SF/HGF-c-Met autocrine and paracrine promote metastasis of hepatocellular carcinoma

查看全文 作　　者：Qian Xie Kang-Da Liu Mei-Yu Hu Kang Zhou Experimental Research Center of Zhongshan Hospital,Fudan University,Shanghai,200032,China

出　　处：《World Journal of Gastroenterology》索引2001年第7卷第6期,共5页

基　　金：Supported by Natural Science Foundation of China No.39970290 摘　　要：AIM: To explore the role of SF/HGF-Met autocrine and parscrine in metastasis of hepatocellular carcinoma (HCC).METHODS: SF/HGF and c-met transcription and protein expression in HCC were examined by RT-PCR and Western Blot in 4 HCC cell lines, including HepG2, Hep3B,SMMC7721 and MHCC-1, the last cell line had a higher potential of metastasis. Sf/hgf cDNA was transfected by the method of Lipofectin into SMMC7721. SF/HGF and c-met antibody were used to stimulate and block SF/HGF-c-met signal transduction. Cell morphology, mobility, and proliferation were respectively compared by microscopic observation, wound healing assay and cell growth curve.RESULTS: HCC malignancy appeared to be relative to its met-SF/HGF expression. In MHCC-1, c-met expression was much stronger than that in other cell lines with lower potential of metastasis and only SF/HGF autocrine existed in MHCC-1. After sf/hgf cDNA transfection or conditioned medium of MHCC-1 stimulation, SMMC7721 changed into elongated morphology, and the abilities of proliferation ( P < 0.05) and mobility increased. Such bio-activity could he blocked by c-met antibody ( P< 0.05).CONCLUSION: The system of SF/HGF-c-met autocrine and paracrine played an important role in development and metastasis potential of HCC. Inhibition of SF/HGF-c-met signal transduction system may reduce the growth and metastasis of HCC. 关键词：HEPATOCYTE growth factor/Scatter factor C-MET hepatocel1ular carcinoma METASTASIS

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SF/HGF-c-Met autocrine and paracrine promote metastasis of hepatocellular carcinoma

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