Development of a Rapid Multi-residue Assay for Detecting β-lactams Using Penicillin Binding Protein 2x

查看全文 作　　者：ZENG [1]Kum;ZHANG [1]Jing;WANG [1]Yang;WANG [1]ZhanHui;ZHANG Su [1]Xia;WU Chong [1]Ming;SHEN Jian [1]Zhong

机构地区：[1]Department of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,China Agricultural University

出　　处：《Biomedical and Environmental Sciences》索引2013年第26卷第2期,共10页

摘　　要：Objective To develop a rapid multi-residue assay for detecting 16 demanded by the European Union (EU). Methods A recombinant penicillin-binding protein(PBP)2x* from Streptococcus pneumoniae R6 was expressed in vitro and six β-lactams were conjugated to HRP by four methods.A rapid multi-residue assay for β-lactams was established with PBP2x* and HRP-conjugate. Results PBP2x* was expressed and purified successfully and the ideal HRP-conjugate was identified. The multi-residue assay was developed.After optimization,penicillin G,ampicillin,amoxicillin, cloxacillin,dicloxacillin,oxacillin,nafcillin,cephalexin,ceftiofur,cefaIonium,cefquinome,cefazolin, cefoperazone,cephacetrile,and cephapirin can be detected at levels below MRL in milk with simple pretreatment. Conclusion This assay developed can detect all 16 β-lactams demanded by the European Union(EU). The whole procedure takes only 45 min and can detect 42 samples and the standards with duplicate analysis. 关键词：快速检测方法青霉素G 多残留分析结合蛋白多残留检测欧洲联盟头孢氨苄肺炎链球菌

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Development of a Rapid Multi-residue Assay for Detecting β-lactams Using Penicillin Binding Protein 2x

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