Metabolite variation in hybrid corn grain from a large-scale multisite study

查看全文 作　　者：Mingjie [1,2]Chen;R.Shyama Prasad [2,3]Rao;Yiming [2]Zhang;Cathy [4]Zhong;Jay [2]J.Thelen

机构地区：[1]Haixia Institute of Science and Technology,Horticulture Biology and Metabolomics Center,Fujian Agriculture and Forestry University;[2]Department of Biochemistry,Christopher S.Bond Life Science Center,University of Missouri;[3]Biostatistics and Bioinformatics Division,Yenepoya Research Center,Yenepoya University;[4]Regulatory Group,Du Pont Experimental Station

出　　处：《The Crop Journal》索引2016年第4卷第3期,共11页

基　　金：financially supported by DuP ont–Pioneer HiB red 摘　　要：Metabolite composition is strongly affected by genotype,environment,and interactions between genotype and environment,although the extent of variation caused by these factors may depend upon the type of metabolite.To characterize the complexity of genotype,environment,and their interaction in hybrid seeds,50 genetically diverse non-genetically modified(GM) maize hybrids were grown in six geographically diverse locations in North America.Polar metabolites from 553 harvested corn grain samples were isolated and analyzed by gas chromatography–mass spectrometry and 45 metabolites detected in all samples were used to generate a data matrix for statistical analysis.There was moderate variation among biological replicates and across genotypes and test sites.The genotype effects were detected by univariate and Hierarchical clustering analyses(HCA) when environmental effects were excluded.Overall,environment exerted larger effects than genotype,and polar metabolite accumulation showed a geographic effect.We conclude that it is possible to increase seed polar metabolite content in hybrid corn by selection of appropriate inbred lines and growing regions. 关键词：CORN GRAIN GENOTYPE Environment GC–MS Metabolomics

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Metabolite variation in hybrid corn grain from a large-scale multisite study

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