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Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment:a radioimmunoconjugate for hepatocellular carcinoma imaging

查看全文 作　　者：Hui Jie [1]Bian;Zhi Nan [1]Chen;Jing Lan [2]Deng 高影响力作者 机构地区：[1]Cell Engineering Research Center,Basic Medical.Department,Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China;[2]Department of Clinical Nuclear Medicine,Xijing Hospital,Xi’an 710033,Shaanxi Province,China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2000年第6卷第3期,共5页高影响力期刊 基　　金：National Natural Science Foundation of China,No.39700175 摘　　要：AIM To directly radiolabel an anti-hepatomamAb fragment HAb18 F(ab’)2 with 99mTc bystannous-reduced method,and assess thestability,biodistribution and radioimmun-oimaging(RⅡ).METHODS Immunoreactive fraction wasdetermined according to Lindmo’s method.Ellman’s reagent was used to determine thenumber of thiols in the reduced F(ab’)2.Labelingefficiency and homogeneity were measured bypaper chromatography,sodium dodecylsulphatepolyacrylamide gel electrophoresis(SDS-PAGE)and autoradiography.Challenge assay involvedthe incubation of aliquots of labeled antibody inethylenediaminetetraacetate( EDTA )and L-cysteine(L-cys)solutions with different molarratio at 37℃ for 1h,respectively.Investigationsin vivo utilized nude mice bearing humanhepatocellular carcinoma(HHCC)xenograftswith gamma camera imaging and tissuebiodistribution studies at regular intervals.RESULTS The labeling procedure was finishedwithin 1.5 h compared with the'pretinning'method which would take at least 21h.In vitrostudies demonstrated that the radiolabeled mAbfragment was homogeneous and retained itsimmunoreactivity.Challenge studies indicatedthat 99mTc-labeled HAb18 F(ab’)2 in EDTA is morestable than in L-cys.Imaging and biodistribution showed a significant tumor uptake at 24 h post-injection of 99mTc-labeled HAb18 F(ab’)2.Theblood,kidney,liver and tumor uptakes at 24hwere 0.56±0.09,56.45±11.36,1.43±0.27 and6.57±3.01(%ID/g),respectively.CONCLUSION 99mTc-HAb18 F(ab’)2 conjugateprepared by this direct method appears to be aneffective way to detect hepatoma in nude micemodel. 关 键 词：ANTIBODY MONOCLONAL ANTIBODY FRAGMENTS technetium-99m hepatocellular carcinoma liver neoplasms RADIOIMMUNOIMAGING
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