Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process

查看全文 作　　者：MENG Xiangxian, WANG Kemin, TAN Weihong, LI Jun, TANG Zhiwen, GUO Qiuping, HUANG Shasheng & LI Du State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Biological Technology, College of Chemistry and Chemical Engi-neering, Hunan University, Changsha 410082, China;Correspondence should be addressed to Wang Kemin (e-mail: kmwang @hnu.net.cn)

出　　处：《Chinese Science Bulletin》索引2003年第48卷第23期,共4页

基　　金：supported by the National Key Basic Research Program(Grant No.2002CB513100);the Pre-Key Project of Basic Research of the Ministry of Sci.and Tec.of China(Grant No.2001-51);the Key Project of the National Natural Science Foundation of China(Grant No.20135010);the Overseas Youth Scholar Co-research Foundation of China(Grant No.20028506). 摘　　要：Ribozyme probe based on molecular beacon (MBR) for monitoring enzymatic cleavage process in real time is designed and studied. The approach relies on ri-bozyme substrates modified at the two arms, with a fluores-cent moiety attached to the end of one arm and a non-fluorescent quenching moiety attached to the end of the other arm. MBR is employed to directly convert the cleavage information into fluorescence signal in real time. Compared with traditional approach, this method provides a no-radiolabeling, sensitive and effective way to research on the ribozyme activity, enzymatic dynamic process and ri-bozyme function during gene therapy. The activity of the ribozyme against hepatitis C virus RNA (HCV-RNA) is stud-ied based on this assay. 关键词：酶探测器分裂过程动力学酶活性基因治疗肝炎

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Ribozyme probe based on molecular beacon for real time monitoring of enzymatic cleavage process

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