Pigsties near dwellings as a potential risk factor for the prevalence of Japanese encephalitis virus in adult in Shanxi,China

查看全文 作　　者：Xiaojie [1]Ren;Shihong [2,3]Fu;Peifang [4]Dai;Huanyu [2,3]Wang;Yuanyuan [1]Li;Xiaolong [2,3]Li;Wenwen [2,3]Lei;Xiaoyan [2,3]Gao;Ying [2,3]He;Zhi [2,3]Lv;Jingxia [4]Cheng;Guiqin [1]Wang;Guodong [2,3]Liang

机构地区：[1]Department of Immunology and Microbiology,Shanxi Medical University,Taiyuan 030001,China;[2]State Key Laboratory of Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;[3]Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Hangzhou 310058,China;[4]Shanxi Center for Disease Control and Prevention,Taiyuan 030001,China

出　　处：《Infectious Diseases of Poverty》索引2017年第6卷第1期,共11页

基　　金：This work was supported by grants from National Natural Science Foundation of China(81290342 and 81501757);Development Grant of State Key Laboratory of Infectious Disease Prevention and Control(2014SKLID103). 摘　　要：Background:The increasing trend of adult cases of Japanese encephalitis(JE)in China,particularly in northern China,has become an important public health issue.We conducted an epidemiological investigation in the south of Shanxi Province to examine the relationships between mosquitoes,Japanese encephalitis virus(JEV),and adult JE cases.Methods:Mosquito specimens were collected from the courtyards of farmers’households and pig farms in Shanxi Province.Mosquitoes were pooled,homogenized,and centrifuged.Reverse transcription-polymerase chain reaction(RT-PCR)was used to detect mosquito-borne arbovirus genes in homogenates.Specimens positive for these genes were inoculated into the baby hamster kidney cell line(BHK-21)to isolate virus.Minimum infection rate was calculated and phylogenetic analyses were performed.Results:A total of 7943 mosquitoes belonging to six species in four genera were collected;Culex tritaeniorhynchus accounted for 73.08%(5805/7943),C.pipiens pallens for 24.75%(1966/7943),and the remaining 3%(104/7943)consisted of Anopheles sinensis,Aedes vexans,Ae.dorsalis,and Armigeres subalbatus.Sixteen pools were positive for JEV based on RT-PCR using JEV pre-membrane gene nested primers.Phylogenetic analyses showed that all JEVs belonged to genotype I;two pools were positive using Getah Virus(GETV)gene primers.In addition,one JEV strain(SXYC1523)was isolated from C.pipiens pallens specimens.These results indicate that the minimum infection rate of JEV in mosquito specimens collected from the courtyards of farmers’households with pigsties was 7.39/1000;the rate for pig farms was 2.68/1000;and the rate for farmers’courtyards without pigsties was zero.Conclusions:The high-prevalence regions of adult JE investigated in this study are still the natural epidemic focus of JEV.Having pigsties near dwellings is a potential risk factor contributing to the prevalence of adult JE.To prevent the occurrence of local adult JE cases,a recommendation was raised that,besides continuing to implement the Expanded Program on Immunization for children,the government should urge local farmers to cease raising pigs in their own courtyards to reduce the probability of infection with JEV. 关键词：Adult Japanese encephalitis Epidemic disease Mosquito-borne arbovirus Japanese encephalitis virus

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Pigsties near dwellings as a potential risk factor for the prevalence of Japanese encephalitis virus in adult in Shanxi,China

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