Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

查看全文 作　　者：Wei-Ping [1,2]Qian;Yue-Qiu [2]Tan;Ying [5]Chen;Ying [4]Peng;Zhi [3]Li;Guang-Xiu [2]Lu;Made C. [3]Liu;Hsiang-Fu [5]Kung;Ming-Ling [5]He;Li-Ka [5]Shing

机构地区：[1]Luofu Hospital, Shenzhen 518033, Guangdong Province, China;[2]Institute of Reproduction and Stem Cell Engineering, Xiangya School of Medicine, Central South University, Changsha 410008, Hunan Province, China;[3]The Institute of Molecular Biology, The University of Hong Kong, Hong Kong Special AdministrativeRegion, China;[4]Department of Neurology, The Second AffiliatedHospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China;[5]The Center for Emerging Infectious Diseases, The Chinese University ofHong Kong, Hong Kong Special Administrative Region, China

出　　处：《World Journal of Gastroenterology》索引2005年第11卷第34期,共5页

基　　金：Supported by Research Fund for the Control of Infectious Diseases and Research Grant Committee of Hong Kong Government 摘　　要：AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QTAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction.RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.L4×105 and 3.02×105 copies of HBV DNA per mL in the semen.CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. 关键词：精液检查乙型肝炎病毒病毒DNA 聚合酶链反应

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Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

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