|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A Liutex based definition and identification of vortex core center lines显示文摘Six core issues for vortex definition and identification concern with (1) the absolute strength,(2) the relative strength,(3) the rotational axis,(4) the vortex core center,(5) the vortex core size, and (6) the vortex boundary (Liu C. 2019). However, most of the currently popular vortex identification methods, including the Q criterion, the criterion and the Acj criterion etc., are Eulerian local region-type vortex identification criteria and can only approximately identify the vortex boundary by somewhat arbitrary threshold. On the other hand, the existing Eulerian local line-type methods, which seek to extract line-type features such as vortex core line, are not entirely satisfactory since most of these methods are based on vorticity or pressure minimum that will fail in many cases. The key issue is the lack of a reasonable mathematical definition for vortex core center. To address this issue, a Liutex (previously named Rortex) based definition of vortex core center is proposed in this paper. The vortex core center, also called vortex rotation axis line here, is defined as a line where the Liutex magnitude gradient vector is aligned with the Liutex vector, which mathematically implies that the cross product of the Liutex magnitude gradient vector and the Liutex vector on the line is equal to zero. Based on this definition, a novel three-step method for extracting vortex rotation axis lines is presented. Two test cases, namely the Burgers vortex and hairpin vortices, are examined to justify the proposed method. The results demonstrate that the proposed method can successfully identify vortex rotation axis lines without any user-specified threshold, so that the proposed method is very straightforward, robust and efficient. | Yi-sheng Gao Jian-ming Liu Yi-fei Yu Chaoqun Liu | 2019 | Journal of Hydrodynamics2019，31，3： | 19 |
| 2 | Expression of VEGF_(121)in gastric carcinoma MGC803 cell line显示文摘INTRODUCTIONVascular endothelial growth factor(VEGF)whichis also known as vascular permeability factor(VPF)is a heparin-binding,dimeric polypeptide growthfactor and a potent mitogen for endothelial cells.VEGF can stimulate the endothelial cell growth andenhance the motility through its two knownreceptors flt-1 and KDR.Acting through thesereceptors,VEGF may stimulate angiogenesis | Xue Jun Tian Jian Wu Lin Meng Zhi Wei Dang Cheng Chao Shou Beijing Institute for Cancer Research,Oncology School of Beijing Medical University,Beijing 100034,China Institute of Cancer Research,Chinese Academy of Medical Sciences,Beijing 100021,China | 2000 | World Journal of Gastroenterology2000，6，2： | 17 |
| 3 | 内镜下三线减张法与鼻中隔黏膜下切除术矫正鼻中隔偏曲疗效分析显示文摘三线减张法鼻中隔矫正术为2009年3月韩德民等[1]根据鼻中隔偏曲产生的生物力学原理,通过解除核心区域的张力,形成新的张力纠正鼻中隔偏曲,即三线减张法。笔者所在科室于2011年4月~2013年10月行内镜下三线减张法鼻中隔矫正术70例, | 张春雨 | 2013 | 中国耳鼻咽喉头颈外科2013，20，12： | 17 |
| 4 | Co-transfection of MRP and bcl-2 antisense S-oligodeoxynucleotides reduces drug resistance in cisplatin-resistant lung cancer cells显示文摘Obejctive To detect the influence of antisense s oligodeoxynucleotides (S ODNs) of bd 2 and multidrug resistamce associated protein (MRP) genes multidrug resistance associated protein gene and bcl 2 antisense S oligodeoxynucleotides on cisplatin resistant lung adenocarcinoma cell line A 549 DDP which overexpresses both bcl 2 and MRP Methods A 549 DDP cells were treated with sense and antisense S ODN mediated by lipofection Expression of MRP and bcl 2 mRNA and protein in the treated cells was measured by RT PCR and flow cytometry (FCM), respectively Apoptosis was identified by DNA electrophoresis and terminal deoxynucleotidyl transferase (TdT) mediated biotin dUTP nick end labeling(TUNEL) The degree of drug resistance of the treated cells was detected by a cell viability 3’ [4,5 dimethylthiazol 2 yl] 2,5 diphenyl tefrazolium bromide thiazolylblue (MTT) assay Results Expression of bcl 2 and MRP significantly decreased in the cells treated with bcl 2 or/and MRP antisense S ODN for 48h as compared to the cells untreated and sense treated ( P <0 05) Resistance to cisplatin in the cells treated with bcl 2 or/and MRP antisense S ODN decreased by 60 6% (6 5 times), 56 4% (7 2 times) and 71 0% (4 8 times), respectively, which paralleled the decrease of bcl 2 and MRP expression Similarly, the resistance to etoposide and epirubicin in antisense treated cells also reduced in parallel to decreases of the two gene expressions The drug resistance in sense treated cells was similar to that in untreated cells Statistically significant dose and concentration dependent increases of apoptotic cells were observed in the groups exposed to 100?μmol/L cisplatin for 48?h after treatment by bcl 2 or/and MRP antisense Conclusion Bcl 2 and MRP were at least additive and possibly synergistic in conferring drug resistance in a cisplatin resistant lung adenocarcinoma cell line Antisense S ODN could attenuate drug resistance by promoting cells apoptosis, which might lead to a new treatment for patients with non small cell lung cancers (NSCLCs) who are refractory to conventional | 王洁 刘叙仪 蒋薇 | 2000 | Chinese Medical Journal2000，，10： | 16 |
| 5 | Effect of cell fusion on metastatic ability of mouse hepatocarcinoma cell lines显示文摘INTRODUCTIONItwasknownduringthe1970sthatthemalignancyofhybridomacelsdecreasedwhentumorcelswerefusedwithnormalcelsandthemalign... | JI Yan 1, LING Mao Ying 1, LI Ying 1 and XIE Hong 2 | 1999 | World Journal of Gastroenterology1999，5，1： | 12 |
| 6 | Development of Chinese Near-isogenic Line of Rice and Their Differentiating Ability of Pathogenic Races of Blast Fungus显示文摘The rice( Oryza L. ) variety, Lijiangxintuanheigu(LTH), is a universally susceptible variety. A set of near-isogenic lines (NILs) of rice which has single resistance gene for each line was developed by use of LTH as recurrent parent and Kusabue,Tsuyuake, K1, PiNo. 4, K60 and BL1, which are from Kiyosawa's differential varieties(KDV), as donor parents. Differentiating ability of these NILs to Philippine isolates was compared with that of their donor parents, KDV and IRRI' s NILs. The results of comparative studies were as follows: (1) Differentiating ability of Chinese NILs to Philippine isolates was much stronger than that of their donor parents and that of KDV. (2) Chinese NILs had similar ability to differentiate races to IRRI's NILs or higher differentiating ability than them. But IRRI's NILs almost had no differentiating ability to isolates from japonica rice-growing regions. They can not be used to differentiate races of isolates from japonica ricegrowing regions. Whereas, Chinese NILs can be used to differentiate races of isolates from both japonica ricegrowing regions and indica rice-growing regions. It was concluded that the set of Chinese NILs can be used as international differentials. | LING Zhong-zhuan T. Mew WANG Jiu-lin LEI Cai-ling HUANG Ning | 2001 | Agricultural Sciences in China2001，，1： | 11 |
| 7 | Apoptosis of neoplasm cell lines induced byhepatic peptides extracted from sucking porcine hepatocytes显示文摘 | Kong XP Zou QY Li RB Zheng PL Yang LP Jin SW | 1999 | World Journal of Gastroenterology1999，5，5： | 11 |
| 8 | 鼻内镜下三线减张鼻中隔矫正手术显示文摘鼻中隔偏曲影响患者鼻腔通气及引流功能,导致一系列临床症状,其发生率在16%~79%。传统的治疗方法有鼻中隔黏膜下矫正术和鼻中隔黏膜下切除术,易引起一些并发症,如鼻中隔穿孔、鼻中隔摆动、鼻外形改变等。2009年韩德民等根据鼻中隔偏曲产生的生物力学原理,设计三线减张鼻中隔矫正法,我们从2009年11月~2011年8月,参照三线减张鼻中隔矫正手术方法进行手术,疗效满意。 | 孙西峰 庞晓明 | 2011 | 中国耳鼻咽喉头颈外科2011，18，11： | 10 |
| 9 | Characterization and evaluation of rice blast resistance of Chinese indica hybrid rice parental lines显示文摘The development of resistant varieties and hybrid combinations has been the most effective and economical strategy to control blast disease caused by Magnaporthe oryzae.However, the distribution of major R genes and blast resistance characterization in hybrid rice parents has not been well investigated, resulting in their limited use in hybrid rice blast-resistance breeding. In the present study, 88 elite indica hybrid rice parental lines were evaluated with 30 isolates of M. oryzae collected from the main planting area of indica hybrid rice in China and were characterized for the presence of 11 major resistance genes using molecular markers. The pathogenicity assays showed that four types of hybrid rice parent line showed some resistance to M. oryzae. However, the proportions of highly resistant lines and the mean resistance frequency(RF) varied among the four types, with resistance in decreasing order shown by three-line restorer lines, three-line maintainer lines, two-line sterile lines, and two-line restorer lines. All 88 hybrid rice parental lines carried more than one R gene, but none carried the R genes Pi1 and Pi2. Although Pid3 and Pi9 were present only in three-line restorer lines and Pigm only in three-line maintainer lines, the remaining six R genes(Pib, Pid2, Pi5, Pia, Pi54, and Pita) were present in the four types of hybrid rice parent with significantly different distribution frequencies. The correlation between R genes and resistance reactions was investigated. The results are expected to provide useful information for rational utilization of major R genes in hybrid rice breeding programs. | Yunyu Wu Ling Yu Ning Xiao Zhengyuan Dai Yuhong Li Cunhong Pan Xiaoxiang Zhang Guangqing Liu Aihong Li | 2017 | The Crop Journal2017，5，6： | 10 |
| 10 | Review on the applications and developments of drag reducing polymer in turbulent pipe flow显示文摘Drag reduction phenomenon in pipelines has received lots of attention during the past decades due to its potential engineering applications, especially in fluid transporting industries. Various methods to enhance drag reduction have been developed throughout the years and divided into two categories;non-additives method and additives method. Both categories have different types of methods, with different formulations and applications which will generally be discussed in this review. Among all the methods discussed, drag reduction using polymer additive is as one of the most enticing and desirable methods. It has been the subject of research in this field and has been studied extensively for quite some time. It is due to its ability to reduce drag up to 80% when added in minute concentrations. Reducing drag in the pipe will require less pumping power thus offering economic relieves to the industries. So, this paper will be focusing more on the use of polymer additives as drag reducing agent, the general formulations of the additives, major issues involving the use of drag reducing polymers, and the potential applications of it. However, despite the extensive works of drag reduction polymer, there are still no models that accurately explain the mechanism of drag reduction. More studies needed to be done to have a better understanding of the phenomenon. Therefore, future research areas and potential approaches are proposed for future work. | M.A.Asidin E.Suali T.Jusnukin F.A.Lahin | 2019 | Chinese Journal of Chemical Engineering2019，27，8： | 10 |
| 11 | Comparative study on CO_2 corrosion behavior of N80,P110,X52 and 13Cr pipe lines in simulated stratum water显示文摘To better understand the carbon dioxide (CO2) corrosion behavior of carbon steel and its influence on petroleum development (including drilling,production and transportation) in the Daqing Oilfield,CO2 corrosion behaviors of N80,P110,X52 and 13Cr pipe lines in simulated solution at high temperature and high pressure condition are investigated by dynamic corrosion experiments,scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS) analyses.Results show that the corrosion rate of all pipe lines increases quickly when CO2 partial pressure increases from 0.5 to 1.5 MPa,but it slows down when CO2 partial pressure further increases from 1.5 to 4.5 MPa,during which localized corrosion is prevailing.When the experimental temperature ranges from 60 to 120°C,localized corrosion prevails.The corrosion rate of all pipe lines decreases sharply when the temperature changes from 60 to 100°C,and it becomes stable after the temperature is higher than 100°C.When the flow rate is in the range of 0–1.5 m/s,the corrosion rate of sample X52 remains unchanged,but localized corrosion gradually becomes very serious when the flow rate further increases.By analyzing the corrosion product scales of sample 13Cr,some remarkable phenomena are observed.At the temperature range of 60–100°C,the corrosion product scales are loose and thick,but become very compact when the temperature increases above 100°C.In the later case,localized corrosion is prevailing.The main components of the corrosion product scales are FeCO3 and Cr2O3. | CAI YiDong1,GUO PengChao1,LIU DaMeng1,CHEN ShiYu2 & LIU JunLai3 1 School of Energy Resources,China University of Geosciences,Beijing 100083,China 2 School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing 100083,China 3 School of Earth Sciences and Resources,China University of Geosciences,Beijing 100083,China | 2010 | Science China(Technological Sciences)2010，53，9： | 10 |
| 12 | KAI1 inhibits HGF-induced invasion of pancreatic cancer by sphingosine kinase activity显示文摘BACKGROUND:KAI1/CD82 has been reported to attenuate the process of metastases in a variety of tumors;however,its mechanism of action in invasion has not been fully elucidated. The present study aimed to investigate the importance of KAI1 in invasion and its correlation with activation of sphingosine kinase(SPK)in human pancreatic cancer PANC1 and Miapaca-2 cell lines. METHODS:The expression of KAI1 in PANC1 and Miapaca-2 cells,which was mediated by recombinant adenovirus(Ad-KAI1), was assessed by a flow cytometer and Western blotting.After successful infection was established,in vitro growth curve and invasive ability in Boyden Chamber assay were studied.The presence of KAI1 correlating with c-Met and SPK was detected by co-immunoprecipitation and[γ-32P]ATP incorporation. RESULTS:KAI1 genes had no significant effects on the curve representing cell growth.After infection with the KAI1 gene,decreased invasive ability in the Boyden Chamber assay was observed in PANC1 and Miapaca-2 cells that were induced by hepatocyte growth factor.Over-expression of KAI1 in the cells led to the deactivation of SPK and a decreased level of intracellular sphingosine-1-phosphate.No correlation was observed between c-Met and KAI1 during co-immunoprecipitation. CONCLUSION:The results of this study for the first time demonstrated a regulatory role for KAI1 in SPK activation, which leads to decreased invasive ability in disease progression of human pancreatic cancer. | Xu Liu,Xiao-Zhong Guo,Wei-Wei Zhang,Zhuo-Zhuang Lu,Qun-Wei Zhang, Hai-Feng Duan and Li-Sheng Wang State Key Laboratory of Cancer Biology and Institute of Digestive Diseases,Xijing Hospital of Digestive Disease,Fourth Military Medical University,Xi’an 710032,China Department of Gastroenterology,Shenyang General Hospital of PLA,Shenyang 110016, China Department of Experimental Hematology,Beijing Institute of Radiation Medicine,Beijing 100850,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，2： | 9 |
| 13 | Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7显示文摘Objective:To build GPC3 gene short hairpin interference RNA(shRNA)slow virus veclor.observe expression of Huh-7 GPC3 gene in human liver cell line proliferation apoptosis and the effect of GPC3 gene influencing on liver cancer cell growth,and provide theoretical basis for genc therapy of liver cancer.Methods:Hepatocellular carcinoma cell line Huh-7 wsa transfected by a RNA interference technique.GPC3 gene expression in a variety of liver cancer cell lines was detected by fluorescence quantitative PCR.Targeted GPC3 gene seqnences of small interfering RNA(siRNA)PGC-shRNA-GPC3 were restructured.Stable expression cell linse of siRNA were screened and established with the heplp of liposomes(lipofectamine^(TM2000))as carrier transfcetion of human liver cell lines.In order to validate siRNA interference efficiency.GPC3 siRNA mRNA expression was detected after transfection by using RT-PCR and Western blot.The absorbance value of the cells of blank group,untransfection group and transfection group,the cell cycle and cell apoptosis were calculated,and effects of GPC3 gene nn Huh-7 cell proliferation and apoptosis were observed.Results:In the liver cancer cell lines Huh-7 GPC3 gene showed high expression.PGC-shRNA-GPC3 recombinant plasmid was constructde successfully via sequencing validation.Stable recombinant plasmid transfected into liver cancer cell linse Huh-7can obviously inhibit GPC3 mRNA expression level.Conclusions:The targeted GPC3 siRNA can effectively inhibit the expression of GPC3. | Chang-Jiang Lei Chun Yao Qing-Yun Pan Hao-Cheng Long Lei Li Shu-Ping Zheng Cheng Zeng Jian-Bin Huang | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，10： | 8 |
| 14 | Cloning of UGT1A9 cDNA from liver tissues and its expression in CHL cells显示文摘AIM: To clone the cDNA of UGT1 A9 from a Chinese human liver and establish the Chinese hamster lung (CHL) cell line expressing human UGTI A9.METHODS: cDNA of UGT1A9 was transcripted from mRNA by reverse transcriptase-ploymerase chain reaction, and was cloned into the pGEM-T vector which was amplified in the host bacteric E. CoIl DH5α. The inserted fragment, verified by DNA sequencing, wes subcloned into the Hind III/Not I site of a mammalian expression vector pREP9 to construct the plasmid termed pREP9-UGT1A9. CHL cells were transfected with the resultant recombinants, pREP9- UGT1A9, and selected by G418 (400 mg. L-1) for one month. The surviving clone (CHL-UGT1A9) was harvested as a pool and sub-cultured in medium containing G418 to obtain samples for UGT1 A9 assays. The enzyme activity of CHL-UGT1A9 towards propranolol in S9 protein of the cell was determined by HPLC.RESULTS: The sequence of the cDNA segment cloned,which was 1666 bp in length, was identical to that released by Gene Bank (GenBank accession number: AF056188) in coding region. The recombinant constructed, pREP9- UGT1 A9, contains the entire coding region, along with 18bp of the 5' and 55 bp of the 3′ untranslated region of the UGT1A9 cDNA, respectively. The cell lines established expressed the protein of UGT1 A9, and the enzyme activity towards propranolol in S9 protein was found to be 101 ± 24pmol.min-1 .mg-1 protein (n = 3), but was not detectable in parental CHL cells.CONCLUSION: The cDNA of UGT1A9 was successfully cloned from a Chinese human liver and transfected into CHL ceils. The CHL-UGT1A9 cell lines established efficiently expressed the protein of UGT1A9 for the further enzyme study of drug glucuronidation. | Xin Li~(1,2) Ying-Nian Yu~1 Ge-Jian Zhu~1 Yu-Li Qian~1 1 Department of Pathophysiology,School of Medicine,Zhejiang University,Hangzhou,China2 College of Pharmcy,Zhejiang University,Hangzhou,China | 2001 | World Journal of Gastroenterology2001，7，6： | 8 |
| 15 | 架空输电线路微风振动数据分析与工程应用研究显示文摘针对宁夏沿山输电线路架空地线多年频繁断股问题,引进了微风振动在线监测系统,对330 kV大铜Ⅰ回线#12塔和220 kV大青丙线#16塔导、地线进行振动监测,并与输电线路振动判定标准进行对比,发现地线中频区段动弯应变值超标,采取计算、模拟试验等方法,设计并实施了两种改进的防振方案,明显降低了地线振动水平,为输电线路微风振动治理及工程应用研究提供了参考依据。 | 王波 毛吉贵 方俊杰 徐天山 | 2010 | 水电能源科学2010，28，10： | 7 |
| 16 | 中国南瓜自交系的聚类分析(英文)显示文摘A cluster analysis was carried out based on Euclidean genetic distances through UPGMA method in Chinese pumpkin inbred lines.7 important agronomic traits of 46 Chinese pumpkin inbred lines were investigated.The result indicated that 46 pumpkin inbred lines were clustered into 4 groups and the inter-groups distances was larger than that in intra-group.The genetic distances of parents were related to F1 performance and the results of cluster would increase effectiveness in the Chinese pumpkin crossing breeding. | 杜晓华 李小梅 李新峥 | 2008 | Agricultural Science & Technology2008，9，4： | 7 |
| 17 | 植物基因组中的非LTR反转录转座子SINEs和LINEs显示文摘反转录转座子是基因组进化的推动者之一,分为LTR和非LTR两种类型。前者是真核基因组的主要组分,结构和转座方式与逆转录病毒类似。后者是最初发现于动物基因组新近发现在植物基因组中也广泛存在的新型重复序列,包括LINEs(long interspersed nuclear elements)和SINEs(short interspersed nuclear elements)两个亚型。它们大多因自身或受宿主基因组的调控而失去转座活性。其转座机理目前还不十分清楚,推测LINEs可以自主转座,SINEs依赖其他转座子被动转座。种系分析认为LINEs可能是最古老的反转录转座子,SINEs的起源未知。文章对以上内容进行了归纳和讨论。 | 程旭东 凌宏清 | 2006 | 遗传2006，28，6： | 7 |
| 18 | 基于Color Lines先验的高阶马尔科夫随机场去雾显示文摘传统的一阶马尔科夫随机场在图像先验信息表达和对图像整体的约束上能力有限,同时基于暗通道的去雾算法在天空等大片白色区域处理效果存在偏差。针对以上问题,该文提出一种基于Color Lines的高阶马尔科夫随机场去雾算法。该算法通过引入对颜色失真具有很好鲁棒性的Color Lines先验条件,初步校正经暗通道获取的传输图,然后利用高阶马尔科夫随机场优化传输图,获取最终精确的去雾图像。实验结果表明,与已有算法相比,该文算法具有更强的普适性,可提高雾天图像的清晰度,同时恢复更多的图像细节等信息。 | 毕笃彦 眭萍 何林远 马时平 | 2016 | 电子与信息学报2016，38，9： | 6 |
| 19 | RETROVIRAL MEDIATED EFFICIENT TRANSFER ANDEXPRESSION OF MULTIPLE DRUG RESISTANCE GENE TO HUMAN LEUKEMIC CELLS显示文摘Objective: To investigate retroviral-mediated transfer and expression of human multidrug resistance (MDR) gene MDRI in leukemic cells. Methods: Human myeloid cells, K562 and NB4, were infected by MDR retrovirus from the producer PA317/HaMDR, and the resistant cells were selected with cytotoxic drug. The transfer and expression of MDRI gene was analyzed by using polymerasc chain reaction (PeR), now cytometry (FCM) and scmisolid colonies cultivation. Results: The resistant cells, K562/MDR and NB4/MDR, in which integration of the exogenous MDRI gene was confirmed by PCR analysis, displayed a typical MDR phenotype. The expression of MDR1 transgenc was detected on truncated as well as full-length transcripts. Moreover, the resistant cells were P-glycoprotein positive at 78.0% to 98.7% analyzed with FCM. The transduction efficieny in K562 cells was studied on suspension cultures and single-cell colonies. The transduction was more efficient in coculture system (67.9%-72.5%) than in supernatant system (33.1%-46.8%), while growth factors may improve the efficiency. Conclusion: Retrovirus could allow a functional transfer and expression of MDR1 gene in human leukemia cells, and MDRI might act as a dominant selcctable gene for coexpression with the genes of interest in gene therapy. | 傅建新 王玮 岑建军 李建勇 阮长耿 陈子兴 | 2000 | Chinese Journal of Cancer Research2000，12，2： | 6 |
| 20 | 基于ICP和CPD的颅骨自动配准算法显示文摘颅骨配准是计算机辅助的三维颅面复原技术的重要研究内容之一。颅骨配准的准确与否会直接影响到将来颅面复原的准确性。为此,提出一种新的3D颅骨自动配准算法。该算法考虑到颅骨模型的特殊结构与实现的简便性,首先自动提取颅骨不光滑区域的脊线(Crest lines)以及光滑区域的顶点作为特征点,然后利用迭代最近点(ICP)算法进行粗配准,在此基础上,再采用CPD(Coherent Point Drift)算法对颅骨进行精确配准。实验结果表明,该算法能有效提高颅骨配准的准确性并对缺损颅骨具有一定的鲁棒性。 | 白茹意 周明全 邓擎琼 | 2011 | 计算机技术与发展2011，21，2： | 6 |
您所在的机构暂未开通此部分的功能，请联系管理员