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| 1 | A model - based method for computer-aided medical decision-making显示文摘 | WEISS SM KULIKOWSKI CA AMAREL S | 1978 | Artificial Intelligence1978，11，： | 1 |
| 2 | Recombinant human leptin treatment in genetic lipodystrophic syndromes: the long (9 term Spanish experience 显示文摘 | ARAUJO-VILAR D SANCHEZ-IGLESIAS S GUILLIN- AMARELLE C | 2015 | Endocrine2015，49，1： | 1 |
| 3 | Identification of Antarctic culturable bacteria able to produce diverse enzymes of potential biotechnological interest显示文摘It is estimated that more than three quarters of the Earth's biosphere is in perennially cold environments. Despite the extreme environmental conditions of desiccation and freezing, microbes can colonize these habitats through the adaptation of metabolic functions and the synthesis of structurally adapted enzymes. Enzymes within psychrophilic microbes exhibit high specific activity at low and moderate temperature, with low thermostability. In this study we used a classic microbiological approach to isolate Antarctic bacteria with cellulolytic, lipolytic, and ligninolytic activities. From 15 different environmental samples, we generated a collection of approximately 800 bacterial isolates that could grow on R2 A or Marine medium at 4℃. This collection was then screened for the presence of the three types of activity at 4℃. We found that 47.7% of the isolates displayed lipolytic activity, 10.2% had cellulase/xylanase activity, and 7.7% showed guaiacol oxidase activity. Of these, 10% displayed two different types of activity, while 0.25% displayed all three types of activity. Our results indicate that cold environments represent outstanding resources for bioprospecting and the study of enzymatic adaptation. | Ignacio Ferrés Vanesa Amarelle Francisco Noya Elena Fabiano | 2015 | Advances in Polar Science2015，26，1： | 0 |
| 4 | Construction and screening of a functional metagenomic library to identify novel enzymes produced by Antarctic bacteria显示文摘A metagenomic fosmid library of approximately 52 000 clones was constructed to identify functional genes encoding cold-adapted enzymes. Metagenomic DNA was extracted from a sample of glacial meltwater, collected on the Antarctic Peninsula during the ANTARKOS XXIX Expedition during the austral summer of 2012–2013. Each clone contained an insert of about 35–40 kb, so the library represented almost 2 Gb of genetic information from metagenomic DNA. Activity-driven screening was used to detect the cold-adapted functions expressed by the library. Fifty lipase/esterase and two cellulase-producing clones were isolated, and two clones able to grow on Avicel as the sole carbon source. Interestingly, three clones formed a brown precipitate in the presence of manganese(II). Accumulation of manganese oxides was determined with a leucoberbelin blue assay, indicating that these three clones had manganese-oxidizing activity. To the best of our knowledge, this is the first report of a manganese oxidase activity detected with a functional metagenomic strategy. | Ignacio Ferrés Vanesa Amarelle Francisco Noya Elena Fabiano | 2015 | Advances in Polar Science2015，26，1： | 0 |
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