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A COVID-19 T-Cell Response Detection Method Based on a Newly Identified Human CD8+T Cell Epitope from SARS-CoV-2—Hubei Province,China,2021

查看全文 作　　者：Jie [1]Zhang;Dan [2]Lu;Min [1]Li;Maoshun [3]Liu;Sijia [3]Yao;Jianbo [4]Zhan;William [1]J.Liu;George [1,2,3]F.Gao 高影响力作者 机构地区：[1]NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,China;[2]Savaid Medical School,University of Chinese Academy of Sciences,Beijing,China;[3]College of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,Zhejiang,China;[4]Hubei Provincial Center for Disease Control and Prevention,Wuhan,Hubei,China高影响力机构 出　　处：《China CDC weekly》索引2022年第4卷第5期,共6页高影响力期刊 基　　金：Supported by the National Natural Science Foundation of China(82161148008 and 81971501);the Major Special Projects for Infectious Disease Research of China(2018ZX10101002-005);CAMS Research Units of Adaptive Evolution and Control of Emerging Viruses(2018RU009);W.J.L.is supported by the Excellent Young Scientist Program of the National Natural Science Foundation of China(81822040). 摘　　要：Introduction:Similar to antibody detection,severe acute respiratory syndrome coronavirus type 2(SARSCoV-2)-specific T-cell response evaluation is also pivotal among the coronavirus disease 2019(COVID-19)convalescents and the vaccinated populations.Nucleocapsid(N)protein is one of the main structural proteins of SARS-CoV-2 and can trigger T-cell responses in humans.Methods:An overlapping peptide pool covering the full length of the N protein was designed,peptides with positive T-cell activating potency in COVID-19 convalescents were screened,and CD8^(+)T cell epitopes were further identified.The epitope was used to detect the SARS-CoV-2-specific CD8^(+)T cell responses in COVID-19 convalescents based in intracellular cytokine staining and tetramer staining in flow cytometry.Results:A human leukocyte antigen A(HLAA)*1101-restricted CD8^(+)T cell epitope,which could stimulate the production of IFN-γvia peripheral blood mononuclear cells(PBMCs)of the convalescents was defined,and the tetramer generated with this epitope could detect SARS-CoV-2-specific T cells in the PBMCs of the convalescents.The structural investigation eliminated that the epitope was a typical HLA-A^(*)1101-restricted T-cell epitope which was conserved among all the sarbecoviruses.Discussion:The newly identified SARS-CoV-2-derived T-cell epitope was helpful to detect the cellular immunity against different sarbecoviruses including SARS-CoV and SARS-CoV-2.This study provided an evaluation method and also a peptide candidate for the research and development of T-cell based vaccine for the virus. 关 键 词：RESTRICTED IMMUNITY HUMAN
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