Effect of Danzhi decoction on expression of angiogenesis factors in patients with sequelae of pelvic inflammatory disease

查看全文 作　　者：Bao-Qin [1]Liu;Xin [2]Gong;Zhe [1]Jin

机构地区：[1]Beijing University of Chinese Medicine;[2]Department of Gynecology,Oriental Hospital of Beijing University of Chinese Medicine

出　　处：《Asian Pacific Journal of Tropical Medicine》索引2014年第7卷第12期,共6页

基　　金：supported by a grant from the National Natural Science Founlation of China(81373677) 摘　　要：Objective:To investigate the efteets of traditional Chinese medicine.Danzhi decoction,on the expression angiogenesis factors in human endometrial cells during the sequelae of pelvic inflammatoty disease(SPID) and explore the role of Danzhi decction in improving the blood stasis microenvironment of SPID.Methods:A three-dimensional(3D) co—culture system including human vascular endothelial cells(VECs),endometrial stromal cells and glandular epithelial cells was established in vitro and treated with Danzhi decoction,sterilized water and aspirin respectively.A Milliplex multifunctional liquid chip technique was used to measure the expression levels of vascular endothelial growth factor(VEGF)—A/C/D,fibroblast growth factor-1/2.angiopoietin-2.epidermal growth factor(EGF) HB-EGF,bone morphogenetic protein-9.endoglin.endothehn-l.granulocyte colony stimulating factor,hepatocyte growth factor,interleukin-8,follistatin.placenta growth factor and leptin.The location of angiogenesis factors was monitored by immunofluorescence labeling and confocal laser scanning microscope3 D reconstruction.Results:Endometrial stromal cells and glandular epithelial cells were isolated and primary cultured for establishing a 3D co-culture system.The levels of VEGF—A/C/D in Danzhi decoction group and aspirin group were significantly lower than those in mock group(P<0.05).while there was no significant difference between Danzhi decoction group and aspirin group(P>0.05).Furthermore,the alterative location of VEGF—A/C/D was observed in the cytoplasm of endometrial glandular epithelial cells.Conclusions:Danzhi decoction mav inhibit the expression of VEGF in the blood stasis microenvironment of SPID by targeting the cytoplasm of endometrial glandular epithelial cell. 关键词：Danzhi DECOCTION SEQUELAE of PELVIC inflammatory disease ENDOMETRIAL cells ANGIOGENESIS factor MICROENVIRONMENT

相关文献

参考文献（8）
引证文献（7）
耦合文献（41）

参考文献(8)

引证文献(7)

耦合文献(41)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Effect of Danzhi decoction on expression of angiogenesis factors in patients with sequelae of pelvic inflammatory disease

参考文献(8)

引证文献(7)

耦合文献(41)

用户提示