| 1 | LncRNA-XIST/miR-137/Notch1在糖尿病视网膜病变患者血清和玻璃体中的表达及机制显示文摘目的探讨lncRNA-XIST在糖尿病视网膜病变患者血清和玻璃体中的表达及其机制。方法收集糖尿病视网膜病变患者,同时检测血清和玻璃体中lncRNA-XIST和miR-137的表达量。运用高糖诱导的HRMECs作为体外模型。运用PCR、Western blot、CCK-8、ELISA和荧光素酶报告检查lncRNA-XIST对糖尿病视网膜病变的机制。结果血清和玻璃体中lncRNA-XIST表达量被抑制,miR-137表达量被激活,差异均有统计学意义(P<0.05)。激活lncRNA-XIST能够促进视网膜细胞增殖,减少ROS和MDA的水平,增加GSH、GSH-PX和SOD水平,差异均有统计学意义(P<0.05)。miR-137+lncRNA-XIST减少荧光素酶表达量,miR-137是LncRNA-XIST重要靶点。激活miR-137能够抑制视网膜细胞增殖,提高了ROS和MDA的水平,减少GSH、GSH-PX和SOD水平,差异均有统计学意义。miR-137+Notch1减少荧光素酶表达量,Notch1是miR-137重要靶点。结论lncRNA-XIST/miR-137/Notch1通过ROS及其氧化应激,能够阻止糖尿病视网膜病变,提示lncRNA-XIST可作为预测糖尿病视网膜病变的良好分子标志物。 | 许瑶 娄静 赵峰 | 2020 | 实用医学杂志2020，36，17： | 7 |
| 2 | Synthesis of taurine-fluorescein conjugate and evaluation of its retina-targeted efficiency in vitro显示文摘In this work, retinal penetration of fluorescein was achieved in vitro by covalent attachment of taurine to fluorescein, yielding the F-Tau conjugate. Nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS) were used to confirm the successful synthesis of F-Tau. The cellular uptake of F-Tau in adult retinal pigment epithelial cells (ARPE-19) and human retinal microvascular endothelial cells (hRMECs) was visualized via confocal scanning microscopy. The results indicated an improvement of solubility and a reduction of logP of F-Tau compared with fluorescein. As compared with fluorescein, F-Tau showed little toxicity, and was retained longer by cells in uptake experiments. F-Tau also displayed higher transepithelial permeabilities than fluorescein in ARPE-19 and hRMECs monolayer cells (Po0.05). These results showed that taurine may be a useful ligand for targeting small-molecule hydrophobic pharmaceuticals into the retina. | Meihong Huang Jiaqi Song Bingzheng Lu Huizhi Huang Yizhen Chen Wei Yin Wenbo Zhu Xinwen Su Chuanbin Wu Haiyan Hu | 2014 | Acta Pharmaceutica Sinica B2014，4，6： | 4 |
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