5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers

查看全文 作　　者：Wenshuai [1]Li;Xu [2]Zhang;Xingyu [3,4]Lu;Lei [5]You;Yanqun [4]Song;Zhongguang [1]Luo;Jun [1]Zhang;Ji [3]Nie;Wanwei [1]Zheng;Diannan [1]Xu;Yaping [6]Wang;Yuanqiang [6]Dong;Shulin [7]Yu;Jun [6]Hong;Jianping [8]Shi;Hankun [6]Hao;Fen [6]Luo;Luchun [6]Hua;Peng [7]Wang;Xiaoping [9]Qian;Fang [10,11]Yuan;Lianhuan [11]Wei;Ming [5]Cui;Taiping [5]Zhang;Quan [5]Liao;Menghua [5]Dai;Ziwen [5]Liu;Ge [5]Chen;Katherine [12]Meckel;Sarbani [12]Adhikari;Guifang [11,13]Jia;Marc B [12]Bissonnette;Xinxiang [10,11]Zhang;Yupei [5]Zhao;Wei [14]Zhang;Chuan [3,10]He;Jie [1,15]Liu

机构地区：[1]Department of Digestive Diseases, Huashan Hospital Fudan University, Shanghai 200040, China;[2]Section of Hematology/Oncology, Department of Medicine, University of Illinois, Chicago, IL 60612, USA;[3]Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, Chicago, IL 60637, USA;[4]Shanghai Epican Genetech, Co. Ltd., Zhangjiang Hi-Tech Park, Shanghai 201203, China;[5]Department of General Surgery, Peking Union Medical College Hospital Chinese Academy of Medical Sciences, Beijing 100730, China;[6]Department of General Surgery, Huashan Hospital Fudan University, Shanghai 200040, China;[7]Department of Integrative Oncology, Shanghai Cancer Center, Fudan University, Shanghai 200032, China;[8]Department of Digestive Diseases, Pudong Hospital Fudan University, Shanghai 201399, China;[9]Department of Oncology, Nanjing Drum Tower Hospital Nanjing University Medical School Nanjing 210008, China;[10]Beijing National Laboratory for Molecular Sciences, College of Chemistry, Pelting University, Beijing 100871, China;[11]Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing 100871, China;[12]Department of Medicine, The University of Chicago, Chicago, 1L 60637, USA;[13]Department of Chemical Biology, Structure and Function Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;[14]Department of Preventive Medicine and The Robert H. Lurie Comprehensive Cancer Center, Northwestern Uni- versity Feinberg School of Medicine, Chicago, IL 60611, USA;[15]Department of Immunology, State Key Laboratory of Genetic Engi- neering, Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China

出　　处：《Cell Research》索引2017年第27卷第10期,共15页

摘　　要：象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 关键词：5-甲基胞嘧啶 DNA修饰生物标志物人类基因组羟甲基癌症诊断签名

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5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers

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