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| 1 | Gene diagnosis and targeted breeding for blast-resistant Kongyu 131without changing regional adaptability显示文摘The fungus Magnaporthe oryzae threatens the rice production of Kongyu 131 (KY131),a leading japonica variety in Northeast China.In this study,two rice lines,KP1 and KP2-Hd1,were obtained by introgressing the blast resistance genes Pi1 and Pi2 into KY131,respectively.However,both lines headed later than KY131.RICE60K SNP array analysis showed that Hd1 closely linked to Pi2 was introgressed into KP2-Hd1,and the linkage drag of Hd1 was broken by recombination.On the other hand,no known flowering genes were introgressed into KP1.Gene diagnosis by resequencing six flowering genes showed that KP1 carried functional Hd16 and Ghd8 alleles.Due to its suppression role in heading under long-day conditions,Ghd8 was chosen as the target for gene editing to disrupt its function.Four sgRNAs targeting different sites within Ghd8 were utilized to induce large-deletion mutations,which were easy to detect via agarose gel electrophoresis.All the ghd8-mutated KP1 lines were resistant to rice blast disease and headed earlier than the control KP1,even than KY131,under natural long-day conditions,which ensures its growth in Northeast China.This study confirmed that a combination of gene diagnosis and targeted gene editing is a highly efficient way to quickly eliminate undesired traits in a breeding line. | Xiangchun Zhou Gonghao Jiang Longwei Yang Lei Qiu Ping He Chunxiao Nong Yunyue Wang Yuqing He Yongzhong Xing | 2018 | Journal of Genetics and Genomics2018，45，10： | 7 |
| 2 | 小麦抽穗期基因研究进展显示文摘抽穗期是小麦品种的重要农艺性状之一,它对于小麦适应不同生态地区和不同环境条件具有至关重要的作用。春化反应、光周期反应和早熟性基因是影响小麦抽穗期的3种重要因素。综述了小麦抽穗期相关基因的定位和克隆,分析了控制小麦抽穗的3类基因性状以便为研究小麦的抽穗期基因提供参考。 | 董春林 张明义 张晓军 | 2010 | 江西农业学报2010，22，9： | 1 |
| 3 | Construction of a Contig Encompassing Ef(t) Gene Locus Using a Rice BAC Library显示文摘A major gene for heading date in rice, Ef(t), was mapped on the same position as one RFLP marker C1369 on chromosome 10, which was located between two RFLP markers C234 and G37 at 1.0 cM interval. Initially, these three RFLP markers were used to screen a rice BAC library and seven independent clones with the size ranged from 70kb to 180kb were identified. By the comparisons of Hind III restriction fragment in each clone, the relative location of these clones were determined and two primary contigs, contig C1369 and contig G37, were obtained. Chromosome walking was performed with one outmost BAC end of the primary BAC contig C1369, then two contigs were integrated into one. The resultant contig encompassing Ef(t) gene locus which consisted of 7 BAC clones was developed. It will facilitate the isolation of Ef(t) gene using map based cloning approach. | 曲雪萍 | 2000 | High Technology Letters2000，6，3： | 0 |
| 4 | Mapping of Hd-6-2 for Heading Date Using Two Secondary Segregation Populations in Rice显示文摘Heading date is one of the most important traits for rice adaption to different cultivation areas and crop seasons. In this study, two single segment substitution lines(SSSLs), W31-41-61-3-11-3-6-7(W31-SSSL) and W32-59-80-2-11-1-10(W32-SSSL) with substituted intervals derived from the donor parents IR66897 B(W31) and IR66167-27-5-1-6(W32), respectively, with Huajingxian 74(HTX74) were found to comprise a gene for extremely late-heading date, and the gene was tentatively designated as Hd-6-2. Two secondary F2 segregating populations were developed by crossing the two heterozygous SSSLs with HJX74 to map Hd-6-2 gene. According to phenotype analysis of the two mapping populations, the late heading date trait was controlled by a major recessive gene. In the segregation population derived from W31-SSSL, Hd-6-2 was mapped on chromosome 6 between PSM677 and RM204 with the genetic distances of 1.3 and 2.7 c M, respectively. In the population of W32-SSSL, the gene for heading date was mapped to the similar region as Hd-6-2 and co-segregated with PSM672. The sequence alignment of Hd3 a in the coding domains and promoter regions of HJX74 and W31-SSSL are completely consistent, whereas there was a great difference between W32-SSSL and HJX74, suggesting that Hd3 a could hardly be the main cause of the heading date variation in W31-SSSL, but it was probably the main reason for the change of heading stage in W32-SSSL. | ZHANG Hua LIU Xu YANG Yongyi XUAN Ning YAO Fangyin | 2018 | Rice science2018，25，3： | 0 |
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