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| 1 | Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface显示文摘A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would | Langchong He Guangde Yang Xindu Geng | 1999 | Chinese Science Bulletin1999，44，9： | 58 |
| 2 | 超高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱快速筛查化妆品中的24种激素显示文摘建立了超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UPLC-LTQ/Orbitrap MS)快速筛查、定性识别化妆品中24种激素的分析方法。不同剂型的化妆品样品经甲醇超声提取,用Waters ACQUITY UPLC BEH C18色谱柱(50 mm×2.1 mm,1.7μm)分离,以乙腈和0.1%(v/v)甲酸水溶液为流动相进行梯度洗脱。通过静电场轨道阱全扫描得到激素化合物的准分子离子的精确质量数,实现对化妆品中激素的快速筛查;再以保留时间和数据依赖扫描(data dependent scan)模式获得的子离子质谱图进行定性确证。24种激素化合物的质量准确度误差小于3×10-6(3 ppm);线性良好,相关系数大于0.99;检出限≤10μg/kg(S/N=3),能满足实际化妆品样品的分析要求。应用该方法对不同剂型的50余种化妆品样品进行筛查分析,结果良好。该方法是化妆品中激素快速筛查、定性识别的有效方法。 | 李兆永 王凤美 牛增元 罗忻 张罡 陈军辉 | 2014 | 色谱2014，32，5： | 33 |
| 3 | Progress of gastric cancer etiology:N-nitrosamides in the 1990s显示文摘INTRODUCTIONStomach carcinoma is still the leading cause of cancer deathin China and the second one in the world.Its possiblecauses include:A)chemical factors such as intragastricformation of N-nitroso compounds(NOC)and high saltintake;B)biological factors such as infection | Da Jun Deng Laboratory of Cancer Etiology,Beijing Medical University School of Oncology& Beijing Institute for Cancer Research,100034,China | 2000 | World Journal of Gastroenterology2000，6，4： | 27 |
| 4 | Combined serum hepatoma-specific alpha-fetoprotein and circulating alpha-fetoprotein-mRNA in diagnosis of hepatocellular carcinoma显示文摘BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, its prognosis is poor, and early detection is of utmost importance. Although alpha-fetoprotein (AFP) is a useful marker for detecting and monitoring HCC development, the false-negative or false-positive rates with AFP alone may be as high as 30%-40% for patients with small HCCs. To enhance the specificity and accuracy of AFP measurements for HCC, we analyzed AFP expression states in livers, detected the hepatoma-specific AFP (HS-AFP) fraction and AFP-mRNA from peripheral blood mononuclear cells, and explored their clinical implications for HCC diagnosis. METHODS: AFP expression and hepatocyte distributions in liver specimens were investigated by an immunohistoche- mical assay. Total RNAs were extracted from circulating blood, synthesized to cDNA through random primers and reverse transcriptase, and fragments of the AFP gene were amplified by a nested-PCR assay. The HS-AFP fraction was separated by lectin-affinity chromatography and its level was detected by radioimmunoassay. RESULTS: The incidence of AFP was 73.3% in HCC tissues and its expression in HCCs with moderate or low differentiation was significantly stronger than that of HCCs with high differentiation (P<0.05). The incidence of AFP gene fragments was 100% in HCCs, and 60% in paracancerous tissues (P<0.01). In the HCC and liver cirrhosis groups, the incidence of HS-AFP was 91.7% and 18% (P<0.01), and of AFP-mRNA was 56.7% and 16% (P<0.01), respectively, and neither was found in controls.HS-AFP or AFP-mRNA was not significantly related to size or number of HCC, but to its differentiation, metastasis, and relapse (P<0.05). CONCLUSIONS: Different AFP expression is present in different parts of HCC tissues. HS-AFP and AFP-mRNA fragments improve sensitivity and specificity, and both are useful markers to diagnose HCC or monitor metastasis and relapse. | Wei Wu, Deng-Fu Yao, Yong-Mei Yuan, Ji-Wei Fan, Xiu-Feng Lu, Xiao-Hua Li, Li-Wei Qiu, Lei Zong and Xin-Hua Wu Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，4： | 26 |
| 5 | 加速溶剂萃取-高效液相色谱串联质谱法测定土壤中农药残留显示文摘1引言土壤中农药残留可分为杀虫剂、除草剂和杀菌剂残留。常见杀虫剂有克百威和甲氰菊酯等,常见除草剂有苯噻酰草胺、乙草胺、丙草胺、喹禾灵和草酮等,常见杀菌剂有烯酰吗啉和稻瘟灵等。克百威是广谱性杀虫剂,它与胆碱酯酶结合不可逆,因此毒性很高;乙草胺是使用量极大的除草剂之一。这两种农药的毒性远高于其它几种常见农药。快速的前处理及稳定的仪器检测是研究土壤中农药残留的关键,尤其是各类型农药的同时检测,不仅可以提高检测效率。 | 闫蕊 邵明媛 鞠福龙 宋大千 张寒琦 于爱民 | 2013 | 分析化学2013，41，2： | 26 |
| 6 | Imperatorin Is Responsible for the Vasodilatation Activity of Angelica Dahurica var. Formosana Regulated by Nitric Oxide in an Endothelium-Dependent Manner显示文摘Objective:To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica var.formosana and to further analyze active components in the herb extracts.Methods:(1) The vasodilatation effects induced by different extracts(cyclohexane,ethyl acetate,acetone,methanol,95%ethanol and water) of Angelica dahurica var.formosana on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-per... | 聂红 孟兰贞 周玖瑶 樊晓峰 罗勇 张广文 | 2009 | Chinese Journal of Integrative Medicine2009，15，6： | 17 |
| 7 | 柱前衍生高效液相色谱法同时测定水性涂料中6种醛类化合物显示文摘建立了柱前衍生高效液相色谱同时测定水性涂料中甲醛、乙醛、丙醛、苯甲醛、正戊醛和对甲基苯甲醛等6种醛类化合物的方法。样品经水超声提取后,与2,4-二硝基苯肼乙腈溶液在酸性条件下衍生,再经0.45μm 针式过滤器过滤后进样分析。系统考察了酸度调节剂、pH 值、反应温度、时间等因素对衍生反应的影响,优化的反应条件为:以稀释后的盐酸溶液作为酸度调节剂,缓冲溶液 pH =3,反应温度为60℃,反应时间为30 min。在此条件下,于0.08~2.0 mg / L 浓度范围内,6种醛类化合物呈良好的线性关系;检出限为0.05~2.50 mg / kg;在2.0、4.0、6.0 mg / kg 3个水平下的加标回收率为87.0%~112.8%;RSD 为1.12%~9.54%。结果表明,本方法具有较宽的线性范围,良好的精密度和准确度,适用于水性涂料中6种醛类化合物的同时测定。 | 陈潜 周宇艳 程欲晓 林苗 | 2014 | 色谱2014，32，3： | 14 |
| 8 | Study on the Relationship between Plasma Homocysteine and Acute Cerebral Vascular Disease显示文摘The levels of plasma homocysteine were determined by using high-performance liquid chromatographic method. It was found that plasma homocysteine levels were significantly higher in the patients with stroke than that in the controls. There was no correlation between plasma homocys- teine levels and hypertension, smoking, concentrations of blood glucose or hypertriglyceridesemia. It was suggested that hyperhomocysteinemia may be an independent risk factor for acute cerebral vascu- lar disease. | 彭海 黄谦 李月芬 孙圣刚 邓学军 乔娴 | 2000 | Journal of Huazhong University of Science and Technology(Medical Sciences)2000，20，4： | 11 |
| 9 | Metabolomics of gastric cancer metastasis detected by gas chromatography and mass spectrometry显示文摘AIM:To elucidate the underlying mechanisms of metastasis and to identify the metabolomic markers of gastric cancer metastasis.METHODS:Gastric tumors from metastatic and nonmetastatic groups were used in this study.Metabolites and different metabolic patterns were analyzed by gas chromatography,mass spectrometry and principal components analysis (PCA),respectively.Differentiation performance was validated by the area under the curve (AUC) of receiver operating characteristic curves.RESULTS:Twenty-nine metabolites were differentially expressed in animal models of human gastric cancer.Of the 29 metabolites,20 were up-regulated and 9 were down-regulated in metastasis group compared to non-metastasis group.PCA models from the metabolite profiles could differentiate the metastatic from the nonmetastatic specimens with an AUC value of 1.0.These metabolites were mainly involved in several metabolic pathways,including glycolysis (lactic acid,alaline),serine metabolism (serine,phosphoserine),proline metabolism (proline),glutamic acid metabolism,tricarboxylic acid cycle (succinate,malic acid),nucleotide metabolism (pyrimidine),fatty acid metabolism (docosanoic acid,and octadecanoic acid),and methylation(glycine).The serine and proline metabolisms were highlighted during the progression of metastasis.CONCLUSION:Proline and serine metabolisms play an important role in metastasis.The metabolic profiling of tumor tissue can provide new biomarkers for the treatment of gastric cancer metastasis. | Jin-Lian Chen,Jun-Duo Hu,Jing Fan,Jing Hong,Department of Gastroenterology,Shanghai Sixth People’s Hospital,Shanghai Jiao Tong University,Shanghai 200233,China Hui-Qing Tang,Jian-Zhong Gu,Shanghai Laboratory Animal Center,Chinese Academy of Sciences,Shanghai 201615,China | 2010 | World Journal of Gastroenterology2010，16，46： | 11 |
| 10 | 超临界色谱法测定化妆品中生育酚及α-生育酚乙酸酯显示文摘建立了化妆品中4种生育酚异构体及α-生育酚乙酸酯的超临界色谱分析方法.不同基质类型的化妆品经乙腈水溶液-正己烷溶剂体系按不同的添加顺序进行分散、提取、液液分配后,以二氧化碳为流动相,异丙醇-正己烷(1∶1)为改性剂,利用亚乙基桥杂化颗粒色谱柱(Viridis@BEH,250 mm×4.6 mm,5μm)对目标物和样品杂质进行有效分离,用光电二极管矩阵检测器(SFC-PDA)进行测定.系统考察了提取条件和色谱条件对实验结果的影响.结果表明:4种生育酚和α-生育酚乙酸酯的定量下限(S/N=10)分别为50 mg/kg和25 mg/kg,空白基质加标回收率为81.3%~ 109.5%,相对标准偏差(RSD)不大于12.8%.该方法前处理简单、快速、准确,可用于各类化妆品中4种生育酚异构体及α-生育酚乙酸酯含量的检测. | 李慧勇 席绍峰 谭建华 王继才 熊小婷 赵田甜 吴楚森 郭长虹 | 2014 | 分析测试学报2014，33，4： | 10 |
| 11 | Distribution and significance of C~_(40) alkanes in the extracts of Cretaceous source rocks from the Songliao Basin显示文摘Source rock extracts and crude oils from the Songliao Basin were analyzed by high-temperature gas chromatography (HTGC), gas chromatography-mass spectrometry (HTGC-MS) and gas chromatography-isotope ratio-mass spectrometry (GC-IRMS), for high molecular-weight alkanes. The distributions of n-alkanes in the Nenjiang Formation extracts are in the C14―C63 range; a bimodal distribution occurs in the C-21 and C21―40 regions. The C30―C37 n-alkanes are accompanied by C29―C35 hopanes, whereas the high molecular-weight C45―C47 n-alkanes co-occur with abundant isoalkanes, alkylcyclohexanes and alkylcyclopentanes. The high δ 13C values of the n-alkanes and the microscopic maceral compositions indicate a highly diversified organic source input for the Nenjiang Formation source rocks, ranging from aquatic plants, blue alge-bacteria, to land plant material. In contrast, n-alkanes in the rock extracts of the Qingshankou Formation are characterized by a single modal distribution, with relatively low abundances of C29―C35 hopanes, but high molecular-weight isoalkanes, alkylcyclohexanes and alkylcyclopentanes. The relatively low δ 13C values of C22―C44 n-alkanes and organic material compositions indicate that the source rocks in the Qingshankou Formation contain dominantly type I algal organic matter. The relative abundance of C+40 compounds in source rocks changes little at low maturity stage, but decreases drastically at higher maturity levels, with a concurrent reduction in the odd/even carbon predominance. In crude oils, in contrast, the relative abundance of C+40 compounds appears to relate closely with the oil source and oil viscosity. | FENG ZiHui FANG Wei ZHANG JuHe LI ZhenGuang HUANG ChunYan WANG Xue ZHAO QinLing HUO QiuLi | 2007 | Science China Earth Sciences2007，50，10： | 10 |
| 12 | 凝胶渗透色谱-高效液相色谱-线性离子阱质谱法测定膳食样品中的氨基甲酸酯类农药残留显示文摘建立了高效液相色谱-线性离子阱质谱( HPLC-LIT-MS)测定膳食样品中氨基甲酸酯类农药的检测方法。样品中加入同位素内标,用正己烷饱和的乙腈超声提取,凝胶渗透色谱净化。以乙腈和含5 mmol/L 甲酸铵和0.1%(v/v)甲酸的水溶液为流动相,目标化合物经 CAPCELL PAK CR(100 mm×2.1 mm,5μm;SCX-C18(1:4))色谱柱分离,梯度洗脱,流速0.2 mL/min。采用电喷雾电离,选择反应监测( SRM)正离子模式三级离子监测。内标法定量。膳食类样品中氨基甲酸酯农药的平均加标回收率在60.4%~114%之间;相对标准偏差在3.46%~16.2%范围内;检出限( LODs)在0.001~0.010 mg/kg之间。应用所建立的方法对2007年第四次中国总膳食研究项目的9类膳食样品基质中的氨基甲酸酯类农药进行了检测,在少量样品中检出了涕灭威和克百威。 | 杨欣 李鹏 苗虹 赵云峰 吴永宁 | 2014 | 色谱2014，32，5： | 9 |
| 13 | Deeper Chemical Perceptions for Better Traditional Chinese Medicine Standards显示文摘Traditional Chinese medicines (TCMs), a complex system of natural resources with many diverse components, are widely used as approved medicinal agents in China. Quality control of TCMs is a huge challenge for the government and for testing institutes and is associated with numerous scientific issues. Among these considerations include the following questions: How many components are in TCMs? How can the multiple components in TCMs be comprehensively delineated and subsequently characterized? What is the level and range of these (active) metabolites within these multiple-component TCMs, in order to recommend standards? and What are the qualities required for a marker constituent to be selected, and from a practical perspective, how can these components be assessed with low cost and in a short time? All of these factors require significant and deep thinking in order to understand the individualistic chemistry of TCM in order to develop enhanced TCM quality standards for improved and consistent patient care. In this review, the latest exploratory research in TCM chemistry analytical techniques and methods is summarized in order to begin to develop responses to these scientific issues. Advances in these methods have included multidimensional separation for liquid chromatography–high-resolution mass spectrometry (LC–HRMS), smart triggering data-dependent acquisition of LC–HRMS, target analysis with liquid chromatography–mass spectrometry (LC–MS), supercritical fluid chromatography, and data mining of large mass spectrometry (MS) datasets. In addition, two quality strategies have been introduced in order to save reference standards and the analysis time for a TCM quality standard, including the application of the single standard to determine multi-components (SSDMC) and monomethodheterotrait matrix methods. Finally, a series of future improvements for analytical methods for TCMs are proposed. | Jin-Jun Hou Jian-Qing Zhang Chang-Liang Yao Rudolf Bauer Ikhlas A. Khan Wan-Ying Wu De-an Guo | 2019 | Engineering2019，5，1： | 9 |
| 14 | Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling fourdimensional separation and characterization of the multicomponents from white ginseng and red ginseng显示文摘Inherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification.A dimension-enhanced strategy,by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(2 D-LC/IM-QTOF-MS)enabling four-dimensional separations(2 D-LC,IM,and MS),is proposed.In combination with in-house database-driven automated peak annotation,this strategy was utilized to characterize ginsenosides simultaneously from white ginseng(WG)and red ginseng(RG).An offline 2 DLC system configuring an Xbridge Amide column and an HSS T3 column showed orthogonality 0.76 in the resolution of ginsenosides.Ginsenoside analysis was performed by data-independent high-definition MSE(HDMSE)in the negative ESI mode on a Vion?IMS-QTOF hybrid high-resolution mass spectrometer,which could better resolve ginsenosides than MSEand directly give the CCS information.An in-house ginsenoside database recording 504 known ginsenosides and 58 reference compounds,was established to assist the identification of ginsenosides.Streamlined workflows,by applying UNIFI?to automatedly annotate the HDMSEdata,were proposed.We could separate and characterize 323 ginsenosides(including 286 from WG and 306 from RG),and 125 thereof may have not been isolated from the Panax genus.The established 2 D-LC/IM-QTOF-HDMSEapproach could also act as a magnifier to probe differentiated components between WG and RG.Compared with conventional approaches,this dimensionenhanced strategy could better resolve coeluting herbal components and more efficiently,more reliably identify the multicomponents,which,we believe,offers more possibilities for the systematic exposure and confirmative identification of plant metabolites. | Tiantian Zuo Chunxia Zhang Weiwei Li Hongda Wang Ying Hu Wenzhi Yang Li Jia Xiaoyan Wang Xiumei Gao Dean Guo | 2020 | Journal of Pharmaceutical Analysis2020，10，6： | 9 |
| 15 | 一株产紫杉醇的曼地亚红豆杉内生真菌的分离及鉴定(摘要)(英文)显示文摘[目的]分离并鉴定1株产紫杉醇的曼地亚红豆杉内的内生真菌。[方法]从曼地亚红豆杉树皮内表皮中分离得到32株内生真菌,并通过高效液相色谱法检测其发酵产物。[结果]筛选获得1株可以产紫杉醇的内生真菌M57,其紫杉醇产量为45~50μg/L,并通过对M57菌落的形态学观察以及18S rDNA序列分析初步将其鉴定为根霉属(Rhizopus)真菌。[结论]该菌株的发现为微生物发酵法生产紫杉醇提供了具有潜在应用价值新的菌种。 | 李佟清 张志建 张鹏 王春兰 刘博 刘婷婷 付春华 余龙江 | 2010 | Agricultural Science & Technology2010，11，5： | 8 |
| 16 | 基于GC-FID、HS-SPME-GC-MS与电子鼻技术评价不同水果发酵酒的香气特征显示文摘采用气相色谱-氢火焰离子化检测器法(gas chromatography with hydrogen flame ionization detection,GCFID)、顶空固相微萃取-气相色谱-质谱(headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry,HS-SPME-GC-MS)和电子鼻技术分析6种果酒中的挥发性成分,评价果酒风味轮廓特征的差异性。结果表明,GC-FID定量测定樱桃李酒,其中异戊醇、活性戊醇、β-苯乙醇含量最高,乙酸乙酯含量最低;木瓜酒中正丙醇、2,3-丁二醇、癸酸乙酯、乙酸含量最高; 3-羟基-2-丁酮含量在樱桃李酒中最高,山楂酒、甜橙酒中最少。HP-SPME-GC-MS鉴定果酒中挥发性物质94种,其中醇类23种,酯类43种,醛酮类10种,酸类8种,酚类2种,苯环类5种,烷烃类3种。山楂酒、菠萝酒、木瓜酒、甜橙酒、无花果酒和樱桃李酒中香气物质数量分别为34、44、45、45、33、47种。其中山楂酒、木瓜酒和无花果酒中未检出酚类、烷烃类物质。电子鼻对不同果酒香气的区分效果无重叠,W5S传感器区分果酒香气能力最强。比较而言,菠萝酒和樱桃李酒的口感更圆润,香气更愉悦,感官得分最高。 | 徐康 路遥 宋英珲 张召全 郭萌萌 | 2018 | 食品与发酵工业2018，44，12： | 8 |
| 17 | Simultaneous determination of amino acids in different teas using supercritical fluid chromatography coupled with single quadrupole mass spectrometry显示文摘Tea is a widely consumed beverage and has many important physiological properties and potential health benefits. In this study, a novel method based on supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) was developed to simultaneously determine 11 amino acids in different types of tea (green teas, Oolong tea, black tea and Pu-erh tea). The separation conditions for the analysis of the selected amino acids including the column type, temperature and backpressure as well as the type of additive, were carefully optimized. The best separation of the 11 amino acids was obtained by adding water (5%, v/v) and trifluoroacetic acid (0.4%, v/v) to the organic modifier (methanol). Finally, the developed SFC-MS method was fully validated and successfully applied to the determination of these amino acids in six different tea samples. Good linearity (r ≥ 0.993), precision (RSDs≤ 2.99%), accuracy (91.95%-107.09%) as well as good sample stability were observed. The limits of detection ranged from 1.42 to 14.69 ng/mL, while the limits of quantification were between 4.53 and 47.0 ng/mL. The results indicate that the contents of the 11 amino acids in the six different tea samples are greatly influenced by the degree of fermentation. The proposed SFC-MS method shows a great potential for further investigation of tea varieties. | Yang Huang Tiejie Wang Marianne Fillet Jacques Crommen Zhengjin Jiang | 2019 | Journal of Pharmaceutical Analysis2019，9，4： | 7 |
| 18 | 分散液液微萃取-反相液液微萃取-扫集-胶束电动色谱法测定红酒中的3种氯酚类物质显示文摘建立了分散液液微萃取(DLLME)-反相液液微萃取(RP-LLME)-扫集-胶束电动色谱富集模型,并用于红酒中五氯酚(PCP)、2,4,6-三氯酚(TCP)和2,4-二氯酚(DCP)3种氯酚的测定。实验考察了两步微萃取的萃取参数对氯酚萃取率的影响和样品分离富集的电泳条件。最佳萃取条件 DLLME 为:3.5 mL 红酒( pH 3.0,120 g / L NaCl),300μL 正己烷(萃取剂);RP-LLME 为:25μL 0.16 mol / L NaOH(萃取剂)。最佳电泳条件:25 mmol / L NaH2 PO4,100 mmol / L 十二烷基硫酸钠( SDS),30%( v / v)乙腈,pH 2.3;分离电压-15 kV;样品基质为80 mmol / L NaH2 PO4;压力进样20 s×20.67 kPa(3 psi)。PCP 和 TCP 的线性范围为0.5~100μg / L( r≥0.9910), DCP 的线性范围为1.5~80μg / L(r =0.9851)。3种分析物的检出限( S / N =3)为0.035~0.114μg / L,加标回收率为75.2%~104.7%,相对标准偏差≤6.17%。该方法富集倍数高、灵敏度高、重现性好、分析速度快,可为不同样品基质中痕量氯酚污染物及某些弱酸性有机污染物测定提供参考。 | 孙建芝 贺晖 刘书慧 | 2014 | 色谱2014，32，3： | 7 |
| 19 | Mutations of beta-amyloid precursor protein alter the consequence of Alzheimer's disease pathogenesis显示文摘Alzheimer's disease is pathologically defined by accumulation of extracellular amyloid-β(Aβ). Approximately 25 mutations in β-amyloid precursor protein(APP) are pathogenic and cause autosomal dominant Alzheimer's disease. To date, the mechanism underlying the effect of APP mutation on Aβ generation is unclear. Therefore, investigating the mechanism of APP mutation on Alzheimer's disease may help understanding of disease pathogenesis. Thus, APP mutations(A673T, A673 V, E682 K, E693 G, and E693Q) were transiently co-transfected into human embryonic kidney cells. Western blot assay was used to detect expression levels of APP, beta-secretase 1, and presenilin 1 in cells. Enzyme-linked immunosorbent assay was performed to determine Aβ_(1–40) and Aβ_(1–42) levels. Liquid chromatography-tandem mass chromatography was used to examine VVIAT, FLF, ITL, VIV, IAT, VIT, TVI, and VVIA peptide levels. Immunofluorescence staining was performed to measure APP and early endosome antigen 1 immunoreactivity. Our results show that the protective A673 T mutation decreases Aβ_(42)/Aβ_(40) rate by downregulating IAT and upregulating VVIA levels. Pathogenic A673 V, E682 K, and E693 Q mutations promote Aβ_(42)/Aβ_(40) rate by increasing levels of CTF99, Aβ_(42), Aβ_(40), and IAT, and decreasing VVIA levels. Pathogenic E693 G mutation shows no significant change in Aβ_(42)/Aβ_(40) ratio because of inhibition of γ-secretase activity. APP mutations can change location from the cell surface to early endosomes. Our findings confirm that certain APP mutations accelerate Aβ generation by affecting the long Aβ cleavage pathway and increasing Aβ_(42/40) rate, thereby resulting in Alzheimer's disease. | Nuo-Min Li Ke-Fu Liu Yun-Jie Qiu Huan-Huan Zhang Hiroshi Nakanishi Hong Qing | 2019 | Neural Regeneration Research2019，14，4： | 7 |
| 20 | 高效液相色谱法同时测定酱腌菜中防腐剂和甜味剂显示文摘建立了酱腌菜中安赛蜜、苯甲酸、山梨酸、糖精钠4种食品添加剂的高效液相色谱同时测定方法。采用C18色谱柱,甲醇+0.02 mol/L乙酸铵(4+96)为流动相,流速1.0 mL/min,柱温25℃,二极管阵列检测器,检测波长230 nm。结果表明:该方法4种添加剂回归方程线性关系良好,相对标准偏差在4.5%以下,回收率在89.6%~105.9%之间。该方法简便快速,准确可靠,可用于各类酱腌菜中安赛蜜、苯甲酸、山梨酸、糖精钠含量的同时测定。 | 吴晓红 李小华 宋佳 敖雪彦 | 2014 | 中国调味品2014，39，2： | 6 |
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