|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats’ critical-sized mandibular defects显示文摘BACKGROUND Critically sized bone defects represent a significant challenge to orthopaedic surgeons worldwide.These defects generally result from severe trauma or resection of a whole large tumour.Autologous bone grafts are the current gold standard for the reconstruction of such defects.However,due to increased patient morbidity and the need for a second operative site,other lines of treatment should be introduced.To find alternative unconventional therapies to manage such defects,bone tissue engineering using a combination of suitable bioactive factors,cells,and biocompatible scaffolds offers a promising new approach for bone regeneration.AIM To evaluate the healing capacity of platelet-rich fibrin(PRF)membranes seeded with allogeneic mesenchymal bone marrow-derived stem cells(BMSCs)on critically sized mandibular defects in a rat model.METHODS Sixty-three Sprague Dawley rats were subjected to bilateral bone defects of critical size in the mandibles created by a 5-mm diameter trephine bur.Rats were allocated to three equal groups of 21 rats each.Group I bone defects were irrigated with normal saline and designed as negative controls.Defects of group II were grafted with PRF membranes and served as positive controls,while defects of group III were grafted with PRF membranes seeded with allogeneic BMSCs.Seven rats from each group were killed at 1,2 and 4 wk.The mandibles were dissected and prepared for routine haematoxylin and eosin(HE)staining,Masson's trichrome staining and CD68 immunohistochemical staining.RESULTS Four weeks postoperatively,the percentage area of newly formed bone was significantly higher in group III(0.88±0.02)than in groups I(0.02±0.00)and II(0.60±0.02).The amount of granulation tissue formation was lower in group III(0.12±0.02)than in groups I(0.20±0.02)and II(0.40±0.02).The number of inflammatory cells was lower in group III(0.29±0.03)than in groups I(4.82±0.08)and II(3.09±0.07).CONCLUSION Bone regenerative quality of critically sized mandibular bone defects in rats was better promoted by PRF membranes seeded with BMSCs than with PRF membranes alone. | Muhammad A Awadeen Fouad A Al-Belasy Laila E Ameen Mohamad E Helal Mohammed E Grawish | 2020 | World Journal of Stem Cells2020，12，1： | 4 |
| 2 | Occurrence and distribution of metals and porphyrins in Nigerian coal minerals显示文摘The metal contents of Nigerian coal minerals were analyzed using an atomic absorption spectrophotometer. Calcium, Na, and Fe occurred as the major elements with concentrations ranging from 9 782 μg/g for Ca to 432 μg/g for Na whereas K, Mg, Mn, Ni, Cr, Zn, Pb, and Cu, which occurred at trace levels ranged from 673.73 μg/g for Mg to 2.97 μg/g for Mn. The results of the quantitative analysis of porphyrins extracted from the coal minerals showed that Onyeama coal has the highest amount of porphyrins (ca~0.96 μg/g) while Okpara has the lowest (ca~0.30 μg/g). The porphyrins were qualitatively characterized by a combination of thin layer chromatography (TLC), infrared, and ultraviolet-visible spectrophotometers. The results of the mid infrared analysis (MIR) showed the presence of absorption bands at 3 440 cm~1~3 450 cm-1 and 1 640 cm-1~1 680 cm-1 , which are owing to the stretching vibrations of N ─ H and C─ C of aromatics, with C─ H out of plane (oop) bending vibrations at wavenumbers less than 900 cm-1, all of which are characteristic absorptions of porphyrin free base. The ultraviolet-visible data showed prominent peaks at ~400 nm(Soret band) and at wavelength ranges of 535 nm~550 nm(β-band) and 565 nm~ 600 nm (α-band) for the coal porphyrins analyzed. The geochemical significance of the metals and porphyrins in coal minerals are discussed. | OLAJIRE A A AMEEN A B ABDUL-HAMMED M ADEKOLA F A | 2007 | 燃料化学学报2007，35，6： | 4 |
| 3 | Heterogeneity in mouse spasmolytic polypeptide-expressing metaplasia lineages identifies markers of metaplastic progression显示文摘 | Victoria G Weis Josane F Sousa Bonnie J LaFleur Ki Taek Nam Jared A Weis Paul E Finke Nadia A Ameen James G Fox James R Goldenring | 2013 | Gut2013，，9： | 3 |
| 4 | Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma显示文摘AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,Egypt.Fifteen wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal controls.Laboratory investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and p27.Cyclin D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 immuno-reactivity.The expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied cases.Correlation analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC plays a vital role in the development and differentiation of HCC;while,Cyclin E may be a useful marker for monitoring tumor behavior.p21 and p27 can be used as predictive markers for HCC.Furthermore,higher expression of Rb/p105 as well as inverse relation with p21 and histologic grades suggests its important role in hepatic carcinogenesis. | Azza E El Bassiouny Mona M Nosseir Mona K Zoheiry Noha A Ameen Ahmed M Abdel-Hadi Ibrahim M Ibrahim Suher Zada Abdel-Hakeem Saad El-Deen Nora E El-Bassiouni | 2010 | World Journal of Hepatology2010，2，1： | 3 |
| 5 | 为在尖锐的肝失败病人预言差的结果合并动力学显示文摘 Acute liver failure (ALF), also known as fulminant hepatic failure (FHF), is a devastating clinical syndrome with a high mortality of 60%-90%. An early and exact assessment of the severity of ALF together with prediction of its further development is critical in order to determine the further management of the patient. A number of prognostic models have been used for outcome prediction in ALF patients but they are mostly based on the variables measured at one time point, mostly at admission. ALF patients rarely show a static state: rapid progress to a life threatening situation occurs in many patients. Since ALF is a dynamic process, admission values of prognostic variables change over time during the clinical course of the patient. Kumar et al developed a prognostic model [ALF early dynamic (ALFED)] based on early changes in values of variables which predicted outcome. ALFED is a model which seems to be worthwhile to test in ALF patients in other parts of the world with different aetiologies. Since the exact pathophysiology of ALF is not fully known and is certainly complex, we believe that adding promising variables involved in the pathophysiology of ALF to the dynamic approach might even further improve prognostic performance. We agree with Kumar et al that an improved dynamic prognostic model should be based on simplicity (easily to be performed at the bedside) and accuracy. Our comments presented in this paper may be considered as recommendations for future optimization of ALF prediction models. | Robert AFM Chamuleau Kama A Wlodzimirow Ameen Abu-Hanna | 2012 | World Journal of Gastrointestinal Surgery2012，4，12： | 2 |
| 6 | Importance of PPAR alpha for the effects of growth hormone on hepatic lipid and lipoprotein metabolism显示文摘 | Ljungberg A Linden D Ameen C | 2007 | Growth Horm IGF Res2007，17，2： | 1 |
| 7 | The effect of Cd^2+ on the biosynthesis of chlorophyll in leaves of barley plant 显示文摘 | Stobart A K Griffiths W T Ameen B I | 1985 | Plant Physiology1985，63，： | 1 |
| 8 | The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley显示文摘 | Stobart A K GriffithsW T Ameen B I | 1985 | Plant Physiol11985，63，： | 1 |
| 9 | Ultrasound for accurate measurement of invasive breast canser turmor size 显示文摘 | Shoma A Moutamed A Ameen M | 2006 | Breast J2006，12，： | 1 |
| 10 | Residual Activity and Population Effects of Noviflumuron for German Cockroach (Dictyoptera:Blattellidae) Control显示文摘 | Wang CL Kaakeh W | 2005 | J Econ Entomol2005，98，3： | 1 |
| 11 | Erythema gyratum repens and acquired ichthyos s assoc ated with transitional cell carcinoma of the kidney显示文摘 | Ameen M Chopra S Darvay A | 2001 | Clin Exp Dermatol2001，26，6： | 1 |
| 12 | Ultrasound for accurate measurement of invasive breast cancertumor size 显示文摘 | Shoma A Moutamed A Ameen M | 2006 | Breast J2006，12，3： | 1 |
| 13 | Ultrasound for accurate measurement of invasive breast cancertumor size显示文摘 | Shoma A Moutamed A Ameen M | | 0，，03： | 1 |
| 14 | Thermal comfort in the humid tropics-part1:Climate chamber experiments on temperature preferences in Singapore显示文摘 | DE DEAR R J LEOW K G AMEEN A | 1991 | ASHRAE Transactions1991，97，1： | 1 |
| 15 | Thermal comfort in the humid tropics-part2:Climate chamber experiments on thermal acceptability in Singapore显示文摘 | DE DEAR R J LEOW K G AMEEN A | 1991 | ASHRAE Transactions1991，97，1： | 1 |
| 16 | Ultraslumd for accurate measurement of invasive breast cancer tumor size 显示文摘 | Shoma A Moutamed A Ameen M | 2006 | Breast J2006，12，： | 1 |
| 17 | The effect of Cd on the biosynthesis of hlorophyll in leaves of barley显示文摘 | Stobart A K Griffiths W T Ameen - 2Bukhari I | 1985 | Physiol Plant1985，63，： | 1 |
| 18 | The effect of Cd^2+ on the biosynthesis of chlorophyll in leaves of barley显示文摘 | STOBART A K GRIFFITHS W T AMEEN B I | 1985 | Physiol Plant1985，63，3： | 1 |
| 19 | The effects of Cd^2+ on the biosynthesis of chlorophyll in leaves of barley 显示文摘 | STOBART A K GRIFITHS W T AMEEN BUKHARI | 1985 | Physiologia Plantarum1985，63，： | 1 |
| 20 | Modeling of concrete cracking: A hybrid technique of using displacement discontinuity element method and direct boundary element method显示文摘 | Ameen M Raghu B K Gopalakrishnan A R | 2012 | Engineering Fracture Mechanics2012，35，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员