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    题名 作者 年代 出处 被引量
1稻瘟病菌株CH63和TH16杂交组合的遗传图谱构建及无毒基因定位显示文摘测定了CH63和TH16杂交后代(F1 代子囊孢子群体)在36个水稻品种上的致病性,依据F1 代子囊孢子群体在不同品种上的无毒性/毒性表型和SSR、SCAR和RAPD等分子标记的多态性位点共分离构建了稻瘟病菌的遗传图谱。图谱共有151个标记位点,7个连锁群,全长1038.4 cM。由杂交后代在不同品种上的无毒性/毒性分离鉴定出CH63菌株持有多个无毒基因,在该遗传图谱中初步定位了Avr2 K59、Avr C105 TTP1、Avr Zh156、Avr Xuan1641、Avr Xuan6392 和Avr C103 TTP 6个无毒基因,分别定位在第1、第4和第7染色体上。王艳丽 Claudia KAYE Amandine BORDAT Henri ADREIT Jo■lle MILLAZZO 郑小波 沈瑛 Didier THARREAU 2005中国水稻科学2005,19,2:9
2Geographic Distribution of Mating Types in Magnaporthe grisea and the Relationship Between Fertile Isolates in China显示文摘377 isolates of Magnaporthe grisea were collected from 17 provinces in China and their geographic distribution of mating types and their fertility was tested with four standard isolates, KA3 and TH12 (Mat1.1) and Guy11 and TH16 (Mat1.2) provided by CIRAD. 73 fertile isolates were tested with SCAR markers of 13 pairs of primers. Preliminary results showed that the geographic distribution of M. grisea existed among isolates collected from the same location as well as different locations and the genetic relationship between fertile isolates of the fungus in China. The existence of sexual reproduction of M. grisea was explored in the field as well.SHEN Ying, Notteghem Jean Loup., Milazzo Joelle., YUAN Xiao-ping, Adreit Henry, ZHAO Xin-hua, WANG Yan-li and Tharreau Didier.(China National Rice Research Institute, Hangzhou 310006, P. R. China National Institute of Agronomic Research ,Unit of Training and Biological Research , Ecology and Plant Pathology, 2 Place Viala , 34062 Montpellier , France International Center for collaboration in Agronomic Research and Development,TA 73 /09 , 34398 Montpellier , Cedex 05 , France) 2002Agricultural Sciences in China2002,1,6:5
3稻瘟菌株CH63和TH16杂交后代毒性变异及其无毒基因组成显示文摘报道了两个稻瘟菌株CH63和TH16的杂交后代在36个水稻品种上的致病性分离、遗传分析以及无毒基因组成的分析结果。78个子囊孢子菌株中呈现显著的致病性分离,共出现71种致病类型,在不同品种上分别出现1∶1、1∶3、3∶1、1∶7、1∶15和15∶1等的无毒性/毒性分离,表明供试菌株对不同品种的无毒性/毒性是一个或多个基因控制的。遗传学分析结果表明,亲本菌株对不同品种的无毒性/毒性控制存在多种类型。菌株CH63对品种K59(Pi-t)、中98-18、特特普、T641、C102TTP(Pi-4)、C101TTP6、ENo.11、农虎6号、C101PKT(Pi-4a)、C105TTP-1(Pi-4)、中156和中98-19分别持有1个无毒基因;对品种1461选、6392选、麻谷泰引1号、Lemont、宫崎7号、Vandana、长香稻、青珍8号和珍优3号分别持有2个无毒基因;对于品种草笛(Pi-k)、品种20中-1、C104LAC(Pi-1)、C103TTP(Pi-1+1b)、K3(Pi-kh)、梅雨明(Pi-km)和东农363分别持有2个以上无毒基因;亲本菌株对于品种四丰43、珍龙13、C101LAC和20中-2分别持有2个无毒基因,关东51、欧244持有2个以上的无毒基因,但因亲本菌株对品种的致病型一致,难以判断控制菌株对品种非亲和性的基因来源。王艳丽 KAYE Claudia ADREIT Henri MILLAZZO Jo■lle 郑小波 沈瑛 THARREAU Didier 2005中国农业科学2005,38,12:1
4CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae显示文摘Rice genes OsDjA2 and OsERF104,encoding a chaperone protein and an APETELA2/ethylene-responsive factor,respectively,are strongly induced in a compatible interaction with blast fungus,and also have function in plant susceptibility validated through gene silencing.Here,we reported the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance.A total of 15 OsDjA2(62.5%)and 17 OsERF104(70.8%)T_(0)transformed lines were identified from 24 regenerated plants for each target and used in downstream experiments.Phenotyping of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area,compared with the infected control plants.Our results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease.Fabiano T.P.KTÁVORA Anne Cécile MEUNIER Aurore VERNET Murielle PORTEFAIX Joëlle MILAZZO Henri ADREIT Didier THARREAU Octávio LFRANCO Angela MEHTA 2022Rice science2022,29,6:0
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