Over-expression of LPTS-Lin hepatocellular carcinoma cell line SMMC-7721 induces crisis

查看全文 作　　者：Cheng Liao Mu-Jun Zhao Jing Zhao Di Jia Hai Song Zai-Ping Li,State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《World Journal of Gastroenterology》索引2002年第8卷第6期,共3页

基　　金：a grant from National High Technology'863'Program of China No.2001AA221021;a grant from Special Funds for Major State Basic Research'973'of China No.001CB510205; a grant from the National Natural Sciences Foundation of China No.30170524. 摘　　要：AIM: To evaluate the function of the longer transcripts LPTS-Lin hepatocellular carcinoma cell line SMMC-7721.METHODS: SMMC-7721 cells were transfected with LPTSL expression construct and stably transfected cells were selected by G418. Multiple single clones formed and were checked for their phenotype. In the study of the effect on telomerase activity of LPTS-Lin vitro, GST-LPTS-L fusion protein was expressed in E.coli and purified by glutathioneagarose column. Telomeric repeat amplification protocol (TRAP) assays were performed to study the influence of telomerase activity in SMMC-7721 cells.RESULTS: Over-expression of LPTS-L induced SMMC-7721 cells into crisis. LPTS-L could inhibit the telomerase activity in SMMC-7721 cellsin vitro.CONCLUSION: LPTS-L is a potent telomeraseinhibitor. Over-expression of LPTS-L can induce hepatoma cells into crisis due to the reduction of telomerase activity. 关键词：肝细胞癌 SMMC-7721细胞系 LPTS-L基因表达过表达诱发危象

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Over-expression of LPTS-Lin hepatocellular carcinoma cell line SMMC-7721 induces crisis

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