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| 1 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 2 | Effect of Combined Heavy Metal Pollution on Nitrogen Mineralization Potential,Urease and Phosphatase Activities in a Typic Udic Ferrisol显示文摘INTRODUCTIONAnthropogenicactivitiessuchasmining,smelting,electroplating,ore-refiningandlanddisposalofwasteshaveledtoanincreajseofheavymetalcolltelltsinthesoil(Chen,1996).Heavymetalaccumulationinthesoilimpactsanumberofsoilpropertiesthatmightbethepoten... | ZHENG CHUNRONG TU CONG and CHEN HUAIMAN(Laboratory of Material Cycling in Pedosphere, Institute of Soil Science, the Chinese Academy of Sciences,Nanjing 210008 (China))(Received April 20, 1999 revised June 2, 1999) | 1999 | Pedosphere1999，9，3： | 13 |
| 3 | NumericalmodelforsolvingBousiinesq-typeequations:comparisonandvalidation显示文摘NumericalmodelforsolvingBousiinesq-typeequations:comparisonandvalidationZouShiliandXuBenhe(ReceivedMay20,1997;acceptedAugust1... | Zou Shili and Xu Benhe (Received May 20, 1997 accepted August 13, 1997) | 1998 | Acta Oceanologica Sinica1998，17，3： | 11 |
| 4 | InteractionoftheKuroshiowaterandshelfwaterintheTsushimaCurrentregioninsummer显示文摘InteractionoftheKuroshiowaterandshelfwaterintheTsushimaCurrentregioninsummerGuoBinghuo,1Heung-JaeLie2andJaeHakLee2(ReceivedSe... | Guo Binghuo Heung-Jae Lie and Jae Hak Lee2( Received September 20, 1997 accepted October 25, 1997) | 1998 | Acta Oceanologica Sinica1998，17，3： | 6 |
| 5 | NEW IDEAS AND METHODS ON GEOMECHANICAL SAFETY ANALYSIS OF TOXIC AND RADIOACTIVE WASTE REPOSITORIES IN SALT MINE显示文摘In view of the need for geomechanical safety analysis of repositories in salt rock, failure criteria,creep rupture criteria,material models,pillar design methods and criteria for the assessment of barrier efficiency as well as investigations of the interaction between hydraulics and mechanics for the case of uncontrolled flooded repositories are necessary. The introduction of damage mechanics and of the Hou/Lux material model including damages into geomechanical safety analysis of repositories in salt rock can reduce some previous deficits in knowledge and modelling. This article will be as a part of geotechnical assessment to introduce the Hou/Lux material model,a new concept of hydro-mechanical coupling and a pillar design method as well as criteria for the assessment of efficiency of geological barriers. | Hou Zhengmeng1 Wu Wen2 (1Institure of Disposal Technology and Geomechanics,Technical University of Clausthal,Erzstrasse 20,38678 Clausthal-Zellerfeld,Germany) (2Key Laboratory of Rock and Soil Mechanics,Institute of Rock and Soil Mechanics,The Chinese Academy of Sciences, Wuhan 430071 China) | 2002 | 岩石力学与工程学报2002，21，11： | 2 |
| 6 | At the crossroad of conception and infection: initiatives for immunoglobulin-based contraceptive R & D显示文摘Better understanding of the immunological inechanisms implying the insemination and the infertility of some menand women is needed and crucial to the developnlent of an effective imlntmocona'aceptive method. To provide goodprotection against conception or infection, and avoid any possible and unexpected complications which immunocontra-cepfive 'vaccine' my arise of, it seems the right time for scientists to create a virtually new thinking for this extremelyurgent and important issue. This conceptual article describes our original thoughts of the future development of im-munocontracepdves, preferably, based on immunoglobulins rather than vaccines, against human sperm specific antigensand seminal plasma immtmosuppressive factors. Its general correctness, advantages and feasibility for fertility regula-tion and prevention of infection are discussed. | Nian-Qing LU Yi-Fei WANG Yu-Feng HUANG Xing-Hai WANG Jiangsu Family Planning Research Institute, Nanjing 210029, China Department of Reproductive Health and Research, WHO, 20 Avenue Appia, CH 1211 Geneva, Switzerland Nanjing General Hospital, Nanjing 210002, China | 1999 | Asian Journal of Andrology1999，1，3： | 2 |
| 7 | Types of Organic Fades and Source Rock Assessment of the Coal-Measure Mudstone in the Turpan-Hami Basin显示文摘This paper discusses the enviromental characteristics of carbonaceous mudstone and mudstone (coal-measure mudstone in short) of the Early and Middle Jurassic in the Turpan-Hami basin, which were formed in swamps. Through the organic facies study of the coal-measure mudstone in this area, the authors clarify that the flowing-water swamp is the most advanced organic facies belt. Furthermore, according to the practical materials of coal-measure mudstone in the area and with the evaluation criteria of lacustrine mudstone in the past, the authors have established the integrated symbol systems from the abundance of organic matter and the type of organic matter, which can be used in the source rock evaluation of the coal-measure mudstone. | ZHAO Changyi, DU Meili, SHAO Longyi, CHEN Jianping,CHENG Kerning and HE ZhonghuaResearch Institute of Petroleum Exploration and Development,China National Petroleum Corporation, 20 Xueyuan Rd., Beijing 100083Beijing Graduate School of China University of Mining and Technology,11 Xueyuan Rd., Beijing 100083 | 1998 | Acta Geologica Sinica(English Edition)1998，72，2： | 2 |
| 8 | Weld Models Incorporating the HAZ Phase Transformation Effects, Comparison between Experimental and Numerical Results显示文摘Numerical simulations of a representative test of welding process are presented in this paper. A French vessel steel, which involves metallurgical phase transformations in solid state is considered in this work. The aim is to validate the thermal-metallurgical-mechanical models taking into account the metallurgical transformations in the finite element codes Sysweld (Framasoft) and Code Aster (EDF). The test is performed on a thin disc submitted to a thermal cycle loading by means of a CO2 laser beam, which leads to metallurgical phase transformations. The thermal, metallurgical and mechanical numerical results have been compared to the experimental results (temperatures, sizes of transformed zones, displacements and residual stresses and strains). The main objective of the numerical analysis is to have some results which enable to give some indications on the ability of the numerical codes to describe the observed phenomena. For that, it is necessary to simulate accurately the thermo-metallurgical history. The comparison of experimental results with the numerical ones leads to some interesting orientations related to the capacities of the considered models to describe the observed phenomena. | V. VINCENT(1-2), J.F. JULLIEN(1), F. FOUQUET(1) and V. CANO(3) (1) INSA de Lyon, 20 Avenue Albert Einstein, 69621 Villeurbanne Cedex, France (2) FRAMATOME, 92084 Paris La defense Cedex, France (3) EDF-DER, 1 Avenue du General de Gaulle 92141 Clamart Cedex | 2000 | Journal of Shanghai Jiaotong university(Science)2000，5，1： | 2 |
| 9 | 显示文摘 | 徐光.? 关于新时期电力政工人员素质的思考(J)华章. 2013 (20) | 2013 | 华章2013，，20： | 1 |
| 10 | 显示文摘 | 罗哲平.? 地理教学融入社会主义核心价值观教育的探讨(J)华章. 2014 (20) | 2014 | 华章2014，，20： | 1 |
| 11 | Thestenocleidomastoidtumorofinfancy显示文摘 | 〔20〕TomLWC HandlerSD WetmoreRF etal | 1987 | JPediatrOtorhinolaryngol1987，13，： | 1 |
| 12 | Observations on breeding behavior of the white-eared night heron (Gorsachius magnifwus ) in northern Guangdong, China显示文摘 | Gao JY Wu ZL Su DD 20!3 | | Chinese Birds0，4，3： | 1 |
| 13 | Effects of arsenic on DNA synthesis in human lymphocytse显示文摘 | 20,Meng ZQ | 1993 | Arch Environ Contam Toxicol1993，25，： | 1 |
| 14 | E6-associated is required for hunman papillomavius type o cause cervical cancer in mice显示文摘 | SHAI A protein 16 E6t Res 20 PITOT H C LAMBERT P F | | Cancer 100，70，12： | 1 |
| 15 | Effect d dietary lipids on myocardial norepinephrine content and field stimilation-mediated release of norepinephrine from perfused neonatal and adult rat hearts显示文摘 | Semafuko WE Rutledge (20 Dixon WR | 1987 | J Cardiovasc Pharmacol1987，10，1： | 1 |
| 16 | Insertion of m ini2IS605 and deletionof adjacent se2quences in the nitro reductase(rdxA)gene causemetro-nidazole resistance in Helicobacter py lorlNCTCl 1637显示文摘 | Debets 20 ssenkopp YJ Po tR G vanW esterlooD J | 1999 | Antimicrob A gents Chemother1999，43，11： | 1 |
| 17 | Highly efficient rectangular pulse e- 显示文摘 | LI X ZHANG S ZHANG H mission in a mode-locked fiber laser IEEE 2014 26(20): 2082-2085 | 2014 | Photonics Technology Letters2014，，： | 1 |
| 18 | A k2A-positive Klebsiella pneumoniae causes liver and brain abscess in a Saint Kitt's man显示文摘 | Virulence and Transferability of Novel Klebsiella pneumoniae Microb Drug Resist 2014 20 Doud MS Grimes-Zeppegno R Molina E | 2009 | Int J Med Sci2009，6，6： | 1 |
| 19 | CAST ( Chinese Acute Stroke Trial ) Collaborative Group 显示文摘 | CAST:randomised placebo-controlled trial of early aspirin use in 20 000 patients with acute ischacmic stroke | 1997 | Lancet1997，349，9066： | 1 |
| 20 | 显示文摘 | 秦仰平.? 网络环境下大学生思想政治教育研究(J)华章. 2012 (20) | 2012 | 华章2012，，20： | 1 |
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