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Engineered human spinal cord-like tissues with dorsal and ventral neuronal progenitors for spinal cord injury repair in rats and monkeys

查看全文 作　　者：Bai [1,2]Xu;Dingyang [3,4]Liu;Weiyuan [1]Liu;Ge [5]Long;Wenbin [1]Liu;Yayu [1]Wu;Xinghui [3,4]He;Yeyu [4,6]Shen;Peipei [7]Jiang;Man [1]Yin;Yongheng [1]Fan;He [8]Shen;Liyang [9]Shi;Qi [1]Zhang;Weiwei [1]Xue;Chen [1]Jin;Zhenni [1]Chen;Bing [1]Chen;Jiayin [1]Li;Yali [7]Hu;Xing [4]Li;Zhifeng [1]Xiao;Yannan [1]Zhao;Jianwu [1,8]Dai 高影响力作者 机构地区：[1]State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101,China;[2]Wuxi School of Medicine,Jiangnan University,Wuxi,214122,China;[3]Department of Neurosurgery,Xiangya Hospital of Central South University,Changsha,410008,China;[4]College of Life Science,Central South University,Changsha,410078,China;[5]Department of Anesthesia,The Third Xiangya Hospital of Central South University,Changsha,410013,China;[6]Department of Spine Surgery,Xiangya Hospital of Central South University,Changsha,410008,China;[7]Department of Obstetrics and Gynecology,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,210008,China;[8]Division of Nanobiomedicine,Suzhou Institute of Nano-Tech and Nano-Bionics,Chinese Academy of Sciences,Suzhou,215123,China;[9]College of Biology,Hunan University,Changsha,410012,China高影响力机构 出　　处：《Bioactive Materials》索引2023年第9期,共13页高影响力期刊 基　　金：supported by grants from the National Natural Science Foundation of China(No.81891002);the Strategic Priority Research Program of the Chinese Academy of Sciences(,XDA16040702 XDA16040704);Youth Innovation Promotion Association CAS(Grant No.Y202031);We also thanked the support from CAS Project for Young Scientists in Basic Research(Grant No.YSBR073);the Key Research and Development Program of Hunan Province(2021DK2003). 摘　　要：Transplanting human neural progenitor cells is a promising method of replenishing the lost neurons after spinal cord injury (SCI), but differentiating neural progenitor cells into the diverse types of mature functional spinal cord neurons in vivo is challenging. In this study, engineered human embryonic spinal cord-like tissues with dorsal and ventral neuronal characters (DV-SC) were generated by inducing human neural progenitor cells (hscNPCs) to differentiate into various types of dorsal and ventral neuronal cells on collagen scaffold in vitro. Transplantation of DV-SC into complete SCI models in rats and monkeys showed better therapeutic effects than undifferentiated hscNPCs, including pronounced cell survival and maturation. DV-SC formed a targeted connection with the host’s ascending and descending axons, partially restored interrupted neural circuits, and improved motor evoked potentials and the hindlimb function of animals with SCI. This suggests that the transplantation of pre-differentiated hscNPCs with spinal cord dorsal and ventral neuronal characteristics could be a promising strategy for SCI repair. 关 键 词：Spinal cord injury Neuronal progenitors Tissue engineering
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