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IPA1 functions as a downstream transcription factor repressed by D53 in strigolactone signaling in rice

查看全文 作  者:Xiaoguang [1]Song;Zefu [1,5]Lu;Hong [1]Yu;Gaoneng [1,2]Shao;Jinsong [1,6]Xiong;Xiangbing [1]Meng;Yanhui [1]Jing;Guifu [1]Liu;Guosheng [1,7]Xiong;Jingbo [1]Duan;Xue-Feng [3]Yao;Chun-Ming [3]Liu;Hongqing [4]Li;Yonghong [1]Wang;Jiayang [1,2]Li 高影响力作者 机构地区:[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;[2]University of Chinese Academy of Sciences, Beijing 100049, China;[3]Key Laboratory of Plant Molecular Physiology, Institute of Botanv, Chinese Academy of Sciences, Beijing 100093, China;[4]Guangdong Provincial Key Lab of Biotechnology for Plant Development, South China Normal University, Guangzhou, Guangdong 510631, China;[5]Department of Genetics, University of Georgia, Athens,Georgia 30605, USA;[6]College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;[7]Agricultural Genome Institute at Shenzhen, China Academy of Agricultural Science, Shenzhen, Guangdong 518120, China高影响力机构 出  处:《Cell Research》索引2017年第27卷第9期,共14页高影响力期刊 基  金:We thank Prof Shouyi Chen (Institute of Genetics and Devel- opmental Biology, Chinese Academy of Science) for providing the plasmids GAL4BD, 35sLUC and pRTL, Prof Qian Qian and Dr Xingming Hu (State Key Laboratory of Rice Biology, China Na- tional Rice Research Institute) for providing the rice miRNA156 overexpressing lines, Prof Chuanyou Li for sharing the Plant Imaging System (Berthold Technologies), and lab members (Drs Lei Wang, Liang Jiang, Xue Liu, Huihui Liu) for their technical assistance, and Dr Bing Wang for comments on the manuscript. We are grateful to the Genomics and Bioinformatics Facility of State Key Laboratory of Plant Genomics, Institute of Genetic and Developmental Biology for phosphor imaging. This work was supported by grants from the National Key Research and Devel- opment Program of China (Grant 2016YFD0101800), National Natural Science Foundation of China (Grant 91635301), and the Strategic Priority Research Program "Molecular Mechanism of Plant Growth and Development" (Grant XDPB0401). 摘  要:Strigolactones (SL ) ,一组类胡萝卜素导出 terpenoid 内酯,是压制射击由禁止腋的芽的长出分叉的 root-to-shoot 植物激素。矮子 53 (D53 ) ,表明小径的 SL 的关键抑压者,被推测调整 SL 反应的下游的 transcriptional 网络。然而, D53 指向的下游的抄写因素还都没被报导。这里,我们报导那理想的植物体系结构 1 (IPA1 ) ,在米饭的植物体系结构的一个关键管理者,在调整 tiller 数字和导致 SL 的基因表示作为 D53 的一个直接下游的部件工作。我们证明 D53 在 vivo 并且在 vitro 与 IPA1 交往并且压制 IPA1 的 transcriptional 激活活动。我们进一步证明 IPA1 能直接绑在 D53 倡导者并且在导致 SL 的 D53 表示的反馈规定起一个关键作用。这些调查结果表明 IPA1 是可能的与 D53 行动到的长推测的抄写因素之一调停在米饭的调整 SL 的 tiller 发展。 关 键 词:转录因子 萜类内酯 信号通路 水稻 有效分蘖数 转录激活活性 理想株型 类胡萝卜素
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