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Antioxidant and ACE inhibitory activities of peptides prepared from adzuki bean by semi-solid enzymatic hydrolysis

查看全文 作  者:Mengdi [1,3]Cao;Wenhui [1]Li;He [1]Li;Jian [1]Zhang;Yu [2]Liu;Xinqi [1]Liu 高影响力作者 机构地区:[1]National Soybean Processing Industry Technology Innovation Center,School of Food and Health,Beijing Technology and Business University,Beijing,100048,China;[2]Beijing Institute of Collaborative Innovation,Beijing,100094,China;[3]Chinese Academy of Inspection and Quarantine,Beijing,100123,China高影响力机构 出  处:《Food Bioscience》索引2022年第47卷第3期,共9页高影响力期刊 基  金:supported by the National Key Research and Development Program of China[2021YFC2101400]. 摘  要:In this study,adzuki bean peptides were prepared by semi-solid enzymatic hydrolysis(SEH)and their antioxidant and Angiotensin-I-converting enzyme(ACE)inhibitory activities and internal absorption were studied.By modifying different enzymatic hydrolysis times and enzyme additions,the optimal enzymatic hydrolysis condition for SEH could be obtained as adzuki bean powder:water=4:1(w/w),alcalase:neutrase=2:1(w/w),enzyme addition 3.75%(w/w),4 h enzymatic hydrolysis at 50℃ and pH=8.Within these hydrolysis conditions,the content of adzuki bean peptides was 11.37%,the OH−scavenging rate of 1 mg/mL peptides was 72.75%,the total antioxidant capacity was 0.88±0.02 mmol/L FeSO4,the ACE inhibition rate was 77.49%.In vivo absorption test proved that the peptides prepared by SEH were absorbed faster and exhibited higher antioxidant activity compared with liquid enzymatic hydrolysis.In conclusion,the process of SEH can improve not only the efficiency of hydrolysis,but also the antioxidant and ACE inhibitory activity as well as the internal absorption of adzuki bean peptides. 关 键 词:Adzuki bean peptides Semi-solid enzymolysis ANTIOXIDANT ACE inhibition ABSORPTION
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