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您的检索式：关键字=cold acclimation
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| 1 | Cold acclimation improves photosynthesis by regulating the ascorbate–glutathione cycle in chloroplasts of Kandelia obovata显示文摘As the most northerly mangrove species in China, Kandelia obovata may undergo extreme cold event stress. Enhancing the cold tolerance of this species is crucial to its successful afforestation. This study aimed to determine the resistance of K. obovata seedlings to low temperature stress by cold acclimation and to explain the mechanisms for alleviating cold injury. To understand these mechanisms, seedlings that were acclimatized and not acclimatized were exposed to 5℃/- 2℃(day/night)for 48 h.Results showed that low temperature stress reduced leaf photosynthesis of non-acclimatized seedlings by inducing oxidative stress and structural damage to chloroplasts. These phenomena were shown by increasing levels of malondialdehyde (MDA), O2-and H2O2, as well as decreasing enzyme activities in the ascorbate–glutathione (AsA-GSH) cycle. However, cold-acclimatized seedlings had improved photosynthetic rates and efficiency of photosystem II (PSII) under low temperature stress. Compared with non-acclimatized seedlings, leaves of coldacclimatized seedlings under low temperature stress for 48 h exhibited higher anti-oxidative enzyme activities, lower levels of O2^- and H2O2, less damage to chloroplast structure, and removed 33.7% of MDA at low temperature stress for 48 h. The data indicate that cold acclimation enhances photosynthetic capacity by effectively regulating activation in the PSII electron transport and the AsA–GSH cycle to scavenge excess ROS in chloroplasts, while the latter is more important. | Weicheng Liu Chunfang Zheng Jinong Chen Jianbiao Qiu Zhixing Huang Qi Wang Yong Ye | 2019 | Journal of Forestry Research2019，30，3： | 11 |
| 2 | Functional analysis of cold-inducible cDNA clones in the legume Ammopiptanthus mongolicus显示文摘Ammopiptanthus mongolicus is the only evergreen broadleaved shrub endemic species to the Alashan Desert, northwest sand area of China, and can survive at -30℃ or even lower temperature in winter. A modified solid-phase subtraction hybridization technique was developed to isolate and screen cDNAs, whose transcripts increased in cold-treated A. mongolicus seedlings. Sequence analysis of the screened clones indicated that 11 clones had coding regions, with four of them containing complete open reading frame. And nine of the 11 clones shared various degrees of homology with the genes found in the GenBank database and the other two were unidentified sequences. Sequence data further revealed that these accumulated transcripts encode three low molecular weight proteins, a late-embryogenesis protein and two cold acclimation responsive proteins; two photosynthesis-related proteins, photosystem I subunit II precursor (PsaD) and photosystem II oxygen-evolving complex 33kDa subunit (OEC33); a protease inhibitor; an adenosine triphosphatase and a 14-3-3 related protein. Analysis on the function of these proteins indicated that the low molecular weight proteins were associated with water holding ability of cytoplasm; photosynthesis-related proteins participated in the adjustments of photosynthetic apparatus to resist photoinhibition; 14-3-3 related protein could interact with adenosine triphosphatase to enhance ATPase activity and energy metabolism; and protease inhibitor involved in the prevention of unwanted cell death was caused by reactive oxygen species. We suggest that cold acclimation with low light intensity in A. mongolicus is a more complex interaction of low temperature, light, energy and signal than that assumed previously. | LIU Mei-qin SHEN Xin YIN Wei-lun LU Cun-fu(College of Biological Sciences and Biotechnology, Beijing Forestry University) | 2006 | 北京林业大学学报2006，28，S2： | 1 |
| 3 | Changes of neuroleukin mRNA levels in hypothalamus pituitary and splenic lymphocytes of rats during cold acclimation显示文摘Neuroleukin(NLK),a protein(Mr 56000),acts on both the nervous and the im-mune systems and its expression is regulated at mRNA levels.One hundred fifty Sprague-Daw-ley(SD)male rats were divided into 5 groups:control group(N)and groups of cold-exposurefor 1,2,3 and 4 weeks(ClW,C2W,C3W and C4W).With RNA dot blot experiments,changes of NLK mRNA levels in both neuroendocrine system(pituitary and hypothalamus)andimmune system(splenic lymphocytes,LC)of SD male rats during cold acclimation presentedsimilar responses.C/N ratios of scan gray intensity level in RNA dots are presented as follows:there was a rise in C/N ratio to 1.63~11.31 in CIW,a fall to 0.63 in C2W and a rise again to1.42~21.09 in C3W.It might thus be regarded as a general response to cold surroundings.Itwas reported that SDS-PAGE autoradiogram of synthetic proteins in LC and secretive proteinsin supernatant cultured in vitro showed that NLK content in LC was not parallel to its mRNAlevel because NLK was extremely meager over the 1-week cold exposure whereas it increasedobviously over the 3-and 4-week cold exposure.This is probably a major cause of why im-munoglobulin synthesis in cold-acclimated animals was greatly enlarged. | 徐扬 杨泽田 苏成芝 | 1992 | Journal of Medical Colleges of PLA(China)1992，7，3： | 0 |
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