Establishment of Hamster-and Human-PRNP Transgenic Mice

查看全文 作　　者：GONG Han [1,2]Shi;TIAN [2]Chan;ZHANG Bao [2]Yun;WANG Zhao [2]Yun;XIE Wu [1,2]Ling;JING Yuan [2]Yuan;GAO [2]Chen;JIANG Hui [2]Ying;SHI [2]Qi;LIU [1]Yong;DONG Xiao [2]Ping

机构地区：[1]School of Medicine, Xi'an Jiao Tong University, Xi'an 710061, Shaanxi, China;[2]State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

出　　处：《Biomedical and Environmental Sciences》索引2011年第24卷第6期,共9页

基　　金：supported by Chinese National Natural Science Foundation Grants 30771914 and 30800975;Institution Technique R&D Grant (2008EG150300);National Basic Research Program of China (973 Program) (2007CB310505);China Mega-Project for Infectious Disease (2009ZX10004-101);the SKLID Development Grant (2008SKLID102 and 2008SKLID202) 摘　　要：Objective To create transgenic mice expressing hamster‐ and human‐PRNP as a model for understanding the physiological function and pathology of prion protein (PrP),as well as the mechanism of cross‐species transmission of transmissible spongiform encephalopathies (TSEs).Methods Hamster and human‐PRNP transgenic mice were established by conventional methods.The copy number of integrated PRNP in various mouse lines was mapped by real‐time PCR.PRNP mRNA and protein levels were determined by semi‐quantitative RT‐PCR,real‐time RT‐PCR,and western blot analysis.Histological analyses of transgenic mice were performed by hematoxylin and eosin (H & E) staining and immunohistochemical (IHC) methods.Results Integrated PRNP copy number in various mouse lines was 53 (Tg‐haPrP1),18 (Tg‐huPrP1),3 (Tg‐huPrP2),and 16 (Tg‐huPrP5),respectively.Exogenous PrPs were expressed at both the transcriptional and translational level.Histological assays did not detect any abnormalities in brain or other organs.Conclusion We have established one hamster‐PRNP transgenic mouse line and three human‐PRNP transgenic mouse lines.These four transgenic mouse lines provide ideal models for additional research. 关键词：转基因小鼠朊病毒人类传染性海绵状脑病 RT-PCR技术实时RT-PCR BLOT分析蛋白水平

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Establishment of Hamster-and Human-PRNP Transgenic Mice

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