The soybean GmPUB21-interacting protein GmDi19-5 responds to drought and salinity stresses via an ABA-dependent pathway

查看全文 作　　者：Yunhua [1]Yang;Rui [2]Ren;Adhimoolam [3]Karthikeyan;Jinlong [1]Yin;Tongtong [1]Jin;Fei [1]Fang;Han [1]Cai;Mengzhuo [1]Liu;Dagang [1]Wang;Haijian [1]Zhi;Kai [1]Li

机构地区：[1]National Center for Soybean Improvement,National Key Laboratory for Crop Genetics and Germplasm Enhancement,Key Laboratory of Biology and Genetic Improvement of Soybean Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;[2]College of Agronomy&Center for Crop Genome Engineering,Henan Agricultural University,Zhengzhou 450046,Henan,China;[3]Subtropical Horticulture Research Institute,Jeju National University,Jeju 63243,Republic of Korea

出　　处：《The Crop Journal》索引2023年第11卷第4期,共11页

基　　金：supported by the National Key Research and Development Program of China (2022YFF1001500);the Open Competition Project of Seed Industry Revitalization of Jiangsu Province (JBGS[2021]060);the Core Technology Development for Breeding Program of Jiangsu Province (JBGS-2021-014);China Agriculture Research System of MOF and MARA (CARS-04);the Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP);Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry (CIC-MCP)。 摘　　要：Drought-induced protein 19(Di19) is a Cys2/His2 zinc-finger protein that functions in plant growth and development and in tolerance to abiotic stresses.Gm PUB21,an E3 ubiquitin ligase,negatively regulates drought and salinity response in soybean.We identified potential interaction target proteins of Gm PUB21by yeast two-hybrid c DNA library screening,Gm Di19-5 as a candidate.Bimolecular fluorescence complementation and glutathionine-S-transferase pull-down assays confirmed the interaction between Gm Di19-5 and Gm PUB21.Gm Di19-5 was induced by Na Cl,drought,and abscisic acid(ABA) treatments.Gm Di19-5 was expressed in the cytoplasm and nucleus.Gm Di19-5 overexpression conferred hypersensitivity to drought and high salinity,whereas Gm Di19-5 silencing increased drought and salinity tolerance.Transcripts of ABA-and stress response-associated genes including Gm RAB18 and Gm DREB2A were downregulated in Gm Di19-5-overexpressing plants under drought and salinity stresses.ABA decreased the protein level of Gm Di19-5 in vivo,whereas Gm PUB21 increased the decrease of Gm Di19-5 after exogenous ABA application.The accumulation of Gm PUB21 was also inhibited by Gm Di19-5.We conclude that Gm PUB21 and Gm Di19-5 collaborate to regulate drought and salinity tolerance via an ABA-dependent pathway. 关键词：SOYBEAN Drought and salinity stresses GmDi19-5 GmPUB21 ABA-dependent pathway

相关文献

参考文献（71）
引证文献（2）
耦合文献（0）

参考文献(71)

引证文献(2)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

The soybean GmPUB21-interacting protein GmDi19-5 responds to drought and salinity stresses via an ABA-dependent pathway

参考文献(71)

引证文献(2)

用户提示