Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

查看全文 作　　者：FAN XiaoJun ZHENG Bo FU YueJun SUN Yi LIANG [1]AiHua

机构地区：[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China

出　　处：《Chinese Science Bulletin》索引2008年第53卷第12期,共6页

基　　金：the National Natural Science Foundation of China (Grant Nos.30670282 and 30700534);the Department of Science and Technology of Shanxi Province 摘　　要：An Buthus martensii Karsch Insect Toxin (BmK IT ) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1×107 PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8h) during a 192h in-fection. 关键词：杀虫剂神经毒素生物测定基因组

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Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

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