In vivo tracking of human adipose-derived stem cells labeled with ferumoxytol in rats with middle cerebral artery occlusion by magnetic resonance imaging

查看全文 作　　者：Yan [1]Yin;Xiang [2]Zhou;Xin [3]Guan;Yang [4]Liu;Chang-bin [2]Jiang;Jing [4]Liu

机构地区：[1]Department of Neurology,the Second Affiliated Hospital of Dalian Medical University;[2]Department of Neurology,the First Affiliated Hospital of Dalian Medical University;[3]College of Life Sciences,Liaoning Normal University;[4]Regenerative Medicine Center,First Affiliated Hospital of Dalian Medical University

出　　处：《Neural Regeneration Research》索引2015年第10卷第6期,共7页

基　　金：supported by the Science and Technology Plan Project of Dalian City in China,No.2014E14SF186 摘　　要：Ferumoxytol, an iron replacement product, is a new type of superparamagnetic iron oxide approved by the US Food and Drug Administration. Herein, we assessed the feasibility of tracking transplanted human adipose-derived stem cells labeled with ferumoxytol in middle cerebral artery occlusion-injured rats by 3.0 T MRI in vivo. 1 × 104 human adipose-derived stem cells labeled with ferumoxytol-heparin-protamine were transplanted into the brains of rats with middle cerebral artery occlusion. Neurologic impairment was scored at 1, 7, 14, and 28 days after transplantation. T2-weighted imaging and enhanced susceptibility-weighted angiography were used to observe transplanted cells. Results of imaging tests were compared with results of Prussian blue staining. The modified neurologic impairment scores were significantly lower in rats transplanted with cells at all time points except 1 day post-transplantation compared with rats without transplantation. Regions with hypointense signals on T2-weighted and enhanced susceptibility-weighted angiography images corresponded with areas stained by Prussian blue, suggesting the presence of superparamagnetic iron oxide particles within the engrafted cells. Enhanced susceptibility-weighted angiography image exhibited better sensitivity and contrast in tracing ferumoxytol-heparin-protamine-labeled human adipose-derived stem cells compared with T2-weighted imaging in routine MRI. 关键词：磁共振成像脂肪干细胞细胞标记大鼠大脑跟踪闭塞动脉

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In vivo tracking of human adipose-derived stem cells labeled with ferumoxytol in rats with middle cerebral artery occlusion by magnetic resonance imaging

参考文献(65)

引证文献(7)

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