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Changes in Growth, Carbon and Nitrogen Enzyme Activity and mRNA Accumulation in the Halophilic Microalga Dunaliella viridis in Response to NaCl Stress

查看全文 作　　者：WANG [1]Dongmei;WANG [1]Weiwei;XU [1]Nianjun;SUN [1]Xue 高影响力作者 机构地区：[1]Key Laboratory of Applied Marine Biotechnology of Ministry of Education, School of Marine Sciences,Ningbo University, Ningbo 315211, P. R. China高影响力机构 出　　处：《Journal of Ocean University of China》索引2016年第15卷第6期,共7页高影响力期刊 基　　金：funded by the Project of Science and Technology Innovation Team of Zhejiang Province (2010R50025-25);sponsored by the K. C. Wong Magna Fund 摘　　要：Many species of microalga Dunaliella exhibit a remarkable tolerance to salinity and are therefore ideal for probing the effects of salinity. In this work, we assessed the effects of NaCl stress on the growth, activity and m RNA level of carbon and nitrogen metabolism enzymes of D. viridis. The alga could grow over a salinity range of 0.44 mol L^(-1) to 3.00 mol L^(-1) NaCl, but the most rapid growth was observed at 1.00 mol L^(-1) NaCl, followed by 2.00 mol L^(-1) NaCl. Paralleling these growth patterns, the highest initial and total Rubisco activities were detected in the presence of 1.00 mol L^(-1) NaCl, decreasing to 37.33% and 26.39% of those values, respectively, in the presence of 3.00 mol L^(-1) Na Cl, respectively. However, the highest extracellular carbonic anhydrase(CA) activity was measured in the presence of 2.00 mol L^(-1) NaCl, followed by 1.00 mol L^(-1) NaCl. Different from the two carbon enzymes, nitrate reductase(NR) activity showed a slight change under different NaCl concentrations. At the transcriptional level, the m RNAs of Rubisco large subunit(rbcL), and small subunit(rbc S), attained their highest abundances in the presence of 1.00 and 2.00 mol L^(-1)NaCl, respectively. The CA mRNA accumulation was induced from 0.44 mol L^(-1) to 3.00 mol L^(-1) NaCl, but the NR m RNA showed the decreasing tendency with the increasing salinity. In conclusion, the growth and carbon fixation enzyme of Rubisco displayed similar tendency in response to NaCl stress, CA was proved be salt-inducible within a certain salinity range and NR showed the least effect by NaCl in D. viridis. 关 键 词：NACL胁迫 MRNA水平 杜氏盐藻 酶活性 活性碳 Rubisco活性 经济增长 积累
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