Fine mapping of the awn gene on chromosome 4 in rice by association and linkage analyses

查看全文 作　　者：HU [1]GuangLong;ZHANG [1]DongLing;PAN [1]HuiQiao;LI [1]Ben;WU [1]JianTao;ZHOU [2]XueYa;ZHANG [1,3]QunYuan;ZHOU [1]Lei;YAO [1]GuoXin;LI [1]JunZhou;LI [1]JinJie;ZHANG [1]HongLiang;LI [1]ZiChao

机构地区：[1]Key Laboratory of Crop Genomics and Genetic Improvement of Ministry ofAgriculture, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;[2]Key Laboratory of Btomformattcs and Bioinformatics Division of Ministry of Education, Tsinghua National Laboratory for Information Sciences and Technology, Department of Automation, Tsinghua University, Beijing 100084, China;[3]Division of Statistical Genomics, Washington University School of Medicine, St Louis, MO, USA

出　　处：《Chinese Science Bulletin》索引2011年第56卷第9期,共5页

基　　金：supported by the National Key Basic Research Program of China (2010CB125904 and 2004CB117201) ;the National HighTechnology Research and Development Program of China (2006A- A10Z158) 摘　　要：Awnness is a key trait in rice domestication, yet no studies have been conducted on fine mapping or association mapping of the rice awn gene. In this study, we investigated the awnness and genotype of a core collection of 303 cultivated rice varieties and a BC5F2 segregating population of 200 individuals. Combining association and linkage analyses, we mapped the awnness related genes to chromosome 4. Primary association analysis using 24 SSR markers revealed five loci significantly associated with awnness on chromosome 4. The associated markers cover previously identified regions. Fine association mapping was conducted using another 29 markers within a 4-Mb region, covering the associated marker in34, which is close to the awn gene Awn4.1. Seven associated markers were revealed, distributed over an 870-kb region. Combining the fine association mapping and linkage analysis of awnness in the 200 BC5F2 segregating population, we finally identified a 330-kb region as the candidate region for Awn4.1. The results indicate that combining association mapping and linkage mapping provides an efficient and precise approach to both genome-wide mapping and fine mapping of rice genes. 关键词：4号染色体水稻品种连锁分析精细定位基因图谱协会 SSR标记分离群体

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Fine mapping of the awn gene on chromosome 4 in rice by association and linkage analyses

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