Simulated Microgravity Conditions and Carbon Ion Irradiation Induce Spermatogenic Cell Apoptosis and Sperm DNA Damage

查看全文 作　　者：LI Hong [1,2,3,4]Yan;ZHANG [1,2,3]Hong;MIAO Guo [1,2,3,4]Ying;XIE [1,2,3]Yi;SUN [1,2,3,4]Chao;DI Cui [1,2,3]Xia;LIU [1,2,3]Yang;LIU Yuan [1,2,3]Yuan;ZHANG [1,2,3]Xin;MA Xiao [1,2,3]Fei;XU [1,2,3,4]Shuai;GAN [1,2,3]Lu;ZHOU [1,2,3]Xin

机构地区：[1]Department of Heavy Ion Radiation Medicine,Institute of Modern Physics, Chinese Academy of Sciences;[2]Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences;[3]Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province;[4]University of Chinese Academy of Sciences

出　　处：《Biomedical and Environmental Sciences》索引2013年第26卷第9期,共9页

基　　金：supported by the Knowledge Innovation Project of the Chinese Academy of Sciences(KJCX2-YW-L08);the National Basic Research Program of China(2010CB834202);the National Natural Science Foundation of China(10835011);the Scientific Technology Research Projects of Gansu Province(0702NKDA045,0806RJYA020) 摘　　要：Objective To investigate the effect of simulated microgravity and carbon ion irradiation(CIR) on spermatogenic cell apoptosis and sperm DNA damage to the testis of male Swiss Webster mice,and assess the risk associated with space environment.Methods Sperm DNA damage indicated by DNA fragmentation index(DFI) and high DNA stainability(HDS) was measured by sperm chromatin structure assay(SCSA).Apoptosis of spermatogenic cells was detected by annexin V-propidium iodide assay.Bax(the expression levels of p53) and proliferating cell nuclear antigen(PCNA) were measured by immunoblotting;p53 and PCNA were located by immunohistology.Results HDS,DFI,apoptosis index,and the expression levels of p53 and Bax were detected to be significantly higher in the experimental groups(P<0.05) compared with those in the control group;however,the PCNA expression varied to a certain degree.p53-and PCNA-positive expression were detected in each group,mainly in relation to the spermatogonic cells and spermatocytes.Conclusion The findings of the present study demonstrated that simulated microgravity and CIR can induce spermatogenic cell apoptosis and sperm DNA damage.Sperm DNA damage may be one of the underlying mechanisms behind male fertility decline under space environment.These findings may provide a scientific basis for protecting astronauts and space traveler's health and safety. 关键词：生精细胞凋亡 DNA损伤精子染色体模拟微重力辐照诱导碳离子微重力条件 p53基因

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Simulated Microgravity Conditions and Carbon Ion Irradiation Induce Spermatogenic Cell Apoptosis and Sperm DNA Damage

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