Mining rice new germplasm containing S_5~n gene by functional molecular marker and sequencing

查看全文 作　　者：YANG YouXin WU JinWen CHEN ZhiXiong WANG Lan LI JinQuan LIU XiangDong LU [1]YongGen

机构地区：[1]Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China

出　　处：《Chinese Science Bulletin》索引2009年第54卷第18期,共7页

基　　金：Supported by Joint Fund of the National Natural Science Foundation of China-Guangdong Province (Grant No. U0631003) 摘　　要：Inter-subspecific hybrids between indica and japonica varieties yield strong biological heterosis, but it is difficult to utilize the hybrids directly in commercial production due to sterility of F1. A special gene S5n may overcome the hybrids sterility, which is caused by the interaction between S5 loci. Recently, S5n had been cloned, and it was revealed containing a large DNA deletion sequence that made the gene nonfunctional, compared to S5i or S5j. We designed a pair of primers flanking the deletion sequence of S5n, and then applied to distinguish the varieties with S5n or non-S5n, convincing result suggested that the primers could be served as functional molecular marker to efficiently identify the new germplasm with S5n. Using the functional marker, we surveyed 197 varieties from China National Micro-core Rice Collection, and found ten of which represented S5n including following varieties: Haobuka, Sanbangqishiluo, Mubanggu, Xiaohonggu, Mowanggu’neiza, Laozaogu, Fanhaopi, Feie’nuo2, Baoxie-7B, Teqingxuanhui. Among them, two varieties Sanbangqishiluo and Laozaogu was previously reported to contain S5n gene. Further sequence analysis on the DNA sequence covering both sides of deletion in S5n of the 10 varieties confirmed that the detected sequences in above varieties was identical with those of varieties containing S5n, such as 02428 and Linglun. These results suggested that the gene in S5 locus of the ten varieties was also nonfunctional and it proved the presence of S5n gene. 关键词：基因测序分子标记新种质水稻品种产量序列分析矿业杂种不育

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Mining rice new germplasm containing S_5~n gene by functional molecular marker and sequencing

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