Molecular Cytogenetic Characterization of a Wheat-Leymus mollis 3D(3Ns) Substitution Line with Resistance to Leaf Rust

查看全文 作　　者：Yuhui [1]Pang;Xinhong [1]Chen;Jixin [1]Zhao;Wanli [1]Du;Xueni [2]Cheng;Jun [1]Wu;Yanli [1]Li;Liangming [1]Wang;Jing [1]Wang;Qunhui [1]Yang

机构地区：[1]College of Agronomy,Northwest A&F University;[2]College of Life Sciences,Northwest A&F University

出　　处：《Journal of Genetics and Genomics》索引2014年第41卷第4期,共10页

基　　金：supported by the National High Technology Research and Development Program of China (863 Program) (Grant No.2011AA100501);the Major Innovation Project for Science and Technology of Shaanxi Province (Grant No.2011KTZB02-01);the Basic Scientific Research Project in Central Universities (Grant No.QN2011001);the Tang Zhong-Ying Breeding Funding Project at the Northwest A&F University in China 摘　　要：Leymus mollis(Trin.)Pilger(NsNsXmXm,2n?28),a wild relative of common wheat,possesses many potentially valuable traits that could be transferred to common wheat during breeding programs.In this study,the karyotypic constitution of a wheat e L.mollis 3D(3Ns#1)disomic substitution line isolated from the F5progeny of octoploid Tritileymus M842-16 Triticum durum cv.D4286,which was designated as 10DM57,was determined using genomic in situ hybridization(GISH),fluorescent in situ hybridization(FISH),SSR markers,and ESTSTS markers.Screening of mitosis and meiosis showed that 10DM57 had a chromosome karyotype of 2n?42?21II.GISH indicated that10DM57 was a line with 40 chromosomes from wheat and two of the Ns chromosomes from L.mollis,which formed a ring bivalent in pollen mother cells at metaphase I.FISH analysis showed that the chromosome 3D may be replaced by 3Ns#1 in 10DM57.DNA markers,including SSR and EST-STS primers,showed that the pair of wheat chromosome 3D in 10DM57 was substituted by the pair of chromosome 3Ns#1from L.mollis.Evaluation of the agronomic traits showed that,compared with its common wheat relative 7182,10DM57 was resistant to leaf rust while the spike length and number of spikes per plant were improved significantly,which correlated with a higher wheat yield.The new germplasm,10DM57,could be exploited as an intermediate material in wheat genetic and breeding programs. 关键词：分子细胞遗传学普通小麦抗叶锈病 3D 基因组原位杂交代换系染色体核型 SSR标记

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Molecular Cytogenetic Characterization of a Wheat-Leymus mollis 3D(3Ns) Substitution Line with Resistance to Leaf Rust

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