accurate evaluation of the presence of fibrotic strictures before starting anti-tumor necrosis α treatment, which could worsen the lesions. | Mariabeatrice Principi Floriana Giorgio Giuseppe Losurdo Viviana Neve Antonella Contaldo Alfredo Di Leo Enzo Ierardi | 2013 | World Journal of Gastrointestinal Pathophysiology2013，4，4： | 2 |
| 11 | Construction and Expression of Recombinant Plasmid pCD-rbFGF in Osteoblasts显示文摘Summary: To construct basic fibroblast growth factor (bFGF) eukaryotic expression vector and to evaluate the possibility of bFGF gene therapy in orthopedic disease, the pCD-rbFGF recombinant plasmid was constructed by cloning rat basic fibroblast growth factor (bFGF) cDNA into an eukaryotic expression vector, pcDNA 3. Rat osteoblasts were transfected with pCD-rbFGF plasmid by lopofectin mediated gene transfer, the transient expression was detected by streptavidin-biotin-enzyme complex (SABC) method. It was observed that the expression of rat bFGF gene was detected 72 h after transfected distinctly. Basic fibroblast growth factor gene therapy is a method of potential for a wide array of orthopedic diseases. | 杨操 杨述华 郭晓东 屈伸 | 2002 | Journal of Huazhong University of Science and Technology(Medical Sciences)2002，22，2： | 0 |
| 12 | EXPRESSION AND SIGNIFICANCE OF BASIC FIBROBLAST GWOWTH FACTOR AND FIBROBLAST GROWTH FACTOR RECEPTOR-1 IN OVARIAN EPITHELIAL NEOPLASM显示文摘Objective To study the relevance of expression of basic fibroblast growth factor (bFGF), fibroblast growth factor receptor 1 (FGFR 1) and carcinogenesis and progression of ovarian epithelial neoplasm. Methods Ten cases of normal ovarian tissues and 75 cases of ovarian epithelial neoplasm tissues were detected by immunohistochemical methods: S P for bFGF, FGFR 1,double immunohistochemistry Lab SA for Ki 67 antigen and bFGF. Results The expression level of bFGF, FGFR 1in ovarian epithelium and ovarian epithelial neoplasm showed a step wise increase in the following order:normal | 高尚风 杨蓉 高博 刘惠喜 | 2003 | Journal of Pharmaceutical Analysis2003，15，1： | 0 |
| 13 | The Effect of Hypoxia on Expression of Basic Fibroblast Growth Factor in Pulmonary Vascular Pericytes显示文摘To examine whether hypoxia exerts effect on the expression of basic fibroblast growth ac- tor (bFGF) in pulmonary vascular pericytes (PC), cell culture, in .citu hybridization with probe of digoxigenin-11-dUTP-labled cDNA, immunocytochemistry and image analysis were employed in this study. The results showed that the expression amount of bFGF mRNA and protein in PC of hypoxia (H) group was 1.31 times (P<0. 01) and 1. 17 times (P<0. 01) that of normoxia (N) group re- spectively. It suggests that hypoxia can directly enhance the expression of bFGF mRNA and protein in PC. Increased expression of bFGF may play an important role in the process of PC proliferation and differentiation of PC into smooth muscle-like cells. | 王林 熊密 车东媛 刘绍春 郝春荣 郑晓静 | 2000 | Journal of Huazhong University of Science and Technology(Medical Sciences)2000，20，4： | 0 |
| 14 | An in vivo Study of Basic Fibroblast Growth Factor on Activation and Proliferation of Retinal Progenitor Cells in RCS Rat显示文摘Purpose: To investigate the effect of intravitreal injection of basic fibroblast growth factor(bFGF) on activation and proliferation of endogenous retinal progenitor cells in the Royal College of Surgeons(RCS) rat. Methods: Twenty-four rats were studied after the 30th postnatal day(≥30). Eighteen RCS-p+/LAV rats were divided into 3 groups: bFGF-treated, vehicle-treated and untreated groups randomly, and 6 RCS-ray+p+/Lav respectively rats were used as normal controls. 6 μl of bFGF (5 μg/10 μl) or vehicle was injected into the vitreous on day 31, 33 and 35 after birth (P31, P33, P35) in the bFGF group and vehicle group respectively, and no injections were administered in the untreated and control groups. All the rats were euthanized, and their eyes were enucleated, hemisected and fixed at 50 d ays after birth for immunohistochemistry and measurement of outer nuclear layer thickness. Results:Nestin and Chx10 were positive in all retinal layers, intravitreal injection of bFGF in retina-dystrophic RCS(RCS-p+/Lav) rats induced intense labeling for the retinal progenitor cell markers Chx10 and Nestin, which were highly colocalized. Fluorescence intensity for both labels was somewhat less in the control rats, and much less in the vehicle-injected rats as well as in the untreated RCS rats. The outer nuclear layer(ONL) was significantly thicker in bFGF group than that in vehicle-treated or untreated group(P<0.01), but thinner than that of the control group(P<0.01). No significant difference was observed in the ONL thickness between the vehicle group and untreated group(P>0.05). Conclusion:bFGF may contribute to the activation of retinal progenitor cells in RCS rats,thus counteract degeneration by promoting the proliferation of the progenitor cells. | Xiaoping Xia Guoxiang Song Xiangfu Liu Xiangchen Tang Hui Ye | 2010 | Eye Science2010，25，2： | 0 |
| 15 | LMWH inhibits anterior chamber inflammation after extra capsular lens extraction through down regulation of bFGF content in aqueous humor显示文摘·AIM:To observe the changes of basic fibroblast growth factor (bFGF) content in anterior chamber before and after extra capsular lens extraction for investigating the mechanism of low molecular weight heparin (LMWH) inhibiting anterior chamber inflammation.·METHODS:Eighty-four rabbits were randomly divided into control and experimental group, 42 rabbits in each group. Extra capsular lens extraction was done on unilateral eye in each rabbit. LMWH was perfused into anterior chamber by the concentration of 50U/mL at the end of operation in experimental group. The degrees of corneal edema, aqueous flare and fibrin were evaluated with slit lamp microscope on postoperative day 1, 3, 6, 15, 30, 45 and 60, respectively. Six eyes of each group were at each time point. Contents of bFGF in aqueous humor were determined by ELISA after animals were killed. Another six eyes were used for determining the base line level of bFGF in aqueous humor.·RESULTS:The degrees of corneal edema, aqueous flare and fibrin in experimental group were significantly lighter than those in control group (P<0.01) on postoperative day 1, 3 and 6, respectively. No difference was showed between the two groups at other point time. Contents of bFGF in aqueous humor increased at the same time. bFGF content was reached peak on postoperative day 1 in experimental group, while on postoperative day 6 in control group. Contents of bFGF in the two groups declined slowly after reaching peak. The bFGF content in control group were significantly higher than that in experimental group 1-30 days after surgery (P < 0.05). No significant differences were shown between the two groups on postoperative day 45 and 60, respectively.·CONCLUSION:Perfusion with LMWH by the concentration of 50U/mL can significantly reduce anterior chamber inflammation after extra capsular lens extraction in rabbits, which may be related to down regulation of bFGF content in aqueous humor. | Jian-Ming Wang Lei Xiong Quan-Chen Xiong and Ya-Zhi Fan | 2012 | International Journal of Ophthalmology(English edition)2012，5，4： | 0 |
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