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| 1 | Effect of Discontinuous Administration of β -glucan and Glycyrrhizin on the Growth and Immunity of Pacific White Shrimp Litopenaeus Vannamei显示文摘 | Nan Bai Kangsen Mai Wenbing Zhang Hongming Ma Qinghui Ai Xiaojie Wang | 2010 | Feed & Livestock2010，3，3： | 20 |
| 2 | In Vitro Probiotic Effect of Pseudostellaria heterophylla Fibrous Roots on Feed Bacillus subtilis and Preliminary Study on Its Solid Fermentation显示文摘Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010;1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex;the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry. | Pan Huiqing Zhang Yanda Li Zhujin Zhao Qi | 2019 | Animal Husbandry and Feed Science2019，11，2： | 14 |
| 3 | Comparison of DPPH Scavenging Ability of Flavonoid and Polysaccharide from Dandelion ( Taraxacum mongolicum)显示文摘The paper was to study DPPH(1,1-diphenyl-2-trinitrobenzene hydrazine) scavenging ability of different concentrations of flavonoid and polysaccharide from dandelion. Flavonoids were extracted from dandelion using alcohol precipitation ultrasonic extraction method and polysaccharide was extracted using water ultrasonic extraction method. DPPH free radical scavenging ability of flavonoid and polysaccharide was determined using ultraviolet spectrophotometer method.The difference in DPPH free radical scavenging ability between flavonoids and polysaccharide was analyzed statistically. The results showed that DPPH scavenging ability was the best when the concentration of flavonoid was 1. 6 mg/m L; the scavenge rate was up to 96. 11% and IC50(median inhibitory concentration of inhibitors) was 0. 38 mg/m L. DPPH scavenging ability was the best when the concentration of polysaccharide was 1. 4 mg/m L; the scavenge rate was 89. 33%and IC50 was 0. 51 mg/m L. Statistical results showed that there were significant differences in DPPH free radical scavenging ability between dandelion flavonoid and polysaccharide( P < 0. 05). Flavonoid had significantly higher scavenging ability than polysaccharide( P < 0. 05). The study lays a theoretical foundation for further research on dandelion flavonoid,and provides a theoretical basis for development and utilization of dandelion resources. | Huang Dan Gu Ying | 2016 | Animal Husbandry and Feed Science2016，8，6： | 9 |
| 4 | Genetic Variation Analysis on the Whole Genomic Sequence of a H9N2 Subtype Avian Influenza Virus Isolate显示文摘[Objective] This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate(A/Chicken/Hebei/WD/98,abbreviated as WD98) by comparing with other reference strains.[Method] Eight complete genes were amplified by RT-PCR and sequenced.The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains.[Result] The whole genomic sequence of WD98 strain was 91.1%-95.8% homologous to that of seven reference strains tested.This isolate shared the highest homology(95.8%) to D/HK/Y280/97 and the lowest homology(91.1%) to C/Pak/2/99.The HA cleavage site of the WD98 strain was R-S-S-R↓G,and the 226th amino acid at receptor-binding site was Gln.[Conclusion] WD98 strain belongs to mildly pathogenic avian influenza virus and may not infect human.The genetic relationship is the closest between A/Chicken/Hebei/WD/98 and A/duck/HongKong/Y280/97,both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line.And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line. | YU Bo ZHANG Zhen-hua JIANG Bei-yu QIAN Ai-dong LI Lin JING Xiao-dong ZHANG Jian-wei | 2009 | Animal Husbandry and Feed Science2009，1，11： | 7 |
| 5 | Analysis on Nutrient Compositions and Nutritional Quality of Lota lota( Linnaeus) Muscle显示文摘In order to evaluate the nutritional quality of Lota lota(Linnaeus) muscle,the common nutrient components,amino acid composition and fatty acid composition in muscle of wild Lota lota(captured in Hailang reaches of Mudan River) were determined by routine methods. The results showed that the contents of moisture,crude protein,crude fat and ash were 81. 82%,15. 93%,0. 44% and 1. 06% in fresh muscle of Lota lota,respectively. Totally 17 amino acids,including seven kinds of essential amino acids(EAA),were detected in dry muscle of Lota lota(Linnaeus),accounting for 72. 43% of total dry muscle. The content of EAA was 29. 82%,accounting for 41. 17% of TAA,meeting the amino acid composition of high-quality protein standard required by Food and Agriculture Organization /Word Health Organization(FAO /WHO). There were four delicious amino acids(DAA) in dry muscle,accounting for 28. 34% of total dry weight.The percentage of DAA to TAA was 39. 13% in dry muscle. The contents of C20:5n- 3(EPA) and C22:6n- 3(DHA) in dry muscle were 8. 65% and 20. 32%respectively,which were significantly higher than that of the artificially cultured fish species,such as Oxyeleotris marmorata(EPA:2. 24%,DHA:4. 74%),Spinibarbus sinensis(EPA:0. 87%,DHA:3. 08%) and Glossogobius giuris(EPA: 3. 94%,DHA: 8. 10%). Based on the amino acids score(AAS) and chemical score(CS),the first limited amino acid in Lota lota(Linnaeus) was sulfur-containing amino acid(methionine + cysteine). The essential amino acids index(EAAI) of Lota lota(Linnaeus) was 62.58. It is concluded that Lota lota(Linnaeus) is abundant in amino acids and high-unsaturated fatty acids,and rational in composition of amino acids. Thus,Lota lota(Linnaeus) is a high quality freshwater fish with high nutritional value and health value,possessing good exploitation and utilization prospects. | Xu Gefeng Wang Yuyu Bai Qingli Li Xiaolong Jia Zhonghe | 2014 | Animal Husbandry and Feed Science2014，6，4： | 5 |
| 6 | Identification Effects of Pedometer on Estrus of Holstein Cows during Peak Lactation Period显示文摘[Objective]The paper was to discuss identification effects of pedometer on estrus of Holstein cows during peak lactation period. [Method]The estrus of Holstein cows during peak lactation period were identified by manual observation and pedometer monitoring. [Result]Compared with manual observation,the detection rate of estrus in Holstein cows using pedometer monitoring was increased by 24. 01%,which reduced the labor cost and enhanced accuracy rate. [Conclusion]The research could provide reliable basis for estrus identification of cows. | Jiang Xiaoxin Deng Shuangyi Liu Wei Wei Xingyuan Ai Li | 2014 | Animal Husbandry and Feed Science2014，6，2： | 5 |
| 7 | Sources and Accumulation Risk of Heavy Metal in Major Animal Manure显示文摘Feed and corresponding manure samples were collected from 27 scale pig farms and 7 scale chicken farms in Jiaxing City,Zhejiang Province,and their heavy metal contents were analyzed. The weighted average values of Cr,Cu,Zn,Pb and As in pig feed were 4. 6,123. 4,370. 7,0. 69 and 6. 9 mg/kg,respectively. However,referred to Safe Use Specification of Feed Additives and MOA Announcement No. 1224,the exceeding standards rates of Cr,Cu,Zn,Pb and As in pig feed were 5. 36%,62. 5%,19. 64%,3. 57% and 37. 5%,respectively. The sequences of heavy metal content in pig feed successively were piglet > growing pig > big pig > sow,but the exceeding standard rates of heavy metal in feed were big pig > sow > growing pig > piglet,and the exceeding standard rate of big pig feed was more than 95%. The exceeding standard rates of Cu,Zn and As in chicken feed were 46. 7%,11. 1% and 33. 3% respectively. Based on Organic Fertilizer( NY525-2012) and Sludge Agricultural Pollution Control Standard( GB4284-84),Cu,Zn and As in pig manure and chicken manure exceeded the standard; the exceeding standard rates of pig manure were 71. 9%,78. 9% and 42. 1%,while that of chicken manure were 12. 5%,25. 0% and 44. 4%,respectively. The exceeding standard rates of Cu and Zn in piglet manure reached 100%. There were very significant positive correlations in Cu,Zn and As between pig feed and corresponding manure,or Hg,Cu and As between chicken feed and corresponding manure,and the correlation coefficients r^2 were greater than 0. 8. Besides,the correlation coefficient r^2 of Pb between pig feed and corresponding manure was above 0. 6. Continuous and high intensity of livestock manure application significantly promoted the accumulation of heavy metal in farmland soil,but the effects on heavy metal accumulation in rice was not significant. | Shi Yanping Huang Jinfa Ni Xiongwei Xie Jianping Ren Jiajia | 2016 | Animal Husbandry and Feed Science2016，8，6： | 4 |
| 8 | Sow Reproductive Stress and Its Syndrome显示文摘In this paper,we put forward 'Reproductive Stress' hypothesis,and the main viewpoints are as below.The special reproductive tasks such as estrus,pregnancy,childbirth and lactation themselves are stressors which cause violent stress,and this kind of stress was called reproductive stress.Fetus is also a special stressor for female.The corticotropin releasing hormone (CRH) and fetal glucocorticoid (GC) secreted by fetus and placenta also cause stress,and this kind of stress was named female passive stress.Fetus develops in uterus,and the stress caused by uterine tension and intrauterine environment was named fetal intrauterine stress.Humans and animals need stress mechanisms to help themselves accomplish reproductive tasks,e.g.,involvement of CRH,adrenocorticotropic hormone (ACTH) and epinephrine.Therefore,childbirth may be originally motivated by the fetal intrauterine stress.Under the conditions of not enough nutrition or heavier reproductive tasks,females have to mobilize body reservoir to assure reproducing that aggravates reproductive stress.Moreover,reproductive stress is a long lasting experience for female,which must cause changes in endocrine secretion (e.g.,constant increasing of CRH and GC).It thereby weakens the female's immune functions,causes body damages and makes them more susceptible to diseases.The main clinic symptoms include leg and foot weakness syndrome,anoestrus,subinvolution of uterus and endometritis.We summarized the causes,main clinical symptoms and pathogenesis of reproductive stress and provided applicable measures to prevent sows from reproductive stress. | WEN Li-xin YUAN Hui XIE Lan | 2009 | Animal Husbandry and Feed Science2009，1，8： | 4 |
| 9 | Optimization on Production Key Factors of Microbial Fermented Feed and Analysis on Composition Variation During Fermentation显示文摘[Objective] In order to study the production key factors of microbial fermented feed and the composition variation during fermentation. [Methods]Water,temperature,energy and protein were selected to study the effects on microbial fermented feed. And the fermentation conditions were optimized. Changes on nutrient composition during fermentation were determined and analyzed. [Result] Test results showed that feed formula with high sugar content,low protein,34%-36% water content,high fermentation temperature was more conducive to the production and actual production needs of fermentation strains. During the fermentation,strains showed interactions,test tended to be completed at the 144^(th)h after the fermentation,and number of lactic acid bacteria reached the peak of 1. 86 × 10~9 strain/g,contents of lactic acid could even reach 0. 89%. As time prolonged,contents of total energy,fiber and isothiocyanate gradually reduced,while contents of water and protein increased slightly. Contents of vitamin were stable,which in an order of V_B> V_E> V_K> V_A> V_D. Composition of amino acids showed an better change,contents of glutamic acid and proline decreased slowly,while contents of glycine and phenylalanine increased slowly. [Conclusion]High quality microbial fermentation feed could improve the palatability and safety of feed and keep the intestinal balance of livestock and poultry,which had broad application prospect. | Lin Biaosheng Luo Jian Li Yiming He Yuqin Luo Maochun Yang Xiaoyan | 2017 | Animal Husbandry and Feed Science2017，9，3： | 4 |
| 10 | Research Progress of Active Ingredients and Pharmacological Action of Honeysuckle显示文摘Honeysuckle,the dry flower bud or with open flower of Lonicera japonica in caprifoliaceae,has been touted as a cure-all for heat-clearing and detoxifying effect.Honeysuckle contains abundant components such as volatile oil,flavonoids and organic acids,playing the role of antibiosis,antivirus,antiphlogosis,immunopotentiation and antioxidation.The active ingredients and pharmacological action of honeysuckle are reviewed in the paper,so as to lay the foundation for further application of honeysuckle in veterinary clinic. | Zhang Zhimei Guo Shijin Fu Shijun Xu Qianqian Yang Limei Zhang Ying Wang Yanping Ma Li Wang Jianjun Shen Zhiqiang | 2014 | Animal Husbandry and Feed Science2014，6，1： | 4 |
| 11 | Ultrastructure Observation of Cysticercus Pisiformis and Its Function Analysis显示文摘[Objective]The aim of the study was to observe the ultrastructure of Cysticercus pisiformis under different state. [Method] The Cysticercus pisiformis were obtained in the procedure of slaughter inspection form meat-packing plant. The Cysticercus pisiformis was taken out by cutting cyst directly, or cultured with physiologic saline containing 10% bile until evagination. Then it was observed and recorded by scanning electron microscopy. [Result]Observed from top of scolex of Cysticercus pisiformis locate in cyst, the rostellum looked like an umbrella. The cutaneous muscular columns with hamula covered the front end of scolex. Viewed from the side, a row of antlers-liking hamula adhered to the scolex. Four suckers were looked like more width and body segments less fime, in which there were lots of wrinkles in their surface respectively. The Cysticercus pisiformis cultured was divided obviously into scolex, neck, body segment and zone of cyst. When cutaneous muscular column of rostellum contracted, the antler-liking hamula extended to periphery that the ring-liking contraction appeared in neck and the lengthways contraction in body segments. [Conclusion] The Cysticercus pisiformis under different state has distinctly dissimilar ultrastructure. | PAN Yao-qian ZHAO Zhen-sheng CHEN Jin-shan YIN Mei TANG Hai-rong LI Rui-zheng LI Hui-zheng LIU Zhi-ke ZHANG Li-jie LIU Xing-you | 2012 | Animal Husbandry and Feed Science2012，4，4： | 4 |
| 12 | Detection of Lactobacillus acidophilus in Fermented Material by Real-time Fluorescent Quantitative PCR显示文摘The species distinctive PCR primer of Lactobacillus acidophilus( L. acidophilus) was designed according to 16 S rRNA gene sequences of common Lactobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template,and L. acidophilus in fermented material was conducted the quantitative determination by real-time quantitative PCR( RT-PCR). Analysis on RT-PCR results shown that contents of L. acidophilus in the test sample reached 1. 5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test,indicating that the established RT-PCR method could be applied to the detection of L. acidophilus in fermented material. | Guo Zihao Fang Hua Xia Zhisheng Zhu Xiaoshi Sun Zhongchao Yu Hanli Xia Jiaji | 2016 | Animal Husbandry and Feed Science2016，8，1： | 4 |
| 13 | Effects on Growth Performance and Feed Digestibility of Hainan Black Pigs by Adding Lysine in Low Protein Diet显示文摘[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low protein diet.[Method] A total of 36 Hainan black pigs with the body weight of(46.34 ±3.06) kg were randomly divided into three groups:control group(protein level 15%,lysine level 0.83%),experimental group I(protein level 13%,0.83%),and experimental group II(protein level 13%,lysine level 1.03),each group had three replicates of four pigs.The trial lasted 35 d.[Result] The growth performance of Hainan black pig in group II had no significant difference with that in control group(P>0.05).The apparent digestibility of crude protein and phosphorus of Hainan black pigs in Group II increased significantly(P<0.05),which were increased by 10.08% and 11.85% respectively compared to the control group;the apparent digestibility of calcium was increased,but the difference was not significant(P>0.05).[Conclusion] It will not reduce the growth performance of Hainan black pig when the protein level of diet is reduced by 2% and the lysine level is increased to 1.03%.It is recommended that the diet can be used in the production of Hainan black pig. | Wei Limin Liu Quanwei Sun Ruiping Zheng Xinli Wang Feng Tan Shuyi Huang Lili Xing Manping | 2018 | Animal Husbandry and Feed Science2018，10，2： | 4 |
| 14 | Effect of Pelleted Total Mixed Ration on Production Performance and Serum Biochemical Index of Growing Hainan Black Goats显示文摘[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat. | Liu Quanwei Wang Feng Wei Limin Huang Lili Sun Ruiping Xing Manping Zheng Xinli | 2019 | Animal Husbandry and Feed Science2019，11，1： | 4 |
| 15 | Nutritional Quality of Mixed Silage of Lablab purpureus and Sweet Sorghum显示文摘[Objective] The paper was to evaluate the fermentation quality of mixed silage of Lablab purpureus and sweet sorghum,and to find out the appropriate mixing ratio.[Method]L.purpureus were mixed with sweet sorghum at different proportions,to identify the sensory character and quality of silage.[Result]The nutrient content of mixed silage of L.purpureus and sweet sorghum at different proportions decreased significantly at 30-60 d,while no significant changes were observed after 60 d.Mixed silage of L.purpureus and sweet sorghum had the best effort at the proportion of 3∶7; followed by the proportion of 5∶5.These two proportions significantly improved dry matter( DM) content and effectively alleviated the crude protein( CP) loss of raw materials; significantly improved the contents of crude fiber and crude ash; and significantly reduce ammonia nitrogen/total nitrogen( AT/TN).[Conclusion]From the perspective of silage quality,the appropriate mixing ratio of L.purpureus and sweet sorghum is 3∶7 or 5∶5. | Liu Hailin Deng Huifen Xu Huaqin Sun Ao | 2017 | Animal Husbandry and Feed Science2017，9，6： | 4 |
| 16 | Effects of Microencapsulated Compound Acidifier on Acidity and Development of Gastrointestinal Tract in Weaning Piglets显示文摘The effects of different types of compound acidifiers (encapsulated and non-encapsulated) in maize-soybean basic diets on acidity and development of the gastrointestinal tract in weaning piglets were investigated in this study. 64 28-day-old weaned Landrace×Yorkshire hybrid piglets with average weight of (7.00±0.10) kg were selected and grouped into four treatments with four pigs (half boars and half sows) in each repeat of four repeats in each treatment based on single-factor test design principles, and the pre-test period was 3 days but the test period was 35 days. The results showed that compared with acid-free diet group, encapsulated compound acidifier could reduce pH of stomach and intestinal in weaning piglets significantly (P<0.01), while Test group 1 could also increase the relative weight of stomach and intestinal in piglets significantly (P<0.05). Compared with non-encapsulated acidifier, encapsulated compound acidifier could greatly decrease pH in jejunum and ileum of weaning piglets (P<0.05) or relative weight of stomach in piglets (P<0.05), while Test group 1 could also enhance the relative weight of intestinal in piglets significantly (P<0.01). In addition, encapsulated compound acidifiers significantly increased the ratio between the villus height and crypt depth of jejunum (P<0.01). Accordingly, the microencapsulated compound acidifier in diets of weaning piglets can promote the development of gastrointestinal tracts for piglets by reducing pH of gastrointestinal tracts. | JIA Gang YAN Jia-you WANG Kang-ning | 2009 | Animal Husbandry and Feed Science2009，1，6： | 4 |
| 17 | The Effects of Compound Chinese Medicine Immune Additives on Growth Performance and Antioxidant Capacity of Acipenser schrenckii显示文摘[Objective] The aim was to explore the effects of compound Chinese medicine on growth and antioxidant capacity of Acipenser schrenckii,and to provide a foundation for the application of traditional Chinese medicine as immune additives in sturgeon aquaculture production.[Methods] Three kinds of compound Chinese medicines(respectively called A,B and C) were added into basic material feed as feed additives,continuous fed 1+ age Acipenser schrenckii for 35 d,then weight gain rate was determined and superoxide dismutase(SOD) activity,malondialdehyde(MDA) content,total antioxidant capacity(T-AOC) and glutathione peroxidase(GSH-PX) activity in serum and liver were detected.[Results] Chinese medicine formulae could increase the superoxide dismutase(SOD) activity,total antioxidant capacity(T-AOC) and glutathione peroxidase(GSH-PX) in serum and liver of sturgeon.Especially SOD enzyme activity of serum in the three formulae groups was significantly higher(P<0.05) than control group,SOD enzyme activities of fish liver in group A and C were significantly higher(P<0.05) than control group;T-AOC activities of serum and liver in the three formulae groups were both incressed,but had no significant differences with that of the control group;only GSH-PX activities of fish serum in group B and C had significant differences(P<0.05) with that of control group,GSH-PX activities of liver in the three formulae groups had no significant differences with that of control group;and traditional Chinese medicine could significantly reduce malondialdehyde(MDA) content of sturgeon serum,MDA contents of serum and liver in three formulae groups had significant differences(P<0.05) with that of control group.[Conclusion] The selected three kinds of compound Chinese medicine additives had different degree of ability to improve sturgeon antioxidant function. | LIU Xun WANG Di LU Tong-yan LI Shao-wu LIU Hong-bai | 2012 | Animal Husbandry and Feed Science2012，4，5： | 4 |
| 18 | Effects of Shuanghuanglian Propolis Oral Liquid on Growth Performance,Slaughter Performance and Meat Quality of Broilers显示文摘[Objective] The paper was to investigate the effects of adding Shuanghuanglian propolis oral liquid in drinking water on growth performance,slaughter performance and meat quality of broilers. [Method]Ninety one-day-old broilers were randomly divided into three groups( group Ⅰ,group Ⅱ and group Ⅲ) : group Ⅰ was the control group,and groups Ⅱ and Ⅲ were added 0. 5 and 1. 0 mL / L Shuanghuanglian propolis oral liquid in drinking water,respectively.[Result] Compared with broilers in group I,the average daily feed intakes of broilers in group Ⅱ and group Ⅲ were increased by 0. 86%( P > 0. 05) and 1. 10%( P > 0. 05),respectively; the average daily gains were increased by 2. 33%( P < 0. 05) and 4. 43%( P < 0. 05),respectively; the feed gain ratios were decreased by 1. 05%( P >0. 05) and 2. 63%( P <0. 05),respectively; the slaughter rates were increased by 0. 94%( P >0. 05) and 1. 70%( P <0. 05),respectively; the eviscerated rates were increased by 0. 52%( P > 0. 05) and 1. 15%( P > 0. 05),respectively; the semi-eviscerated rates were increased by 1. 51%( P < 0. 05) and 2.23%( P < 0. 05),respectively; the breast muscle rates were increased by 3. 77%( P < 0. 05) and 5. 51%( P < 0. 05),respectively; the leg muscle rates were increased by 1. 96%( P > 0. 05) and 4. 09%( P < 0. 05),respectively; the lightness( L*) were decreased by 0. 96%( P > 0. 05) and 1. 47%( P > 0. 05),respectively; the redness( a*) were increased by 1. 24%( P < 0. 05) and 1. 86%( P < 0. 05),respectively; the yellowness( b*) were decreased by 0. 44%( P >0. 05) and 1. 03%( P <0. 05),respectively; the drip losses were decreased by 1. 22%( P <0. 05) and 1. 56%( P <0. 05),respectively; the cooking losses were decreased by 1. 76%( P > 0. 05) and 2. 89%( P < 0. 05),respectively. [Conclusion]Adding 0. 5 and 1. 0 mL / L Shuanghuanglian propolis oral liquid in drinking water could improve growth performance,slaughter performance,meat quality of broilers,and the dose of 1. 0 mL / L is recommended in clinical application. | Ma Li Yang Limei Shen Zhiqiang Wang Yanping Xu Qianqian Zhang Ying Guo Shijin | 2016 | Animal Husbandry and Feed Science2016，8，4： | 4 |
| 19 | Protective Effect of Bacillus subtilis Peptidoglycan (PG) on β-conglycinin-induced Intestinal Epithelial Cells Damage of Juvenile Carp (Cyprinus carpio)显示文摘[Objective] The paper was to investigate the protective effects of different concentrations of Bacillus subtilis peptidoglycan(PG) on β-conglycinin-induced inflammatory injury in intestinal epithelial cells of juvenile carp(Cyprinus carpio).[Method] In 24-cell microplates, the intestinal epithelial cells(IECs) of juvenile carp were primarily cultured for 72 h at 26°C and 6% CO2, and then the IECs were randomly divided into6 groups with 4 replicates per group. One of the six groups was set as negative control group, and the other groups were all supplemented with 1.0 mg/mL β-conglycinin in culture medium to establish inflammatory injury. At 24 h post induction, the culture media were changed into B. subtilis PG culture media with the concentrations of 0(positive control group), 0.15, 0.30, 0.45 and 0.60 mg/mL, respectively. The samples were collected to measure the antioxidant and anti-inflammatory indices at 12, 24 and 36 h post culture.[Result]β-conglycinin exposure significantly decreased the activity of ASA, AHR, SOD, CAT, GPx, and increased the PC content and the mRNA expression of inflammatory cytokines(IL-1β, IL-8, TNF-α1,IL-10 and TGF-β). At 12, 24 and 36 h post PG treatment, the activities of ASA, AHR, SOD, CAT, GPx and the content of PC in cells decreased in a dose-dependent manner;the mRNA levels of IL-1β, IL-8 and TNF-α1 were down-regulated and those of IL-10 and TGF-β were up-regulated.[Conclusion] Different concentrations of B. subtilis PG could protect IECs oxidative damage induced by β-conglycinin and improve the antioxidant capacity of IECs. High concentration of PG could improve the anti-inflammatory ability of IECs by inhibiting inflammatory factors and promoting the gene expression of anti-inflammatory cytokines. | Zhang Tiantian Yin Haicheng Huang Wei | 2019 | Animal Husbandry and Feed Science2019，11，2： | 3 |
| 20 | Isolation and Identification of High-Quality Lactic Acid Bacteria in Forage Corn显示文摘[Objective] To screen suitable lactic acid bacterium strains from forage corn which can be used as silage additives.[Method] The lactic acid bacterium strains were isolated by inoculation on MRS solid medium containing calcium carbonate,and they were preliminarily identified through morphological,physiological and biochemical experiments.The acid production efficiency was determined.Twelve strains having strong acid-production ability were selected,and their salt tolerance and acid tolerance were detected.The sequences of their 16 S rDNA were also analyzed.[Result] A total of 44 lactic acid bacterium strains were isolated from the forage corn.As evidenced by the physiological and biochemical experiments,the twelve strains having strong acid-production ability belonged to Leuconostoc,Lactobacillus and Enterococcus,respectively,and they had strong salt tolerance and acid tolerance.According to the sequences of 16 S rDNA,A4,B9,B11,B12 and B14 were Lactobacillus plantarum;A1,A2,A7,A11 and B8 were Leuconostoc mesenteroides dextran subspecies;and A8 and A9 were Enterococcus hirae.[Conclusion] The lactic acid bacterium strains with strong acid-production ability isolated from forage corn can be developed into silage additives. | Mairemunisa.Aimaier Abudukeyoumu.Maimaiti Wusiman.Yimiti | 2011 | Animal Husbandry and Feed Science2011，3，1： | 3 |
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