Protective effects of ACLF sera on metabolic functions and proliferation of hepatocytes co-cultured with bone marrow MSCs in vitro

查看全文 作　　者：Xiao-Lei [1,2,3,4]Shi;Jin-Yang [1,2,3,4]Gu;Yue [4,5]Zhang;Bing [1,4]Han;Jiang-Qiang [1,4]xiao;Xian-Wen [4]Yuan;Ning [6]Zhang;Yi-Tao [1,2,3,4]Ding

机构地区：[1]Department of Hepatobiliary Surgery, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China;[2]Jiangsu Provin- cial Key Medical Center for Hepatobiliary Diseases, Nanjing 210008, Jiangsu Province, China;[3]Jiangsu Key Labora- tory of Molecular Medicine, Nanjing 210008, Jiangsu Province, Jiangsu Province China;[4]Institute of Hepa- tobiliary Surgery, Nanjing University, Nanjing 210008, Jiangsu Province, China;[5]Department of Hepatobiliary Surgery, Drum Tower Clinical Medical College of Nanjing Medical University, Nan- jing 210008, Jiangsu Province, China;[6]Center for Artificial Liver Therapy, Nanjing Sec- ond Hospital, Naniing 210008, Jiangsu Province, China

出　　处：《World Journal of Gastroenterology》索引2011年第17卷第19期,共10页

基　　金：Supported by the National Natural Science Foundation of China,No.30772129;Jiangsu Provincial Key Medical Center for Hepatobiliary Disease,No.ZX200605 摘　　要：AIM:To investigate whether the function of hepatocytes co-cultured with bone marrow mesenchymal stem cells (MSCs) could be maintained in serum from acute-onchronic liver failure (ACLF) patients.METHODS:Hepatocyte supportive functions and cytotoxicity of sera from 18 patients with viral hepatitis B-induced ACLF and 18 healthy volunteers were evaluated for porcine hepatocytes co-cultured with MSCs and hepatocyte mono-layered culture,respectively.Chemokine profile was also examined for the normal serum and liver failure serum.RESULTS:Hepatocyte growth factor (HGF) and Tumor necrosis factor;tumor necrosis factor (TNF)-α were remarkably elevated in response to ACLF while epidermal growth factor (EGF) and VEGF levels were significantly decreased.Liver failure serum samples induced a higher detachment rate,lower viability and decreased liver support functions in the homo-hepatocyte culture.Hepatocytes co-cultured with MSCs could tolerate the cytotoxicity of the serum from ACLF patients and had similar liver support functions compared with the hepatocytes cultured with healthy human serum in vitro.In addition,cocultured hepatocytes maintained a proliferative capability despite of the insult from liver failure serum.CONCLUSION:ACLF serum does not impair the cell morphology,viability,proliferation and overall metabolic capacities of hepatocyte co-cultured with MSCs in vitro. 关键词：肝细胞生长因子骨髓干细胞 ACLF 体外培养人血清细胞增殖代谢功能保护作用

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Protective effects of ACLF sera on metabolic functions and proliferation of hepatocytes co-cultured with bone marrow MSCs in vitro

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