Immunocytochemical Study of Cells in the Vitreous of Proliferative Vitreoretretinopathy

查看全文 作　　者：Bing Liu, Yannian Hui, Jixian Ma, Haiyang DuDepartment of Ophthalmology, Xijing Hospital, The Fourth Military Medical University, Xian 710032 , China 出　　处：《眼科学报》索引1999年第15卷第1期,共4页 摘　　要：Purpose: To identify the cellular components of vitreous samples obtained during vit-rectomy for proliferative vitreoretinopathy(PVR).Methods: With the use of three intermediate filament (IF) proteins, vimentin, glialfibrillary acidic protein (GFAP), and cytokeratin (CK), cytocentrifuge slides of 14fresh vitreous aspirates were detected with immunohistochemical technique.Results: All the specimens contained epithelial-like proliferative cells with or withoutpigment and some membrane-like pieces. Immunocytochemical staining showed that76.0-90.0% cells stained for CK, 17.4-29.6% cells expressed GFAP, and 80.1-91.0% cells were positive for vimentin.Conclusions : Majority of cells in the vitreous samples originated from retinal pigmentepithelial cells (RPE) and glial cells in PVR. Expression of IF proteins may be determinedby tissue of origin and local microenvironment. Eye Science 1999 ; 15; 13 - 16. 关 键 词：眼 失明 媒介细丝蛋白 免疫细胞化学 视网膜色素上皮细胞 玻璃质视网膜病