Salvianolate Reduces Murine Myocardial Ischemia and Reperfusion Injury via ERK1/2 Signaling Pathways in vivo

查看全文 作　　者：QI Jian-[1]yong;YU [2]Juan;HUANG Dong-[1]hui;GUO Li-[1]heng;WANG [1]Lei;HUANG [1]Xin;HUANG Hai-[2]ding;ZHOU [3]Miao;ZHANG Min-[1]zhou;Jiashin [4]Wu

机构地区：[1]Intensive Care Laboratory,Guangdong Province Hospital of Chinese Medicine,2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China;[2]Animal Laboratory,Guangdong Province Hospital of Chinese Medicine,2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;[3]Department of Oral and Maxillary Surgery,Stomatology Hospital of Guangzhou Medical University,Guangzhou 510006,China;[4]Northeast Ohio Medical Universitv.Rootstown.Ohio.USA

出　　处：《Chinese Journal of Integrative Medicine》索引2017年第23卷第1期,共8页

基　　金：Supported by National Natural Science Foundation of China(No.81473471 and No.81573708);Foundation of Guangdong Hospital of Chinese Medicine(No.YK2013B2N11,No.YN2014ZH01,and No.YN2014ZHR203) 摘　　要：Objective:To analyze the effects of salvianolate on myocardial infarction in a murine in vivo model of ischemia and reperfusion(l/R) injury.Methods:Myocardial l/R injury model was constructed in mice by 30 min of coronary occlusion followed by 24 h of reperfusion and pretreated with salvianolate 30 min before l/R(SAL group).The SAL group was compared with SHAM(no l/R and no salvianolate),l/R(no salvianolate),and ischemia preconditioning(IPC) groups.Furthermore,an ERK1/2 inhibitor PD98059(1 mg/kg),and a phosphatidylinositol-3-kinase(PI3-K) inhibitor,LY294002(7.5 mg/kg),were administered intraperitoneal injection(i.p) for 30 min prior to salvianolate,followed by l/R surgery in LY and PD groups.By using a double staining method,the ratio of the infarct size(IS) to left ventricle(LV) and of risk region(RR) to LV were compared among the groups.Correlations between IS and RR were analyzed.Western-blot was used to detect the extracellular signal-regulated kinase 1/2(ERK1/2) and protein kinase B(AKT) phosphorylation changes.Results:There were no significant differences between RR to LV ratio among the SHAM,l/R,IPC and SAL groups(P>0.05).The SAL and IPC groups had IS of 26.1%± 1.4%and 22.3%±2.9%of RR,respectively,both of which were significantly smaller than the l/R group(38.5%±2.9%of RR,P<0.05,P<0.01,respectively).Moreover,the phosphorylation of ERK1/2 was increased in SAL group(P<0.05),while AKT had no significant change.LY294002 further reduced IS,whereas the protective role of salvianolate could be attenuated by PD98059,which increased the IS.Additionally,the IS was not linearly related to the RR(r=0.23,0.45,0.62,0.17,and 0.52 in the SHAM,l/R,SAL,LY and PD groups,respectively).Conclusion:Salvianolate could reduce myocardial l/R injury in mice in vivo,which involves an ERK1/2 pathway,but not a PI3-K signaling pathway. 关键词：丹参多酚酸盐脑缺血再灌注 ERK1/2 信号通路损伤模型心肌缺血小鼠 PD98059

相关文献

参考文献（32）
引证文献（18）
耦合文献（0）

参考文献(32)

引证文献(18)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Salvianolate Reduces Murine Myocardial Ischemia and Reperfusion Injury via ERK1/2 Signaling Pathways in vivo

参考文献(32)

引证文献(18)

用户提示