维普中文期刊产品整合服务

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

查看全文 作　　者：Zhi [1]Chen;Jingfeng [1,2]Li;Tianzhong [1,2]Li;Taojian [1]Fan;Changle [1]Meng;Chaozhou [1]Li;Jianlong [1]Kang;Luxiao [1]Chai;Yabin [1,5]Hao;Yuxuan [1,6]Tang;Omar AAl-[7]Hartomy;Swelm [7]Wageh;Abdullah GAl-[8,9]Sehemi;Zhiguang [10]Luo;Jiangtian [2]Yu;Yonghong [11]Shao;Defa [12]Li;Shuai [13]Feng;William [14,15]J.Liu;Yaqing [16]He;Xiaopeng [4]Ma;Zhongjian [3]Xie;Han [1]Zhang 高影响力作者 机构地区：[1]Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics,International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education,Shenzhen Institute of Translational Medicine,Department of Otolaryngology,Shenzhen Second Peoples Hospital,the First Affiliated Hospital,Institute of Microscale Optoelectronics,Shenzhen University,Shenzhen 518060,China;[2]Shenzhen International Institute for Biomedical Research,Shenzhen 518116,China;[3]institute of Pediatrics,Shenzhen Children’s Hospital,Shenzhen 518038,China;[4]Respiratory Department,Shenzhen Children's Hospital,Shenzhen 518038,China;[5]Shenzhen Han’s Tech Limited Company,Shenzhen 518000,China;[6]Shenzhen Metasensing Tech Limited Company,Shenzhen 518000,China;[7]Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah 21589,Saudi Arabia;[8]Research Center for Advanced Materials Science(ROAMS),King Khalid University,Abha 61413,Saudi Arabia;[9]Department of Chemistry,College of Science,King Khalid University,Abha 61413,Saudi Arabia;[10]Zhongmin(Shenzhen)Intelligent Ecology Co.Ltd,Shenzhen 518055,China;[11]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China;[12]Department of Laboratory Medicine,Shenzhen Children's Hospital,Shenzhen 518038,China;[13]Optoelectronics Research Center,School of Science,Minzu University of China,Beijing 100081,China;[14]NHCKey Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;[15]Research Unit of Adaptive Evolution and Control of Emerging Viruses,Chinese Academy of Medical Sciences,Beijing 102206,China;[16]Institute of Pathogenic Organism,Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China高影响力机构 出　　处：《National Science Review》索引2022年第9卷第8期,共10页高影响力期刊 基　　金：supported by the Shenzhen Key Medical Discipline Construction Fund(SZXK032);the Guangdong Scientific and Technological Project(2019B1515120043,2020A151501612,2021A1515220109 and 2022B1515020093);the Science and Technology Innovation Commission of Shenzhen(KCXFZ20201221173413038);the Longhua District Science and Innovation Commission Project Grants of Shenzhen(JCYJ201904);the Innovation Team Project of the Department of Education of Guangdong Province(2018KCXTD026);supported by the Deanship of Scientific Research(DSR)at King Abdulaziz University,Jeddah(KEP-MSc-70-130-42);the funding from King Khalid University through the Research Center for Advanced Materials Science(RCAMS)(RCAMS/KKU/006/21);the Shenzhen Science and Technology Innovation Commission Key Project(JSGG20200225152648408);the Shenzhen Science and Technology Plan Project(JCYJ20200109105608771)。 摘　　要：The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. 关 键 词：surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant
相关文献

参考文献(41)

引证文献(20)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费