A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis

查看全文 作　　者：Xiaohan [1,2]Wang;Changxin [1]Feng;LiLi [1]Tian;Congcong [1]Hou;Wang [1,3]Tian;Bin [2]Hu;Qian [1]Zhang;Zhijie [1]Ren;Qi [1]Niu;Jiali [1]Song;Dongdong [1]Kong;Liangyu [1]Liu;Yikun [1]He;Ligeng [1]Ma;Chengcai [2]Chu;Sheng [3]Luan;Legong [1]Li

机构地区：[1]College of Life Sciences,Capital Normal University,Beijing 100048,China;[2]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,the Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China;[3]Department of Plant and Microbial Biology,University of California at Berkeley,Berkeley,CA 94720,USA

出　　处：《Molecular Plant》索引2021年第14卷第5期,共13页

基　　金：supported by grants from the Key Program of the National Natural Science Foundation of China(31930010 to L.L.);the General Program of National Natural Science Foundation of China(no.31872170 to L.L.and no.31900234 to C.H.);the National Key Research and Development Program of China(YFD0300102-3 to L.L.);the Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds(19530050165 to L.L.).;supported,in part,by a grant from the National Science Foundation(MCB-1714795 to S.L.). 摘　　要：Nitrate-induced Ca^(2+) signaling is crucial for the primary nitrate response in plants.However,the molecular mechanism underlying the generation of the nitrate-specific calcium signature remains unknown.We report here that a cyclic nucleotide-gated channel(CNGC)protein,CNGC15,and the nitrate transceptor(NRT1.1)constitute a molecular switch that controls calcium influx depending on nitrate levels.The expression of CNGC15 is induced by nitrate,and its protein is localized at the plasma membrane after establishment of young seedlings.We found that disruption of CNGC15 results in the loss of the nitrate-induced Ca^(2+) signature(primary nitrate response)and retards root growth,reminiscent of the phenotype observed in the nrt1.1 mutant.We further showed that CNGC15 is an active Ca^(2+)-permeable channel that physically interacts with the NRT1.1 protein in the plasma membrane.Importantly,we discovered that CNGC15-NRT1.1 interaction silences the channel activity of the heterocomplex,which dissociates upon a rise in nitrate levels,leading to reactivation of the CNGC15 channel.The dynamic interactions between CNGC15 and NRT1.1 therefore control the channel activity and Ca^(2+) influx in a nitrate-dependent manner.Our study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor-channel complex assembly to couple nutrient status to a specific Ca^(2+) signature. 关键词：primary nitrate response nutrient sensing calcium signature nitrate transceptor calcium channel

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A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis

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