C-reactive protein functions as a negative regulator of macrophage activation induced by apoptotic DNA

查看全文 作　　者：Weijuan [1]Zhang;Yanxing [1]Cai;Wei [1]Xu;Sidong [1,2]Xiong 机构地区：[1]Institute for Immunobiology and Department of Immunology,Shanghai Medical College,Fudan University,Shanghai 200032,China;[2]Jiangsu Key Laboratory of Infection and Immunity,Institutes of Biology and Medical Sciences,Soochow University,Suzhou 215123,China 出　　处：《Protein & Cell》索引2011年第2卷第8期,共8页 基　　金：supported by grants from the National Natural Science Foundation of China(Grant Nos.30890141,31100629,and 30671952);Shanghai STC grant(No.10JC1401400);The Program for Outstanding Medical Academic Leaders of Shanghai(Grant No.LJ06011);Jiangsu“Pan-Deng”Project(Grant No.BK2010004);Postdoctoral Science Foundation Funded Project of China(Grant No.20100480538);Shanghai(Grant No.11R21411000);The Research Fellow Fund and Startup Fund for Young Backbone Scholars of Shanghai Medical College,Fudan University. 摘　　要：C-reactive protein(CRP),an acute-phase protein with an ability to bind to nuclear antigen,has been reported to regulate cytokine secretion and modulate immune responses.We previously reported that activated syngeneic lymphocyte-derived apoptotic DNA(apopDNA)could induce macrophage activation and contribute to the initiation and progression of lupus nephritis.It is reasonable to hypothesize that CRP might regulate apopDNA-induced macrophage activation.Herein,CRP was shown to promote macrophage-mediated apopDNA uptake by binding to apopDNA(CRP/apopDNA complex).Notably,CRP/apopDNA treatment inhibited the production of inflammatory cytokines and chemokines by macrophages which could be induced by apopDNA alone.Further coculture and transwell studies revealed that CRP/apopDNA-induced macrophages prohibited apopDNA-induced macrophage activation in an IL-10 dependent manner.These results provide insight into the potential mechanism of CRP regulatory activity in macrophage activation induced by apopDNA in the context of lupus nephritis and other autoimmune diseases. 关 键 词：C-reactive protein(CRP) macrophage activation AUTOIMMUNITY systemic lupus erythematosus(SLE) lupus nephritis