Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures

查看全文 作　　者：Ruchao [1,2]Peng;Ying [1,3]Xu;Tengfei [2,4]Zhu;Ningning [5]Li;Jianxun [1,2]Qi;Yan [1]Chai;Min [6]Wu;Xinzheng [7]Zhang;Yi [1,2,8,9]Shi;Peiyi [10]Wang;Jiawei [11]Wang;Ning [5]Gao;George Fu [1,2,4,8,9,12]Gao

机构地区：[1]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;[2]University of Chinese Academy of Sciences, Beijing 101408, China;[3]School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China;[4]Research Network of Immunity and Health (RNIH), Beijing Institute of Life Science, Chinese Academy of Sciences, Beijing 100101, China;[5]State Key Laboratory of Membrane Biology, Peking-Tsinghua Center for Life Sciences, School of Life Sciences, Peking University, Beijing 100871, China;[6]Department of Bio- medical Sciences, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, ND 58203, USA;[7]National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;[8]Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen, Guangdong 518112, China;[9]Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China;[10]Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK;[11]State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China;[12]National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Con- trol and Prevention (China CDC), Beijing 102206, China

出　　处：《Cell Research》索引2017年第27卷第7期,共12页

基　　金：We thank all staffs at the National Center for Protein Science Shanghai (NCPSS) cryo-EM department and Xiaojun Huang, Gang Ji and other staffs at the Center of Biological Imaging (IBP, Chinese Academy of Sciences (CAS)) for assistance with data collection. We are grateful to Dr Guopeng Wang (The Core Fa- cilities at School of Life Sciences, Peking University) and Dr Tie Yang (The State Key Laboratory of Membrane Biology, Institute of Zoology, CAS, Beijing) for technical support in electron mi- croscope operation. This study was supported by the Strategic Priority Research Program of CAS (XDB08020100), the Ministry of Science and Technology of China (973 Project; 2013CB531502 and 2014CB542503). YS is supported by the Excellent Young Sci- entist Program from the National Natural Science Foundation of China (NSFC; 81622031), the Excellent Young Scientist Program of CAS and the Youth Innovation Promotion Association CAS (20 15078). GFG is supported partly as a leading principal investi- gator of the NSFC Innovative Research Group (81621091). 摘　　要：抗菌素编码 anti-CRISPR suppressors 抵抗他们的细菌的主人的 CRISPR/Cas 免疫,因此便于他们的幸存和复制。以前的研究显示出那二编码噬菌体的 anti-CRISPR 蛋白质, AcrF1 和 AcrF2,如果由由 Csy 监视建筑群阻止目标 DNA 识别的 Pseudomonas aeruginosa 的 CRISPR/Cas 系统,而是精确位于下面机制是未知的,压制类型。这里我们在场 Csy 建筑群一定的 AcrF1/2 的结构由 cryo 他们决定了单个粒子的重建。由结构的分析,我们发现 AcrF1 由防碍在 DNA 目标海滨和 crRNA 分隔符之间的基础配对禁止 Csy 建筑群的目标 DNA 识别。另外,当个别地工作或与 AcrF2 合作时, AcrF1 的多重拷贝与不同模式绑在 Csy 建筑群,它可能排除通过不同机制有约束力的目标 DNA。和以前的报告,我们为二 anti-CRISPR suppressors, AcrF1 和 AcrF2 提供一种全面工作情形,它由指向 Csy 监视建筑群的沉默 CRISPR/Cas 免疫。 关键词：CRISPR/Cas 系统 anti-CRISPR 蛋白质噬菌体有免疫力的避免 cryo 他们

相关文献

参考文献（44）
引证文献（3）
耦合文献（15）

参考文献(44)

引证文献(3)

耦合文献(15)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Alternate binding modes of anti-CRISPR viral suppressors AcrF1/2 to Csy surveillance complex revealed by cryo-EM structures

参考文献(44)

引证文献(3)

耦合文献(15)

用户提示