A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

查看全文 作　　者：ZHANG [1]Zhen;LIU [1]JingFu;YAO [2]Yan;JIANG [1]GuiBin

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100086, China;[2]Department of Isotope, China Institute of Atomic Energy, Beijing 102413, China

出　　处：《Chinese Science Bulletin》索引2011年第56卷第15期,共5页

基　　金：supported by the National Natural Science Foundation of China (21025729 and 20921063) 摘　　要：A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples,using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu 3+) and samarium (Sm 3+),respectively.The detection limits for CAP and RAC were 0.06 and 0.25ng/g.The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5ng/g,and 69.8%-85.8% for RAC at spiking levels of 1-10ng/g.The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS,with correlation coefficients (R) between 0.92-0.98. 关键词：时间分辨荧光莱克多巴胺同时测定猪组织氯霉素双标签竞争力免疫分析方法

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A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue

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