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| 1 | 乳酸菌发酵液体饲料对生长猪生长性能和粪中微生物区系的影响显示文摘发酵液体饲料(Fermented liquid feed,FLF)作为一种新型饲料,最早使用是在20世纪80年代末的荷兰,当时的发酵液体饲料实际上就是湿拌料(陈文斌,2004)。随着现代饲料工业技术的发展,发酵液体饲料正朝着可人工控制发酵过程的方向发展(王金全,2009)。FLF较传统干料和湿拌料(NFLF)有许多优点,如可使用食品发酵工业副产物,大大降低饲料成本; | 陈鲜鑫 王金全 王春阳 张广民 孙占敏 | 2010 | 饲料工业2010，31，4： | 35 |
| 2 | 多壁碳纳米管固相萃取净化-高效液相色谱法测定猪肉和鸡肉中的磺胺多残留显示文摘建立了多壁碳纳米管为吸附剂的固相萃取净化-高效液相色谱-紫外检测测定猪肉和鸡肉中多种磺胺类药物多残留的方法。样品采用乙腈提取,多壁碳纳米管固相萃取净化,NaH2PO4缓冲溶液(pH5.5~6.0)溶解上样,5%(v/v)丙酮-正己烷淋洗,丙酮-二氯甲烷(1:1,v/v)洗脱。色谱分离以50mmol/LNaH2PO4-乙腈(7:3,v/v)为流动相,方法的线性范围为0.01~1.00mg/L,线性相关系数大于0.998,检出限(LOD)为0.003mg/L,定量限(LOQ)为0.01mg/L。在0.02~0.2mg/kg添加范围内,9种磺胺类药物的回收率高于70%,RSD低于8%,表明多壁碳纳米管对磺胺类药物具有较强的吸附富集能力。该方法简便、准确可用于动物组织及产品中磺胺药物残留的检测。 | 赵海香 刘海萍 闫早婴 | 2014 | 色谱2014，32，3： | 40 |
| 3 | 超高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱快速筛查化妆品中的24种激素显示文摘建立了超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UPLC-LTQ/Orbitrap MS)快速筛查、定性识别化妆品中24种激素的分析方法。不同剂型的化妆品样品经甲醇超声提取,用Waters ACQUITY UPLC BEH C18色谱柱(50 mm×2.1 mm,1.7μm)分离,以乙腈和0.1%(v/v)甲酸水溶液为流动相进行梯度洗脱。通过静电场轨道阱全扫描得到激素化合物的准分子离子的精确质量数,实现对化妆品中激素的快速筛查;再以保留时间和数据依赖扫描(data dependent scan)模式获得的子离子质谱图进行定性确证。24种激素化合物的质量准确度误差小于3×10-6(3 ppm);线性良好,相关系数大于0.99;检出限≤10μg/kg(S/N=3),能满足实际化妆品样品的分析要求。应用该方法对不同剂型的50余种化妆品样品进行筛查分析,结果良好。该方法是化妆品中激素快速筛查、定性识别的有效方法。 | 李兆永 王凤美 牛增元 罗忻 张罡 陈军辉 | 2014 | 色谱2014，32，5： | 33 |
| 4 | Green chemistry of chromate cleaner production显示文摘Research topics and methods of green chemistry in chromate production are introduced in this paper . New original green chemical process of the heterogeneous reaction and separation system of liquid phase oxidation of chromite in molten salt of sodium hydroxide-one way separation in high concentration medium-metastable phase separation-carbonate recycle conversion has been developed. The green commercial process for comprehensive utilization of mineral resources-reactant recycle inside the process-zero emission was established . | 张懿 李佐虎 齐涛 王志宽 郑诗礼 | 1999 | Chinese Journal of Chemistry1999，17，3： | 27 |
| 5 | Progress of gastric cancer etiology:N-nitrosamides in the 1990s显示文摘INTRODUCTIONStomach carcinoma is still the leading cause of cancer deathin China and the second one in the world.Its possiblecauses include:A)chemical factors such as intragastricformation of N-nitroso compounds(NOC)and high saltintake;B)biological factors such as infection | Da Jun Deng Laboratory of Cancer Etiology,Beijing Medical University School of Oncology& Beijing Institute for Cancer Research,100034,China | 2000 | World Journal of Gastroenterology2000，6，4： | 27 |
| 6 | 加速溶剂萃取-高效液相色谱串联质谱法测定土壤中农药残留显示文摘1引言土壤中农药残留可分为杀虫剂、除草剂和杀菌剂残留。常见杀虫剂有克百威和甲氰菊酯等,常见除草剂有苯噻酰草胺、乙草胺、丙草胺、喹禾灵和草酮等,常见杀菌剂有烯酰吗啉和稻瘟灵等。克百威是广谱性杀虫剂,它与胆碱酯酶结合不可逆,因此毒性很高;乙草胺是使用量极大的除草剂之一。这两种农药的毒性远高于其它几种常见农药。快速的前处理及稳定的仪器检测是研究土壤中农药残留的关键,尤其是各类型农药的同时检测,不仅可以提高检测效率。 | 闫蕊 邵明媛 鞠福龙 宋大千 张寒琦 于爱民 | 2013 | 分析化学2013，41，2： | 26 |
| 7 | 固相萃取-超高效液相色谱-电喷雾串联质谱法同时测定地表水中16种全氟有机化合物显示文摘建立了固相萃取-超高效液相色谱-电喷雾串联三重四极杆质谱联用技术分析水中16种全氟有机化合物的高通量检测方法。样品经 WAX 固相萃取柱富集和净化后,采用 Waters ACQUITY UPLCTM BEH C18色谱柱,含2 mmol/L乙酸铵的甲醇和含2 mmol/L乙酸铵的水作为流动相进行梯度洗脱,质谱采用电喷雾负离子电离,多反应监测模式检测。16种全氟有机化合物在0.5~100μg/L或1.0~100μg/L 浓度范围内线性关系良好,相关系数为0.9987~0.9999,方法的检出限为0.06~0.46 ng/L;高、中、低3个添加水平的回收率为67.6%~103%,相对标准偏差为2.94%~12.0%。结果表明,该方法灵敏、准确且检测范围广,分析速度快,是一种适于实际水样中全氟有机化合物检测分析的方法。 | 张明 唐访良 俞雅雲 陈峰 徐建芬 叶永根 | 2014 | 色谱2014，32，5： | 22 |
| 8 | 快速高效液相色谱法测定卷烟主流烟气中的酚显示文摘 | 陈章玉 张承明 徐若飞 孔维松 杨光宇 苏庆德 | 2006 | 色谱2006，24，2： | 20 |
| 9 | Imperatorin Is Responsible for the Vasodilatation Activity of Angelica Dahurica var. Formosana Regulated by Nitric Oxide in an Endothelium-Dependent Manner显示文摘Objective:To demonstrate the vasodilatation activity of the coumarin-containing Angelica dahurica var.formosana and to further analyze active components in the herb extracts.Methods:(1) The vasodilatation effects induced by different extracts(cyclohexane,ethyl acetate,acetone,methanol,95%ethanol and water) of Angelica dahurica var.formosana on mouse thoracic aorta pre-contracted with phenylephrine were investigated. (2) The amount of imperatorin and isoimperatorin in each extract was measured by high-per... | 聂红 孟兰贞 周玖瑶 樊晓峰 罗勇 张广文 | 2009 | Chinese Journal of Integrative Medicine2009，15，6： | 17 |
| 10 | 基于超高效液相色谱-质谱分析的代谢组学在小檗碱治疗多囊卵巢综合征研究中的应用显示文摘多囊卵巢综合征(polycystic ovary syndrome,PCOS)是妇女最常见的内分泌和代谢紊乱性疾病,合理的药物治疗非常重要。本研究中,超重/肥胖的多囊卵巢综合征患者经过小檗碱治疗3个月,采集治疗前后的血样进行分析,利用超高效液相色谱-质谱联用技术(UHPLC-MS)对治疗前后血清中的内源性物质进行了相对含量测定。正交信号校正的偏最小二乘模式识别分析结果显示治疗前后有明显的区分。多变量分析结合非参数检验找出的与小檗碱治疗相关的差异代谢物均与脂类代谢有关。临床生化数据结合代谢组学数据显示,小檗碱治疗超重/肥胖型的多囊卵巢综合征患者可以增加机体胰岛素敏感性、改善脂类代谢。上述研究结果表明代谢组学是研究中药治疗疾病效果的有效工具之一。 | 李艳杰 张春兰 张晗 赵欣捷 侯丽辉 许国旺 | 2014 | 色谱2014，32，5： | 17 |
| 11 | On Mechanism of Heat-Conduction and Equation of Thermal Conductivity of Liquid显示文摘The recently presented equation for nonequilibrium state of gases is hereextended to liquid,and thus an equation of thermal conductivity for normal liquid is de-rived.The equation is applied to both polar and nonpolar liquids,with the average error of608 experlmental values being 1.75% based upon 110 compounds of 29 types of structures.This method is far better than all the other methods published. | 张克武 | 1993 | Science China Mathematics1993，36，3： | 16 |
| 12 | 超高效液相色谱-串联质谱法测定豇豆中溴氰虫酰胺等5种农药残留显示文摘建立了超高效液相色谱-串联质谱( UPLC-MS/MS)同时测定豇豆中溴氰虫酰胺、乙基多杀菌素、呋虫胺、噻虫嗪和啶虫脒残留量的方法。豇豆样品经乙腈提取,通过QuEChERS法净化后,采用Acquity UPLC?? BEH C18色谱柱分离,在电喷雾正离子模式下以多反应监测( MRM)模式检测。结果表明:在0.01~1μg/mL内5种农药的峰面积与其相应的质量浓度间呈良好线性关系,相关系数均大于0.99;在0.01、0.1和1 mg/kg 3个添加水平下,回收率为71%~129%,相对标准偏差为0.90%~20.8%( n =5)。5种农药在豇豆中的检出限为0.084~4.0μg/kg,定量限为0.80~16.6μg/kg。该方法简单灵敏,定量准确,可用于分析豇豆中的溴氰虫酰胺、乙基多杀菌素、呋虫胺、噻虫嗪和啶虫脒的残留量。 | 钱程 吴琼 吕岱竹 李建国 | 2014 | 农药学学报2014，16，5： | 16 |
| 13 | 固相萃取-高效液相色谱法测定鱼组织中的吡喹酮和克螨特残留显示文摘 | 吴晓苹 王玲 施点望 陈天文 谢增鸿 阎超 | 2005 | 色谱2005，23，4： | 15 |
| 14 | 柱前衍生高效液相色谱法同时测定水性涂料中6种醛类化合物显示文摘建立了柱前衍生高效液相色谱同时测定水性涂料中甲醛、乙醛、丙醛、苯甲醛、正戊醛和对甲基苯甲醛等6种醛类化合物的方法。样品经水超声提取后,与2,4-二硝基苯肼乙腈溶液在酸性条件下衍生,再经0.45μm 针式过滤器过滤后进样分析。系统考察了酸度调节剂、pH 值、反应温度、时间等因素对衍生反应的影响,优化的反应条件为:以稀释后的盐酸溶液作为酸度调节剂,缓冲溶液 pH =3,反应温度为60℃,反应时间为30 min。在此条件下,于0.08~2.0 mg / L 浓度范围内,6种醛类化合物呈良好的线性关系;检出限为0.05~2.50 mg / kg;在2.0、4.0、6.0 mg / kg 3个水平下的加标回收率为87.0%~112.8%;RSD 为1.12%~9.54%。结果表明,本方法具有较宽的线性范围,良好的精密度和准确度,适用于水性涂料中6种醛类化合物的同时测定。 | 陈潜 周宇艳 程欲晓 林苗 | 2014 | 色谱2014，32，3： | 14 |
| 15 | Cell-free circulating tumor DNA in cancer显示文摘Cancer is a common cause of death worldwide.Despite significant advances in cancer treatments,the morbidity and mortality are still enormous.Tumor heterogeneity,especially intratumoral heterogeneity,is a significant reason underlying difficulties in tumor treatment and failure of a number of current therapeutic modalities,even of molecularly targeted therapies.The development of a virtually noninvasive 'liquid biopsy' from the blood has been attempted to characterize tumor heterogeneity.This review focuses on cell-free circulating tumor DNA(ctDNA) in the bloodstream as a versatile biomarker.ctDNA analysis is an evolving field with many new methods being developed and optimized to be able to successfully extract and analyze ctDNA,which has vast clinical applications.ctDNA has the potential to accurately genotype the tumor and identify personalized genetic and epigenetic alterations of the entire tumor.In addition,ctDNA has the potential to accurately monitor tumor burden and treatment response,while also being able to monitor minimal residual disease,reducing the need for harmful adjuvant chemotherapy and allowing more rapid detection of relapse.There are still many challenges that need to be overcome prior to this biomarker getting wide adoption in the clinical world,including optimization,standardization,and large multicenter trials. | Zhen Qin Vladimir A.Ljubimov Cuiqi Zhou Yunguang Tong Jimin Liang | 2016 | Chinese Journal of Cancer2016，35，5： | 14 |
| 16 | 固相萃取-超快速液相色谱-串联质谱法同时测定血液中的4种苏丹染料显示文摘建立了血液样品中4种苏丹染料(苏丹Ⅰ、Ⅱ、Ⅲ和Ⅳ)的固相萃取-超快速液相色谱-串联质谱( UFLC-MS /MS)测定方法。样品经乙腈涡旋振荡提取,上清液加等体积水稀释混匀后移入 C18固相萃取小柱净化,采用 Agi-lent Eclipse Plus C18色谱柱(100 mm×2.1 mm,1.8μm)以0.1%(v / v)甲酸水溶液和0.1%(v / v)甲酸乙腈溶液为流动相梯度洗脱分离,电喷雾电离(ESI)正离子多反应监测模式进行定量分析。讨论了苏丹Ⅲ和苏丹Ⅳ的偶氮基团 E-Z 光学异构现象,并对影响因素进行了分析。结果4种苏丹染料在0.1~20.0μg / L 范围内线性关系良好,相关系数均大于0.999;在低、中、高3个加标水平的平均回收率为93.0%~108.2%,相对标准偏差为4.8%~9.5%;方法的检出限(LOD)为0.06μg / L,定量限(LOQ)为0.2μg / L。本方法准确、快速、灵敏,可用于血液样品中苏丹类染料的检测分析。 | 朱浩 黄坤玉 付建飞 胡岳 黄娴妮 陈晓红 邹宝波 金米聪 | 2014 | 色谱2014，32，3： | 13 |
| 17 | 超高效液相色谱-三重四极杆串联质谱法测定柑橘中4种苯甲酰脲类农药残留显示文摘建立了柑橘中虱螨脲、灭幼脲、氟啶脲和除虫脲4种苯甲酰脲类农药同时检测的超高效液相色谱-三重四级杆串联质谱( UPLC-MS/MS)分析方法。样品采用QuEChERS前处理技术,超高效液相色谱-串联质谱电喷雾负离子模式检测测定。结果表明:在0.01~0.2μg/mL范围内,4种供试农药的质量浓度与其相应的峰面积间呈良好的线性关系,相关系数( r )均大于0.994。在0.01、0.05和0.1 mg/kg 3个添加水平下,4种农药的平均回收率为92%~105%,相对标准偏差(RSD)为0.4%~3.3%(n=5)。4种农药在柑橘中的定量限(LOQ)均为0.01 mg/kg。该方法准确、灵敏、简单,适用于同时测定柑橘中4种苯甲酰脲类农药的残留量。 | 张月 林靖凌 韩丙军 吕岱竹 | 2014 | 农药学学报2014，16，5： | 12 |
| 18 | 药用真菌粗毛纤孔菌的分子甄别及其发酵液抗乳腺癌活性研究显示文摘从分子水平上探究粗毛纤孔菌与桑黄孔菌属菌株的差异,同时以人乳腺癌MDA-MB-231细胞筛选粗毛纤孔菌深层发酵液抗乳腺癌活性部位,并对其成分进行初步分析。基于r DNA ITS序列分子系统发育树和拆分网络(splits network)分析研究粗毛纤孔菌与桑黄孔菌属菌株的差异;采用MTT法检测发酵液不同提取物抗乳腺癌活性;通过细胞形态观察进一步了解活性提取物对细胞生长的影响;采用LC-MS/MS对活性提取物的成分进行分析。系统发育树表明粗毛纤孔菌与桑黄孔菌属菌株存在一定的遗传差异,粗毛纤孔菌GC含量较桑黄孔菌属菌株低,同时拆分网络分析法能更好地区分粗毛纤孔菌与桑黄;粗毛纤孔菌深层发酵液正丁醇提取物对MDA-MB-231细胞生长的抑制作用最强且呈量效和时效关系(P<0.05),作用24h的IC50值为245.44μg/mL;细胞形态观察显示正丁醇提取物浓度在250μg/mL时,对MDA-MB-231细胞的凋亡作用随着时间的延长而加强;LC-MS/MS检测到正丁醇萃取部分的物质有2个,这2个物质均首次在粗毛纤孔菌中鉴定得到。结果表明,拆分网络分析法可较好地用于粗毛纤孔菌与桑黄孔菌属菌株的分子甄别,正丁醇提取物是粗毛纤孔菌深层发酵液抗乳腺癌MDA-MB-231细胞的主要活性部位,为进一步开发利用粗毛纤孔菌药用资源提供科学依据。 | 刘鑫 侯若琳 金珊珊 戚梦 胡开辉 郑明锋 傅俊生 | 2018 | 菌物学报2018，37，2： | 12 |
| 19 | Study on the Relationship between Plasma Homocysteine and Acute Cerebral Vascular Disease显示文摘The levels of plasma homocysteine were determined by using high-performance liquid chromatographic method. It was found that plasma homocysteine levels were significantly higher in the patients with stroke than that in the controls. There was no correlation between plasma homocys- teine levels and hypertension, smoking, concentrations of blood glucose or hypertriglyceridesemia. It was suggested that hyperhomocysteinemia may be an independent risk factor for acute cerebral vascu- lar disease. | 彭海 黄谦 李月芬 孙圣刚 邓学军 乔娴 | 2000 | Journal of Huazhong University of Science and Technology(Medical Sciences)2000，20，4： | 11 |
| 20 | Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy显示文摘AIM:To perform plasma free amino acid(PFAA)profiling of esophageal squamous cell carcinoma(ESCC)patients at different pathological stages and healthy subjects.METHODS:Plasma samples from ESCC patients(n=51)and healthy control adults(n=60)were analyzed by high-performance liquid chromatography(HPLC).The ESCC patients included moderate/poorly-differentiation(n=24),lymph node metastasis(n=17)and clinical stage>Ib2(n=36).Partial least squares discriminant analysis was performed to demonstrate that the PFAA metabolic patterns enabled discrimination between ESCC patients and controls,and the Student t test was applied to assess significant differences in PFAA concentrations between the two groups.RESULTS:There were significant differences in the PFAA profiles between controls and ESCC patients.Compared with healthy controls,the levels of Asp,Glu,Gly,His,Thr,Tau,Ala,Met,Ile,Leu,and Phe were decreased in ESCC patients,but Cys was increased.There exists a strong correlation between PFAA profiles and clinicopathological characteristics in ESCC patients.The levels of many PFAAs(i.e.,Glu,Asp,Ser,Gly,Tau,Ala,Tyr,Val,Ile,and Leu)were related to pathological grading,lymph node metastasis,and ESCC clinical stage.Very good discrimination between ESCC patients and control subjects was achieved by multivariate modeling of plasma profiles.CONCLUSION:HPLC-based plasma profiling analysis was shown to be an effective approach to differentiate between ESCC patients and controls.PFAA profiles may have potential value for screening or diagnosing ESCC. | Hong Ma Ayshamgul Hasim Batur Mamtimin Bin Kong Hai-Ping Zhang Ilyar Sheyhidin | 2014 | World Journal of Gastroenterology2014，20，26： | 11 |
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